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Dergiler / Ekin Journal of Crop Breeding and Genetics / 2026 / Volume: 12 Issue: 2

Evaluation of Low Temperature Tolerance in Grafted Cucumber Seedlings with Interspecific Hybrid Cucurbita Rootstocks (C. maxima × C. moschata) Using Morphological and Biochemical Indicators

Seda Atasoy
Emre İpek
Sibel Bahadır
Büşra Altuntaş(Petektar Seed R&D Center)
Ahmet Balkaya(ONDOKUZ MAYIS UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE)
Süleyman Kavak(ISPARTA UNIVERSITY OF APPLIED SCIENCES, FACULTY OF AGRONOMICS AND AGRICULTURAL TECHNOLOGIES, DEPARTMENT OF HORTICULTURE, DEPARTMENT OF HORTICULTURE)
Şeküre Şebnem Ellialtioğlu(Doqutech Academy Llc. Co.)
Sayfa
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Yayın Tarihi
2026-08-01
DOI
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Based on these findings, the combinations KB20, KB4, and KB5, identified as tolerant, will be further evaluated for plant growth and yield performance under coldseason greenhouse conditions in future studies.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The use of grafted seedlings enhances water and nutrient uptake under abiotic stress conditions, such as high or low temperatures and drought, by promoting a stronger root system.
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Temel Bulgular & Veri:Additionally, grafting improves tolerance to diseases and pests, thereby positively affecting both yield and quality.
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Sonuç & Katkı:Based on these findings, the combinations KB20, KB4, and KB5, identified as tolerant, will be further evaluated for plant growth and yield performance under coldseason greenhouse conditions in future studies.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Cucurbita rootstock · Grafted seedling · Low-temperature stress

Abstract

The use of grafted seedlings enhances water and nutrient uptake under abiotic stress conditions, such as high or low temperatures and drought, by promoting a stronger root system. Additionally, grafting improves tolerance to diseases and pests, thereby positively affecting both yield and quality. Hybrids of C. moschata × C. moschata and C. maxima × C. moschata are currently the most commonly used rootstocks for cucumber (Cucumis sativus L.) seedling production. In this study, the cucumber cultivar PTK40 F1 was used as the scion, while non-grafted PTK40 F1 seedlings served as the control group. In this study, 11 promising interspecific hybrid Cucurbita rootstocks tolerant of low temperatures were used. The performance of the promising rootstock candidates was compared with that of commercial rootstocks such as Shintoza F 1, Nun 9075 F1, Cobalt F1, Cremna F1, and Max Crom F1. Grafted and non-grafted seedlings were acclimated at 15°C/10°C (day/night) after reaching the 3-4 true-leaf stage. Subsequently, they were subjected to a low-temperature treatment at 5°C (day and night) for two days. Plant damage was evaluated visually using a scale ranging from 0 (no damage) to 5 (dead plant). The results showed that the rootstock/scion combinations KB20/PTK40 F 1, KB17/PTK40 F1, KB19/PTK40 F1, and KB22/PTK40 F1 exhibited high visual tolerance to low temperatures with low damage scores (0-1). However, biochemical analyses revealed significant physiological differences. Under lowtemperature stress, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels generally increased. Contrary to visual observations, KB22 exhibited high lipid peroxidation (MDA: 20.39 nmol g-1 FW). The physiologically most tolerant genotypes were identified as KB5/PTK40 F1 (MDA: 9.99 nmol g-1 FW) and KB4/PTK40 F1 (MDA: 10.73 nmol g-1 FW), which maintained the lowest oxidative damage and did not require excessive proline accumulation. KB20/PTK40 F 1 also demonstrated a balanced tolerance mechanism. Based on these findings, the combinations KB20, KB4, and KB5, identified as tolerant, will be further evaluated for plant growth and yield performance under coldseason greenhouse conditions in future studies.

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Bu Makaleye Atıf Yap

Atasoy, S., İpek, E., Bahadır, S., Altuntaş, B., Balkaya, A., Kavak, S., Ellialtioğlu, Ş. Ş. (2026). Evaluation of Low Temperature Tolerance in Grafted Cucumber Seedlings with Interspecific Hybrid Cucurbita Rootstocks (C. maxima × C. moschata) Using Morphological and Biochemical Indicators. Ekin Journal of Crop Breeding and Genetics, Volume: 12 Issue: 2. https://ajindex.com/articles/1477711

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Kaynakça (38)

