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Dergiler / Ekin Journal of Crop Breeding and Genetics / 2025 / Volume: 11 Issue: 2

Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures

Selcen Doğan(Hektaş Seed)
Cansu Şimşek(Hektaş Seed)
Murat X Murat Selçuk(Hektaş Seed)
Hasan Pinar(Erciyes University, Faculty of Agriculture, Department of Horticulture)
Nedim Mutlu(Akdeniz University, Faculty of Agriculture, Department of Agricultural Biotechnology)
Ş. Şebnem Ellialtioğlu(Doqutech Academy Llc. Co.)
Sayfa
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Yayın Tarihi
2025-07-31
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Overall when compared to Silor types, Beith Alpha types exhibited a more favorable response in terms of plant regeneration and embryo development, depending on genotype.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Haploids and doubled haploids play a crucial role in the process of plant breeding.
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Temel Bulgular & Veri:The objective of this study was to optimize culture media conditions for gynogenesis induction in vitro to broaden morphogenetic possibilities and accelerate the creation of homozygous lines.
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Sonuç & Katkı:Overall when compared to Silor types, Beith Alpha types exhibited a more favorable response in terms of plant regeneration and embryo development, depending on genotype.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Gynogenesis · medium compositions · haploid

Abstract

Haploids and doubled haploids play a crucial role in the process of plant breeding. Ovary culture has proven to be an effective method for generating double haploid (DH) plants in cucumbers. The objective of this study was to optimize culture media conditions for gynogenesis induction in vitro to broaden morphogenetic possibilities and accelerate the creation of homozygous lines. The effects of seven different induction culture media on embryo and plant development in the ovary culture of Beith Alpha-type and Silor-type cucumber (Cucumis sativus L.) were investigated. Ovary culture experiments were conducted during the fall and spring seasons with 26 Beith Alpha-type and 11 Silor-type cucumber genotypes at the F2 and F3 generations. The results revealed that the most successful outcomes were obtained from genotypes coded HTB20, HTB24 and HTB25 of the Beith Alpha-type and HTS4 and HTS5 of the Silor-type. The findings also demonstrated that explants cultured in the autumn season showed higher regeneration efficiency compared to those cultured in spring. Overall when compared to Silor types, Beith Alpha types exhibited a more favorable response in terms of plant regeneration and embryo development, depending on genotype.

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Bu Makaleye Atıf Yap

Doğan, S., Şimşek, C., Selçuk, M. X. M., Pinar, H., Mutlu, N., Ellialtioğlu, Ş. Ş. (2025). Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures. Ekin Journal of Crop Breeding and Genetics, Volume: 11 Issue: 2. https://ajindex.com/articles/1477728

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