Yapay Zeka Hızlı Bakış & Çıkarımlar
AI InsightWhile no significant correlation with prognostic parameters was observed in our limited cohort, further multicenter studies with larger, heterogeneous patient groups are warranted.
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Özet
Hepatocellular carcinoma (HCC) represents one of the most prevalent primary liver malignancies. The activation of the Wnt/β-catenin signaling pathway is frequently observed in HCC, contributing to tumor progression. Dynamin-related protein-1 (DRP-1) is involved in apoptosis, tumor metastasis, and suppression of cell adhesion and migration. While DRP-1 and β-catenin have been reported in various tumors, their significance in HCC remains controversial. This study investigates the immunohistochemical (IHC) expression of β-catenin and DRP-1 in HCC. Thirty HCC cases diagnosed within the last 15 years at our institution were included. IHC staining with β-catenin and DRP-1 antibodies was performed on paraffin-embedded tumor and non-tumor tissue samples. Thus, the expressions of these proteins were analyzed. Among 30 cases, 25 had a cirrhotic background, while 5 were non-cirrhotic. IHC analysis revealed membranous β-catenin reactivity in tumor areas in 29 cases, with one case exhibiting both membranous and nuclear reactivity. Weaker immunoreactivity was observed in non-tumoral hepatocytes and biliary epithelium compared to tumor areas. DRP-1 expression was absent in non-tumoral hepatocytes, while cytoplasmic immunoreactivity was statistically strong in 16 cases (53%) (P=0.0008). No correlation was found between nuclear grade, lymphovascular invasion, serum AFP levels, necrosis, survival, and β-catenin or DRP-1 expression (P>0.05). Our findings suggest a potential role for DRP-1 in HCC pathogenesis. While no significant correlation with prognostic parameters was observed in our limited cohort, further multicenter studies with larger, heterogeneous patient groups are warranted.
Abstract
Hepatocellular carcinoma (HCC) represents one of the most prevalent primary liver malignancies. The activation of the Wnt/β-catenin signaling pathway is frequently observed in HCC, contributing to tumor progression. Dynamin-related protein-1 (DRP-1) is involved in apoptosis, tumor metastasis, and suppression of cell adhesion and migration. While DRP-1 and β-catenin have been reported in various tumors, their significance in HCC remains controversial. This study investigates the immunohistochemical (IHC) expression of β-catenin and DRP-1 in HCC. Thirty HCC cases diagnosed within the last 15 years at our institution were included. IHC staining with β-catenin and DRP-1 antibodies was performed on paraffin-embedded tumor and non-tumor tissue samples. Thus, the expressions of these proteins were analyzed. Among 30 cases, 25 had a cirrhotic background, while 5 were non-cirrhotic. IHC analysis revealed membranous β-catenin reactivity in tumor areas in 29 cases, with one case exhibiting both membranous and nuclear reactivity. Weaker immunoreactivity was observed in non-tumoral hepatocytes and biliary epithelium compared to tumor areas. DRP-1 expression was absent in non-tumoral hepatocytes, while cytoplasmic immunoreactivity was statistically strong in 16 cases (53%) (P=0.0008). No correlation was found between nuclear grade, lymphovascular invasion, serum AFP levels, necrosis, survival, and β-catenin or DRP-1 expression (P>0.05). Our findings suggest a potential role for DRP-1 in HCC pathogenesis. While no significant correlation with prognostic parameters was observed in our limited cohort, further multicenter studies with larger, heterogeneous patient groups are warranted.