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Dergiler / Balıkesir Sağlık Bilimleri Dergisi / 2026 / Volume: 15 Issue: 3

Effects of Permethrin on Oxidative Stress, Apoptosis, Mitochondrial Ultrastructure in Rat Testes

Tuğba Ekiz Yılmaz(İSTANBUL ÜNİVERSİTESİ, İSTANBUL TIP FAKÜLTESİ)0000-0002-9099-4779
Tuğba Kotil(İSTANBUL ÜNİVERSİTESİ, İSTANBUL TIP FAKÜLTESİ)0000-0003-1261-0597
Sayfa
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Yayın Tarihi
2026-10-08
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Mitochondrial changes may be important early cellular findings in PER-induced testicular damage.

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Amaç & Hipotez:Objective: The aim was to investigate the dose-dependent effects of PER on testes.
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Temel Bulgular & Veri:Material & Methods: Male Wistar albino rats (n=28; 2 months old; 250–400 g) were randomly divided into four groups (n = 7 in each group): control (sham), low (20 mg/kg/day), medium (40 mg/kg/day), and high (75 mg/kg/day) dose PER groups.
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Sonuç & Katkı:Mitochondrial changes may be important early cellular findings in PER-induced testicular damage.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Oxidative stress · Permethrin · Permethrin

Abstract

Objective: The aim was to investigate the dose-dependent effects of PER on testes. Material & Methods: Male Wistar albino rats (n=28; 2 months old; 250–400 g) were randomly divided into four groups (n = 7 in each group): control (sham), low (20 mg/kg/day), medium (40 mg/kg/day), and high (75 mg/kg/day) dose PER groups. PER was administered orally for 14 days. Testicular morphology was evaluated by hematoxylin-eosin staining. Immunohistochemically, superoxide dismutase 1 (SOD1), catalase (CAT), and active caspase-3 expression were examined. Ultrastructural intracellular alterations were examined using transmission electron microscopy. Results: Light microscopy showed that the general structure of the seminiferous tubules was largely preserved. In contrast, edema in the interstitial space and decreased cell density were observed in the medium and high dose groups. SOD1 expression was significantly increased in the high dose group, while CAT expression was significantly decreased in the medium and high dose groups. Active caspase-3 staining was dose-dependent and significantly increased in both the medium and high dose groups. Electron microscopic examination revealed mitochondrial swelling and cristae disruption, chromatin condensation, cytoplasmic vacuoles, and endoplasmic reticulum enlargement. Conclusion: The findings indicate that PER disrupts the antioxidant defense balance and increases apoptosis in testicular tissue. Mitochondrial changes may be important early cellular findings in PER-induced testicular damage.

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Bu Makaleye Atıf Yap

Yılmaz, T. E., Kotil, T. (2026). Effects of Permethrin on Oxidative Stress, Apoptosis, Mitochondrial Ultrastructure in Rat Testes. Balıkesir Sağlık Bilimleri Dergisi, Volume: 15 Issue: 3. https://doi.org/10.53424/balikesirsbd.1952109

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Kaynakça (28)

