Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 3

Investigation of Antigenic Specificity against Cysticercus tenuicollis Cyst Fluid Antigen in Dogs Experimentally Infected with Taenia hydatigena

Sayfa
835–840
Yayın Tarihi
2005-03-01
DOI
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According to the results of this study performed with fluid antigen from C. tenuicollis, the bands of 38 and 42.5 kDa are concluded to be the specific proteins for diagnosis of T. hydatigena in dogs.

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Amaç & Hipotez:Antigenic specificity was investigated in dogs experimentally infected with Taenia hydatigena using SDS-PAGE and Western blotting.
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Temel Bulgular & Veri:Experimental infection was achieved in the animals of the experimental group at 11-13 weeks, and infected animals were necropsied and mature Taenia hydatigena worms were collected.
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Sonuç & Katkı:According to the results of this study performed with fluid antigen from C. tenuicollis, the bands of 38 and 42.5 kDa are concluded to be the specific proteins for diagnosis of T. hydatigena in dogs.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Taenia hydatigena · dog · Western blotting

Özet

Antigenic specificity was investigated in dogs experimentally infected with Taenia hydatigena using SDS-PAGE and Western blotting. Cysticercus tenuicollis cysts from sheep were given to the puppies in the experimental group. Experimental infection was achieved in the animals of the experimental group at 11-13 weeks, and infected animals were necropsied and mature Taenia hydatigena worms were collected. Antigen was prepared from the fluids of C. tenuicollis. Sera from both experimental and control groups were tested by Western blotting. According to the results of this study performed with fluid antigen from C. tenuicollis, the bands of 38 and 42.5 kDa are concluded to be the specific proteins for diagnosis of T. hydatigena in dogs.

Abstract

Antigenic specificity was investigated in dogs experimentally infected with Taenia hydatigena using SDS-PAGE and Western blotting. Cysticercus tenuicollis cysts from sheep were given to the puppies in the experimental group. Experimental infection was achieved in the animals of the experimental group at 11-13 weeks, and infected animals were necropsied and mature Taenia hydatigena worms were collected. Antigen was prepared from the fluids of C. tenuicollis. Sera from both experimental and control groups were tested by Western blotting. According to the results of this study performed with fluid antigen from C. tenuicollis, the bands of 38 and 42.5 kDa are concluded to be the specific proteins for diagnosis of T. hydatigena in dogs.

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Bu Makaleye Atıf Yap

KARA, M. (2005). Investigation of Antigenic Specificity against Cysticercus tenuicollis Cyst Fluid Antigen in Dogs Experimentally Infected with Taenia hydatigena. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 3, 835–840. https://ajindex.com/articles/146353

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Kaynakça (11)

  1. Pamukçu, A.M., Ertürk E.: 1933 ve 1960 yılları arasında Ankara ve yöresinde köpeklerde görülen hastalıklara toplu bir bakıfl. Ankara Univ. Vet. Fak. Derg., 1961; 8: 323-346.
  2. Doğanay, A.: Ankara sokak köpeklerinde görülen helmint türleri, bunların yayılıflı ve halk sağlığı yönünden önemi. Ankara Üniv. Vet. Fak. Derg., 1983; 30: 550-561.
  3. Heath, D.D., Parmeter, S.N., Osborne P.J: An attempt to immunize dogs against 29: 388-389.
  4. Soulsby, E.J.L: Helminths, Arthropods and Protozoa of Domesticated Animals. Bailliere Tindall, London, UK. 1982; 113- 114. Gasser, R.B., Jenkins, D.J., Heath, D.D., Lawrence, S.B.: Use of Echinococcus granulosus worm antigens for immunodiagnosis of E. granulosus infection in dogs. Vet. Parasitol., 1992; 45: 89- 100. 6. Deplazes, P., Eckert, J.: Untersuchungen zur Infektion des hundes mit Taenia hydatigena. Schweiz. Arch. Tierheilk., 1988; 128: 289-306.
  5. Rickard, M.D., Williams, J.F.: Hydatidosis-cyticercosis immune mechanisms and immunization against infection. Adv. Parasitol., 1982; 21: 229-296. 8. Heath, D.D., Lawrence, S.B., Glennie, A., Twaalhoven, H.: The use of excretory and secretory antigens of the scolex of Taenia ovis for the serodiagnosis of infections in dogs. J. Parasitol., 1985; 71: 192-199.
  6. Jenkins, D.J., Rickard, M.D.: Specific antibody responses to Taenia hydatigena, T. pisiformis, and Echinococcus granulosus infection in dogs. Aust. Vet. J., 1985; 62: 72-78.
  7. Jenkins, D.J., Rickard, M.D.: Specificity of scolex and oncosphere antigens for the serological diagnosis of taeniid cestode infections in dogs. Aust. Vet. J., 1986; 63: 40-42.
  8. Jenkins, D.J., Rickard, M.D.: Specific antibody responses in dogs experimentally infected with Echinococcus granulosus. Am. J. Trop. Med. Hyg., 1986; 35: 345-349.
  9. Sambrook, J., Fritsch, E.F., Manniatis, T.: Molecular Cloning: A Laboratory Manual, 15. section, Cold Spring Harbor, New York, USA. 1989; 18.47-18.76. Ito, A., Smyth, J.D.: Adult cestodes-immunology of the lumen- dwelling cestode infections. Ed: E.J.L., Soulsby, Immune Responses in Parasitic Infections: Immune responses in parasitic infections: Immunology, immunopathology and Immunoprophylaxis: Vol: 2, Trematodes and Cestodes. Boca Raton: CRC Press, USA. 1987; 115-163.
  10. Heath, D.D., Parmeter, S.N., Osborne, P.J.: An attempt to immunize dogs against Taenia hydatigena. Res. Vet. Sci., 1980; 29: 388-389.
  11. Neveu-Lemarie, M.: Traite D'Helminthologie Medicale et Veterinaire. Vigot Freres, Editeurs, Paris, France. 1936; 335- 343. Güralp, N.