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Dergiler / Biyolojik Çeşitlilik ve Koruma / 2025 / Volume: 18 Issue: 3

Bazı Antrakinon Türevleri ile HeLa Hücrelerinde Mitokondriyal (İntrinsik) Yolak Üzerinden Apoptozun Uyarılması

The stimulation of apoptosis via mitochondrial (intrinsic) pathway in HeLa cells by some anthraquinone derivates

Elif Apaydın(ANADOLU UNIVERSITY)0000-0002-2544-4068
İpek Nur Önder(ANADOLU ÜNİVERSİTESİ)0009-0002-0954-5501
Zerrin İncesu(ANADOLU ÜNİVERSİTESİ)0000-0001-6042-3812
Filiz Özdemir(ANADOLU ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, BİYOKİMYA ANABİLİM DALI)0000-0002-3359-4496
Sayfa
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Yayın Tarihi
2025-12-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: Ant-6 (3 μM) ve Ant-4 (5 μM) ile 6 saatlik inkübasyon sonrası maksimum Kaspaz-9 aktivasyonu gözlenmiştir.

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Amaç & Hipotez:Amaç: Bu çalışmanın amacı, Ant-3, -4 ve -6 antrakinon türevlerinin HeLa hücrelerinde mitokondriyal (intrinsik) apoptotik yolak üzerindeki etkilerini araştırmaktır.
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Temel Bulgular & Veri:Yöntemler: Mitokondriyal membran potansiyeli değişiklikleri, Kaspaz-9 aktivitesi, apoptotik aktivite ve intrinsik yolağa dahil olan proteinlerin ekspresyon seviyeleri ölçülmüştür.
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Sonuç & Katkı:Bulgular: Ant-6 (3 μM) ve Ant-4 (5 μM) ile 6 saatlik inkübasyon sonrası maksimum Kaspaz-9 aktivasyonu gözlenmiştir.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Anthraquinone · apoptosis · caspase-9

Özet

Amaç: Bu çalışmanın amacı, Ant-3, -4 ve -6 antrakinon türevlerinin HeLa hücrelerinde mitokondriyal (intrinsik) apoptotik yolak üzerindeki etkilerini araştırmaktır. Yöntemler: Mitokondriyal membran potansiyeli değişiklikleri, Kaspaz-9 aktivitesi, apoptotik aktivite ve intrinsik yolağa dahil olan proteinlerin ekspresyon seviyeleri ölçülmüştür. Apoptotik indeksin belirlenmesinde TUNEL yöntemi kullanılmıştır. Bulgular: Ant-6 (3 μM) ve Ant-4 (5 μM) ile 6 saatlik inkübasyon sonrası maksimum Kaspaz-9 aktivasyonu gözlenmiştir. Mitokondriyal membran potansiyelindeki en yüksek değişiklik, Ant-4 (5 μM) ile 36 saatlik uygulama sonrası meydana gelmiştir. TUNEL yöntemiyle belirlenen apoptotik indeks, Ant-4 (5 μM) grubunda 48 saat sonunda en yüksek seviyeye ulaşmıştır. Ant-4 ve mitoksantron ile tedavi edilen HeLa hücrelerinde 48 saat sonunda AIF ve EndoG proteinlerinin ekspresyonunda artış gözlenmiştir. Sonuç: Bu bulgular, in vitro deneylerde umut verici sonuçlar ortaya koymaktadır. Bununla birlikte, bu yeni sentezlenen antrakinon türevlerinin rahim ağzı kanseri tedavisindeki terapötik potansiyelini doğrulamak için ileri klinik çalışmalara ihtiyaç vardır.

Abstract

Objective: The aim of this study was to investigate the effects of Ant-3, -4, and -6 anthraquinone derivatives on the mitochondrial (intrinsic) apoptotic pathway in HeLa cells. Methods: Mitochondrial membrane potential changes, Caspase-9 activity, apoptotic activity, and protein expression levels involved in the intrinsic pathway were measured. The TUNEL method was used to determine the apoptotic index. Results: Maximum Caspase-9 activation was observed after 6 hours of incubation with Ant-6 (3 μM) and Ant-4 (5 μM).The highest mitochondrial membrane potential change occurred after 36 hours of treatment with Ant-4 (5 μM).The apoptotic index, determined by the TUNEL method, peaked in the Ant-4 (5 μM) group after 48 hours. Increased expression of AIF and EndoG proteins was observed after 48 hours in HeLa cells treated with Ant-4 and mitoxantrone. Conclusion: These findings show promising results in in vitro experiments. However, further clinical studies are needed to validate the therapeutic potential of these newly synthesized anthraquinone derivatives in the treatment of cervical cancer.

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Bu Makaleye Atıf Yap

Apaydın, E., Önder, İ. N., İncesu, Z., Özdemir, F. (2025). Bazı Antrakinon Türevleri ile HeLa Hücrelerinde Mitokondriyal (İntrinsik) Yolak Üzerinden Apoptozun Uyarılması. Biyolojik Çeşitlilik ve Koruma, Volume: 18 Issue: 3. https://doi.org/10.46309/biodicon.2025.1652554

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