  1. Amin, B., Atif, M. J., Meng, H., Ali, M., Li, S., Alharby, H. F., Majrashi, A., Hakeem, K. R., and Cheng, Z. (2022). Melatonin rescues photosynthesis and triggers antioxidant defense response in Cucumis sativus plants challenged by low temperature and high humidity. Frontiers in Plant Science, 13, 855900. https://doi. org/10.3389/fpls.2022.855900
  2. Ashraf, M., Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59(2), 206-216. https:// doi.org/10.1016/j.envexpbot.2005.12.006
  3. Balkaya, A. (2014). Aşılı sebze üretiminde kullanılan anaçlar. TÜRKTOB Türkiye Tohumcular Birliği Dergisi, 3(10), 4-7. (In Turkish)
  4. Blum, A., Jordan, W. R. (1985). Breeding crop varieties for stress environments. Critical Reviews in Plant Sciences, 2(3), 199-238. https://doi. org/10.1080/07352688509382196
  5. Bulder, H. A. M., den Nijs, A. P. M., Speek, E. J., Hasselt, P. R., and Kuiper, P. J. C. (1991). The effect of low root temperature on growth and lipid composition of low temperature tolerant rootstock genotypes for cucumber. Journal of Plant Physiology, 38(6), 661-666. https://doi. org/10.1016/S0176-1617(11)81312-X
  6. Campos, P. S., Quartin, V., Ramalho, J. C., and Nunes, M. A. (2003). Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp. plants. Journal of Plant Physiology, 160(3), 283-292. https://doi.org/10.1078/0176- 1617-00833
  7. den Nijs, A.P. M. (1981). Adaptation of the glasshouse cucumber to lower temperatures in winter by breeding. Acta Horticulturae, 118, 65-72. https:// doi.org/10.17660/ActaHortic.1981.118.8
  8. Food and Agriculture Organization of the United Nations. (2025). FAOSTAT [Data set]. Retrieved January 15, 2025, from https://www.fao.org/ faostat
  9. Fu, X., Feng, Y., Zhang, Y., Bi, H., and Ai, X. (2024). Salicylic acid improves chilling tolerance via CsNPR1–CsICE1 interaction in grafted cucumbers. Horticulture research, 11(10), uhae231. https://doi.org/10.1093/hr/uhae231
  10. Ghosh, U. K., Islam, M. N., Siddiqui, M. N., Cao, X., and Khan, M. A. R. (2022). Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biology, 24(2), 227-239. https://doi.org/10.1111/plb.13363
  11. Gill, S. S., Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48(12), 909-930. https://doi. org/10.1016/j.plaphy.2010.08.016
  12. Gomez, K. A., Gomez, A. A. (1984). Statistical procedures for agricultural research. John Wiley and Sons.
  13. Güneri Bağcı, E. (2010). Determination of oxidative stress induced by drought in chickpea (Cicer arietinum L.) cultivars by physiological and biochemical parameters [Doctoral dissertation, Ankara University]. (In Turkish)
  14. Hasanuzzaman, M., Bhuyan, M. H. M. B., Zulfiqar, F., Raza, A., Mohsin, S. M., Mahmud, J. A., Fujita, M., and Fotopoulos, V. (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants, 9(8), 681. https://doi.org/10.3390/antiox9080681
  15. Heath, R. L., Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125(1), 189-198. https://doi.org/10.1016/0003- 9861(68)90654-1
  16. Hodges, D. M., DeLong, J. M., Forney, C. F., and Prange, R. K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207(4), 604-611. https://doi.org/10.1007/ s004250050524
  17. Huang, Y., Li, J., Hua, B., Liu, Z., Fan, M., and Bie, Z. (2013). Grafting onto different rootstocks as a means to improve watermelon tolerance to low potassium stress. Scientia Horticulturae, 149, 80- 85. https://doi.org/10.1016/j.scienta.2012.02.009
  18. İpek, E., Bahadır, S., Yapici, B., Atasoy, S., and Balkaya, A. (2025). Düşük sıcaklığa toleranslı türler arası hibrit kabak anaçlarının aşılı hıyar fide üretimindeki kullanılabilirliğinin değerlendirilmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 30(3), 692-702. https:// doi.org/10.37908/mkutbd.1695923
  19. Karaağaç, O., Kar, H., Murat Doğru, Ş., Özbakır Özer, M., and Demir, E. (2017). Örtüaltı hıyar (Cucumis sativus L.) yetiştiriciliğine uygun düşük sıcaklığa toleranslı yerli hibrit kabak (Cucurbita spp.) anaçlarının geliştirilmesi ve düşük sıcaklığa dayanıklılığın fizyolojik ve biyokimyasal düzeyde incelenmesi ( Project No. 114O843). TÜBİTAK.
