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Dergiler / Anatomy / 2016 / Cilt: 10 - Sayı: 3

Reduction in Wnt9b and associated gene expression in the embryonic midface of CL/Fr mice with heritable cleft lip and palate

Brennan TAKAGİ
Trudy HONG
Thomas E HYND
Keith S K FONG
Ben FOGELGREN
Kazuaki NONAKA
Scott LOZANOFF
Dergi
Anatomy
Sayfa
182–192
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Misexpression of Sst suggests that it may be a downstream target of Wnt9b causing reduced overall growth possibly hindering fusion of facial prominences and contributing to the development of CLP.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objectives: The CL/Fr mouse displays cleft lip and palate (CLP) at a rate of 35%.
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Temel Bulgular & Veri:The clf1 mutation is associated with CLP in related“A” strain mice and affects the gene Wnt9b.
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Sonuç & Katkı:Misexpression of Sst suggests that it may be a downstream target of Wnt9b causing reduced overall growth possibly hindering fusion of facial prominences and contributing to the development of CLP.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:cleft lip and palate · CL/Fr mice · craniofacial

Abstract

Objectives: The CL/Fr mouse displays cleft lip and palate (CLP) at a rate of 35%. The clf1 mutation is associated with CLP in related“A” strain mice and affects the gene Wnt9b. The purpose of this study was to determine tissue specific expression of Wnt9bduring facial prominence morphogenesis in CL/Fr mice and provide new details concerning gene variants associated with CLP.Methods: Facial prominences from CLP(-) and CLP(+) CL/Fr and 3H1 wild-type (WT) mice at embryonic day 11.5 (E11.5)were collected for expression assays (DNA microarray analysis, qRT-PCR, immunostaining, and in situ hybridization). A modified Chi square test was used to analyze microarray data while a student t-test was used to statistically compare qRT-PCR values (p<0.05).Results: There was a partial and variable loss of Wnt9b in facial prominences of E11.5 CLP susceptible CL/Fr mice, with a greater loss associated with CLP(+). Two genes in the clf2 locus, Adcy2 and Ube2q11 also showed decreased expression. Two regulators of palatogenesis, Runx2 and Osr2 were significantly downregulated, while an inhibitor of cell proliferation, somatostatin (Sst), was elevated in CLP(+) relative to CLP(-) mice.Conclusion: Results indicate a role for Wnt9b in the pathogenesis of CLP and supports previous reports concerning its involvement with CLP in “A” strain mice. Misexpression of Sst suggests that it may be a downstream target of Wnt9b causing reduced overall growth possibly hindering fusion of facial prominences and contributing to the development of CLP.

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Bu Makaleye Atıf Yap

TAKAGİ, B., HONG, T., HYND, T. E., FONG, K. S. K., FOGELGREN, B., NONAKA, K., LOZANOFF, S. (2016). Reduction in Wnt9b and associated gene expression in the embryonic midface of CL/Fr mice with heritable cleft lip and palate. Anatomy, Cilt: 10 - Sayı: 3, 182–192. https://ajindex.com/articles/147077

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