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Dergiler / Balıkesir Sağlık Bilimleri Dergisi / 2026 / Volume: 14 Issue: 1

Sisplatinin Neden Olduğu Testikular Oksidatif Stres Üzerine Kı̇sspeptı̇n-10'un Etkisi

The Effect of Kisspeptin-10 on Cisplatin-Induced Testicular Oxidative Stress

Gözde Arkalı(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0002-0850-7557
Tutku Can Acısu(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0002-0882-937X
Edanur Güler Ekmen(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0001-8473-7592
Meltem Sağıroğlu(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0001-6547-6809
Fatma Beril Koçyiğit(HARRAN ÜNİVERSİTESİ, BİRECİK MESLEK YÜKSEKOKULU)0000-0001-5659-394X
Mehmet Çay(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0003-3896-0042
Abdurrauf Yüce(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0003-2928-5970
Mesut Aksakal(FIRAT ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER HEKİMLİĞİ TEMEL BİLİMLERİ BÖLÜMÜ, VETERİNERLİK FİZYOLOJİSİ ANABİLİM DALI)0000-0002-6230-2413
Sayfa
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Yayın Tarihi
2025-03-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: Sisplatin grubunda testikular malondialdehit düzeyinin arttığı (p<0.001), katalaz enzim aktivitesinin (p<0.001) ve glutatyon düzeyinin (p<0.01) azaldığı, sperm motilitesinin (p<0.05) ve testis (p<0.01) ile vezikula seminalis (p<0.001) üreme organ ağırlığının azaldığı belirlendi.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Antikanserojen ilaçlardan biri olan sisplatin, spermatogenik hücrelerde, Sertoli hücrelerinde, Leydig hücrelerinde hasara yol açmaktadır.
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Temel Bulgular & Veri:Bu çalışmada, sisplatinin erkek üreme organlarında neden olduğu oksidatif stres ve akabinde gelişen spermatolojik parametreler üzerindeki olumsuz etkilere karşı kisspeptin-10’un etkisinin araştırılması amaçlandı.
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Sonuç & Katkı:Bulgular: Sisplatin grubunda testikular malondialdehit düzeyinin arttığı (p<0.001), katalaz enzim aktivitesinin (p<0.001) ve glutatyon düzeyinin (p<0.01) azaldığı, sperm motilitesinin (p<0.05) ve testis (p<0.01) ile vezikula seminalis (p<0.001) üreme organ ağırlığının azaldığı belirlendi.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:34 adet
⚙️Değişkenler:Cisplatin · Testes · Kisspeptin-10

Özet

Amaç: Antikanserojen ilaçlardan biri olan sisplatin, spermatogenik hücrelerde, Sertoli hücrelerinde, Leydig hücrelerinde hasara yol açmaktadır. Bu çalışmada, sisplatinin erkek üreme organlarında neden olduğu oksidatif stres ve akabinde gelişen spermatolojik parametreler üzerindeki olumsuz etkilere karşı kisspeptin-10’un etkisinin araştırılması amaçlandı. Gereç ve Yöntem: Deneyde 34 adet erkek Sprague Dawley sıçanı kullanıldı. Ratlar kontrol, sisplatin, Kisspeptin-10 ve sisplatin+kisspeptin-10 olarak 4 gruba ayrıldı. Sisplatin 5 mg/kg/tek doz, Kisspeptin-10 ise 50 nmol/kg dozda 7 gün boyunca intraperitoneal uygulandı. Bulgular: Sisplatin grubunda testikular malondialdehit düzeyinin arttığı (p<0.001), katalaz enzim aktivitesinin (p<0.001) ve glutatyon düzeyinin (p<0.01) azaldığı, sperm motilitesinin (p<0.05) ve testis (p<0.01) ile vezikula seminalis (p<0.001) üreme organ ağırlığının azaldığı belirlendi. CP grubu ile kıyaslandığında CP+Kiss-10 grubunda testikular malondialdehit düzeyinin azaldığı (p<0.001), glutatyon düzeyinin arttığı (p<0.01), sperm motilitesinin (p<0.05) ve testis (p<0.01) ile vezikula seminalis (p<0.001) üreme organ ağırlığının arttığı belirlendi. Sonuç: Kisspeptin-10’un sisplatin kaynaklı testikular hasarda etkili olan oksidatif stresi azaltarak, spermatolojik parametreler üzerinde iyileştirici etkiler gösterdiği kanısına varılmıştır.

Abstract

Objective: Cisplatin, one of the anticarcinogenic drugs, causes damage to spermatogenic cells, sertoli cells and leydig cells. This study aimed to investigate the kisspeptin-10 effect against oxidative stress caused by cisplatin in male reproductive organs and its negative effects on spermatological parameters. Materials and Methods: In the experiment, 34 male Sprague Dawley rats were used. Rats were divided into 4 groups as control, cisplatin, Kisspeptin-10 and cisplatin+kisspeptin-10. Cisplatin 5 mg/kg/single dose and Kisspeptin-10 50 nmol/kg dose were administered intraperitoneally for 7 days. Results: In the cisplatin group, testicular malondialdehyde level increased (p<0.001), catalase enzyme activity (p<0.001) and glutathione level (p<0.01) decreased, sperm motility (p<0.05) and testicular (p<0.01) and seminal vesicle (p<0.001) weights decreased. In the cisplatin group administered kisspeptin-10, it was determined that testicular malondialdehyde level decreased (p<0.001), glutathione level increased (p<0.01), sperm motility increased (p<0.05), and testicular (p<0.01) and seminal vesicle (p<0.001) weights increased compared to the cisplatin group. Conclusion: As a result, it was revealed that Kisspeptin-10 showed improving effects on spermatological parameters by reducing oxidative stress that is effective in cisplatin-induced testicular damage.

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Bu Makaleye Atıf Yap

Arkalı, G., Acısu, T. C., Ekmen, E. G., Sağıroğlu, M., Koçyiğit, F. B., Çay, M., Yüce, A., Aksakal, M. (2025). Sisplatinin Neden Olduğu Testikular Oksidatif Stres Üzerine Kı̇sspeptı̇n-10'un Etkisi. Balıkesir Sağlık Bilimleri Dergisi, Volume: 14 Issue: 1. https://doi.org/10.53424/balikesirsbd.1609836

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