  1. Antinozzi, C., Di Luigi, L., Sireno, L., Caporossi, D., Dimauro, I. & Sgrò, P. (2025). Protective Role of Physical Activity and Antioxidant Systems During Spermatogenesis. Biomolecules, 15(4), 1–19. https://doi.org/10.3390/biom15040478
  2. Aoiadni, N., Chiab, N., Jdidi, H., Gargouri Bouzid, R., El Feki, A., Fetoui, H. & Ghorbel Koubaa, F. (2022). The pyrethroid insecticide permethrin confers hepatotoxicity through DNA damage and mitochondria-associated apoptosis induction in rat: Palliative benefits of Fumaria officinalis. Journal of Biochemical and Molecular Toxicology, 36(10). https://doi.org/10.1002/JBT.23172
  3. Aoiadni, N., Jdidi, H., Feki, A. El, Fetoui, H. & Koubaa, F. G. (2022). Mitochondrial bioenergetics and redox dysfunction in nephrotoxicity induced by pyrethroid permethrin are ameliorated by flavonoid-rich fraction. Environmental Science and Pollution Research International, 29(42), 63973–63987. https://doi.org/10.1007/S11356-022-20350-7
  4. Byun, K. A., Oh, S. M., Oh, S., Kim, H. M., Oh, M., Kim, G., Kim, M. S., Son, K. H. & Byun, K. (2025). Atelocollagen Increases Collagen Synthesis by Promoting Glycine Transporter 1 in Aged Mouse Skin. International Journal of Molecular Sciences, 26(24), 1–20. https://doi.org/10.3390/ijms262411825
  5. Cardoso, E. K. S., Kubota, K., Luz, D. A., Mendes, P. F. S., Figueiredo, P. L. B., Lima, R. R., Maia, C. S. F. & Fontes-Júnior, E. A. (2022). Aniba canelilla (Kunth) Mez (Lauraceae) Essential Oil: Effects on Oxidative Stress and Vascular Permeability. Antioxidants, 11(10), 1–13. https://doi.org/10.3390/antiox11101903
  6. Choi, S. Il, Kim, T. I., Kyu, S. K., Kim, B. Y., Ahn, S. Y., Cho, H. J., Hyung, K. L., Cho, H. S. & Eung, K. K. (2009). Decreased catalase expression and increased susceptibility to oxidative stress in primary cultured corneal fibroblasts from patients with granular corneal dystrophy type II. American Journal of Pathology, 175(1), 248–261. https://doi.org/10.2353/ajpath.2009.081001
  7. Ekiz-yilmaz, T., Isildar, B., Gezer, A. & Kankaya, D. (2021). The role of unfolded protein response in the pathogenesis of endometriosis: contribution of peritoneal fluid. Reproductive BioMedicine Online, 42(1), 1–15. https://doi.org/10.1016/j.rbmo.2020.09.012
  8. Elmore, S. (2007). Apoptosis: a review of programmed cell death. Toxicologic Pathology, 35(4), 495–516. https://doi.org/10.1080/01926230701320337
  9. França, L. R., Hess, R. A., Dufour, J. M., Hofmann, M. C. & Griswold, M. D. (2016). The Sertoli cell: one hundred fifty years of beauty and plasticity. Andrology, 4(2), 189–212. https://doi.org/10.1111/andr.12165
  10. Gautam, D. K., Misro, M. M., Chaki, S. P. & Sehgal, N. (2006). H 2O 2 at physiological concentrations modulates Leydig cell function inducing oxidative stress and apoptosis. Apoptosis, 11(1), 39–46. https://doi.org/10.1007/s10495-005-3087-1
  11. Green, D. R., Galluzzi, L. & Kroemer, G. (2011). Mitochondria and the autophagy-inflammation-cell death axis in organismal aging. Science (New York, N.Y.), 333(6046), 1109–1112. https://doi.org/10.1126/science.1201940
  12. Grzegorzewska, A. K., Wolak, D. & Hrabia, A. (2024). Effect of tamoxifen treatment on catalase (CAT) and superoxide dismutase (SOD) expression and localization in the hen oviduct. Theriogenology, 214(October 2023), 73–80. https://doi.org/10.1016/j.theriogenology.2023.10.008
  13. Ighodaro, O. M. & Akinloye, O. A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54(4), 287–293. https://doi.org/10.1016/j.ajme.2017.09.001
  14. Ismy, J., Sugandi, S., Rachmadi, D., Hardjowijoto, S. & Mustafa, A. (2020). The Effect of Exogenous Superoxide Dismutase (SOD) on Caspase-3 Activation and Apoptosis Induction in Pc-3 Prostate Cancer Cells. Research and Reports in Urology, 12, 503–508. https://doi.org/10.2147/RRU.S271203
  15. Jin, Y., Liu, J., Wang, L., Chen, R., Zhou, C., Yang, Y., Liu, W. & Fu, Z. (2012). Permethrin exposure during puberty has the potential to enantioselectively induce reproductive toxicity in mice. Environment International, 42(1), 144–151. https://doi.org/10.1016/j.envint.2011.05.020
