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Dergiler / Black Sea Journal of Engineering and Science / 2026 / Cilt: 8 Sayı: 4

Development and Validation of a Simple HPLC-UV Method for Analysis of Harmine

Galip Mesut Demir(ATATÜRK ÜNİVERSİTESİ)0000-0001-6107-2604
Afife Büşra Uğur Kaplan(ATATÜRK ÜNİVERSİTESİ)0000-0003-2222-8789
Mine Gülaboğlu(ATATÜRK ÜNİVERSİTESİ)0000-0002-3248-1502
Meltem Çetin(ATATÜRK ÜNİVERSİTESİ)0000-0003-4009-2432
Sayfa
1096–1102
Yayın Tarihi
2025-07-15
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It can be widely used in routine laboratory practices for the monitoring of HRM in pure form and in various pharmaceutical formulations.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In this study, a rapid, simple, and sensitive HPLC-UV method was developed and validated in accordance with ICH Q2 (R1) guideline for the quantitative analysis of harmine (HRM), a main β-carboline alkaloid found in the Peganum harmala (Uzerlik) plant.
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Temel Bulgular & Veri:The method was established based on the UV spectral analysis, which indicated maximum absorbance for HRM at 243 nm.
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Sonuç & Katkı:It can be widely used in routine laboratory practices for the monitoring of HRM in pure form and in various pharmaceutical formulations.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Harmine · HPLC-UV method · Peganum harmala

Özet

In this study, a rapid, simple, and sensitive HPLC-UV method was developed and validated in accordance with ICH Q2 (R1) guideline for the quantitative analysis of harmine (HRM), a main β-carboline alkaloid found in the Peganum harmala (Uzerlik) plant. The method was established based on the UV spectral analysis, which indicated maximum absorbance for HRM at 243 nm. A mixture of methanol and ultrapure water containing 1% formic acid (50:50, v/v) was selected as the mobile phase. In the separation carried out under isocratic conditions, the retention time of HRM was determined as 5.5 min, and the entire analysis was completed in under 8 min. The method demonstrated excellent linearity within the concentration range of 1–36 µg/mL, with a correlation coefficient (R²) of 0.99998. The accuracy and precision parameters of the method were found to be within acceptable limits, with relative error (RE) and relative standard deviation (RSD%) both below 2. The limits of detection and quantification (LOD and LOQ) were 0.269 µg/mL and 0.816 µg/mL, respectively. Also, it was determined that HRM standard solutions were stable for up to 7 days when stored at different temperatures (−20°C, 2–8°C, 25°C). In conclusion, this method offers a reliable alternative for the quantitative determination of HRM with high analytical quality, reproducibility, and short time. It can be widely used in routine laboratory practices for the monitoring of HRM in pure form and in various pharmaceutical formulations.

Abstract

In this study, a rapid, simple, and sensitive HPLC-UV method was developed and validated in accordance with ICH Q2 (R1) guideline for the quantitative analysis of harmine (HRM), a main β-carboline alkaloid found in the Peganum harmala (Uzerlik) plant. The method was established based on the UV spectral analysis, which indicated maximum absorbance for HRM at 243 nm. A mixture of methanol and ultrapure water containing 1% formic acid (50:50, v/v) was selected as the mobile phase. In the separation carried out under isocratic conditions, the retention time of HRM was determined as 5.5 min, and the entire analysis was completed in under 8 min. The method demonstrated excellent linearity within the concentration range of 1–36 µg/mL, with a correlation coefficient (R²) of 0.99998. The accuracy and precision parameters of the method were found to be within acceptable limits, with relative error (RE) and relative standard deviation (RSD%) both below 2. The limits of detection and quantification (LOD and LOQ) were 0.269 µg/mL and 0.816 µg/mL, respectively. Also, it was determined that HRM standard solutions were stable for up to 7 days when stored at different temperatures (−20°C, 2–8°C, 25°C). In conclusion, this method offers a reliable alternative for the quantitative determination of HRM with high analytical quality, reproducibility, and short time. It can be widely used in routine laboratory practices for the monitoring of HRM in pure form and in various pharmaceutical formulations.

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Bu Makaleye Atıf Yap

Demir, G. M., Kaplan, A. B. U., Gülaboğlu, M., Çetin, M. (2025). Development and Validation of a Simple HPLC-UV Method for Analysis of Harmine. Black Sea Journal of Engineering and Science, Cilt: 8 Sayı: 4, 1096–1102. https://ajindex.com/articles/1467124

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