  20. Kaur, G., Asthir, B. (2015). Proline: a key player in plant abiotic stress tolerance. Biologia Plantarum, 59(4), 609-619. https://doi.org/10.1007/s10535- 015-0549-3
  21. Kozak, M., Piepho, H. P. (2018). What’s normal anyway? Residual plots are more telling than significance tests when checking ANOVA assumptions. Journal of Agronomy and Crop Science, 204(1), 86-98.
  22. Lee, J. M., Oda, M. (2003). Grafting of herbaceous vegetable and ornamental crops. Horticultural Reviews, 28, 61-124.
  23. Lee, J. M., Kubota, C., Tsao, S. J., Bie, Z., Echevarria, P. H., Morra, L., and Oda, M. (2010). Current status of vegetable grafting: Diffusion, grafting techniques, automation. Scientia Horticulturae, 127(2), 93-105. https://doi.org/10.1016/j. scienta.2010.08.003
  24. Li, Y., Tian, X., Wei, M., Shi, Q., Yang, F., and Wang, X. (2015). Mechanisms of tolerance differences in cucumber seedlings grafted on rootstocks with different tolerance to low temperature and weak light stresses. Turkish Journal of Botany, 39(4), 606-614. https://doi.org/10.3906/bot-1404-115
  25. Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7(9),405-410.
  26. Ohkawa, H., Ohishi, N., and Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351-358. https://doi.org/10.1016/0003- 2697(79)90738-3
  27. Schwarz, D., Rouphael, Y., Colla, G., and Venema, J. H. (2010). Grafting as a tool to improve tolerance of vegetables to abiotic stresses: Thermal stress, water stress and organic pollutants. Scientia Horticulturae, 127(2), 162-171. https://doi. org/10.1016/j.scienta.2010.09.016
  28. Slesak, I., Libik, M., Karpinska, B., Karpinski, S., and Miszalski, Z. (2007). The role of hydrogen peroxide in regulation of plant metabolism and cellular signalling in response to environmental stresses. Acta Biochimica Polonica, 54(1), 39-50.
  29. Smeets, L., Wehner, T. C. (1997). Environmental effects on genetic variation of chilling resistance in cucumber. Euphytica, 97(2), 217-225. https:// doi.org/10.1023/A:1003084821178
  30. Sun, S., Yang, Y., Hao, S., Liu, Y., Zhang, X., Zhang, X., and Luo, Y. (2024). Comparison of transcriptome and metabolome analysis revealed cold-resistant metabolic pathways in cucumber roots under lowtemperature stress in root zone. Frontiers in Plant Science, 15, 1413716. https://doi.org/10.3389/ fpls.2024.1413716
  31. Szabados, L., Savouré, A. (2010). Proline: a multifunctional amino acid. Trends in Plant Science, 15(2), 89-97.
  32. Tachibana, S. (1982). Comparison of effects of root temperature on growth and mineral nutrition of cucumber cultivars and figleaf gourd. Journal of the Japanese Society for Horticultural Science, 51(3), 299-308. https://doi.org/10.2503/ jjshs.51.299
  33. Tachibana, S. (1988). Cytokinin concentrations in roots and root xylem exudate of cucumber and figleaf gourd as affected by root temperature. Journal of the Japanese Society for Horticultural Science, 56(4), 417-425. https://doi.org/10.2503/ jjshs.56.417
  34. Turkish Statistical Institute. (2024). Crop production statistics [Data set]. Retrieved January 15, 2025, from https://data.tuik.gov.tr/
  35. Xiao, H., Deng, W., Ahmad, B., Guo, C., Shi, S., He, S., and Yang, Z. A. (2025). Cucurbita ficifolia rootstock enhances resistance to low-temperature stress in cucumber. Horticulturae, 11(3), 242. https://doi.org/10.3390/horticulturae11030242
  36. Yücel, Ş., Karaağaç, O., Balkaya, A., and Kandemir, D. (2022). Aşılı fide üretiminde kullanılan anaçlar. In H. Yetişir and Ş. Ş. Ellialtıoğlu (Eds.), Sebzelerde Fide Yetiştiriciliği-2 (pp. 399-490). Gece Kitaplığı.
  37. Zhou, Y. H., Huang, L. F., Zhang, Y. L., Shi, K., Yu, J. Q., and Nogués, S. (2007). Chill-induced decrease in capacity of RuBP carboxylation and associated H₂O₂ accumulation in cucumber leaves are alleviated by grafting onto figleaf gourd. Annals of Botany, 100(4), 839-848. https://doi. org/10.1093/aob/mcm181
  38. Zulfiqar, F., Akram, N. A., and Ashraf, M. (2020). Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon. Planta, 251(1), 3. https://doi.org/10.1007/s00425-019- 03293-1