  16. Kukucka, M. A. & Misra, H. P. (1993). The antioxidant defense system of isolated guinea pig Leydig cells. Molecular and Cellular Biochemistry, 126(1), 1–7. https://doi.org/10.1007/BF01772202
  17. Lee, H. S., Song, H. J., Park, Y., Smolensky, D. & Lee, S. H. (2022). Permethrin inhibits tube formation and viability of endothelial cells. Journal of the Science of Food and Agriculture, 102(10), 4079–4085. https://doi.org/10.1002/JSFA.11757
  18. López-Aceves, T. G., Coballase-Urrutia, E., Estrada-Rojo, F., Vanoye-Carlo, A., Carmona-Aparicio, L., Hernández, M. E., Pedraza-Chaverri, J., Navarro, L., Aparicio-Trejo, O. E., Pérez-Torres, A., Medina-Campos, O. N., Martínez-Fong, D., Sánchez-Valle, V., Cárdenas-Rodríguez, N., Granados-Rojas, L., Pulido-Camarillo, E., Rodríguez-Mata, V. & León-Sicairos, C. D. R. (2021). Exposure to Sub-Lethal Doses of Permethrin Is Associated with Neurotoxicity: Changes in Bioenergetics, Redox Markers, Neuroinflammation and Morphology. Toxics, 9(12). https://doi.org/10.3390/TOXICS9120337
  19. Mruk, D. D. & Cheng, C. Y. (2015). The Mammalian Blood-Testis Barrier: Its Biology and Regulation. Endocrine Reviews, 36(5), 564–591. https://doi.org/10.1210/er.2014-1101
  20. Oberoi, S., Ahmed, R. S., Suke, S. G., Nath Bhattacharya, S., Chakraborti, A. & Dev Banerjee, B. (2007). Comparative effect of topical application of lindane and permethrin on oxidative stress parameters in adult scabies patients. Clinical Biochemistry, 40(16–17), 1321–1324. https://doi.org/10.1016/j.clinbiochem.2007.07.011
  21. Personne, S., Brochot, C., Marcelo, P., Corona, A., Desmots, S., Robidel, F., Lecomte, A., Bach, V. & Zeman, F. (2021). Evaluation of Placental Transfer and Tissue Distribution of cis- and Trans-Permethrin in Pregnant Rats and Fetuses Using a Physiological-Based Pharmacokinetic Model. Frontiers in Pediatrics, 9. https://doi.org/10.3389/FPED.2021.730383
  22. Sharma, P., Kaushal, N., Saleth, L. R., Ghavami, S., Dhingra, S. & Kaur, P. (2023). Oxidative stress-induced apoptosis and autophagy: Balancing the contrary forces in spermatogenesis. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1869(6). https://doi.org/10.1016/j.bbadis.2023.166742
  23. Shrestha, S., Parks, C. G., Umbach, D. M., Hofmann, J. N., Beane Freeman, L. E., Blair, A. & Sandler, D. P. (2022). Use of permethrin and other pyrethroids and mortality in the Agricultural Health Study. Occupational and Environmental Medicine, 79(10), 664–672. https://doi.org/10.1136/OEMED-2021-108156
  24. Symington, S. B., Toltin, A. C., Murenzi, E., Lansky, D. & Clark, J. M. (2023). Determination of potential toxicodynamic differences of pyrethroid insecticides on native voltage-sensitive sodium channels in juvenile versus adult rat brain. Pesticide Biochemistry and Physiology, 189. https://doi.org/10.1016/j.pestbp.2022.105296
  25. Taghvaei, M. M., Lahiji, H. S. & Golfazani, M. M. (2022). Evaluation of expression changes, proteins interaction network, and microRNAs targeting catalase and superoxide dismutase genes under cold stress in rapeseed (Brassica napus L.). OCL, 29, 3. https://doi.org/10.1051/OCL/2021051
  26. Williams, M. T., Gutierrez, A. & Vorhees, C. V. (2018). Effects of Acute Exposure of Permethrin in Adult and Developing Sprague-Dawley Rats on Acoustic Startle Response and Brain and Plasma Concentrations. Toxicological Sciences, 165(2), 361–371. https://doi.org/10.1093/toxsci/kfy142
  27. Wu, P. Y., Scarlata, E. & O’flaherty, C. (2020). Long-term adverse effects of oxidative stress on rat epididymis and spermatozoa. Antioxidants, 9(2). https://doi.org/10.3390/antiox9020170
  28. Yenkoyan, K., Harutyunyan, H. & Harutyunyan, A. (2018). A certain role of SOD/CAT imbalance in pathogenesis of autism spectrum disorders. Free Radical Biology and Medicine, 123(February), 85–95. https://doi.org/10.1016/j.freeradbiomed.2018.05.070