KaydedilenlerDerginizi ekleyin

Dergiler / Animal Health Production and Hygiene / 2025 / Cilt: 14 Sayı: 2

Alanya İlçesindeki Sahipsiz Köpeklerde Canine Parvovirus Enfeksiyonunun Araştırılması

Investigation of Canine Parvovirus Infection in Stray Dogs in Alanya Region

Orhan Yapıcı(SELCUK UNIVERSITY)0000-0001-8778-3084
Irmak Dik(SELCUK UNIVERSITY)0000-0003-2516-9489
Sibel Yavru(SELCUK UNIVERSITY)0000-0002-5839-364X
Oğuzhan Avcı(SELCUK UNIVERSITY)0000-0001-9299-4695
Oya Bulut(DOKUZ EYLUL UNIVERSITY)0000-0002-2407-7390
Mehmet Kale(MEHMET AKIF ERSOY UNIVERSITY)0000-0003-4156-1077
Rüveyde Gülbahçe(SELCUK UNIVERSITY)0000-0002-3665-4642
Sayfa
1–8
Yayın Tarihi
2025-12-25
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sonuç olarak, bu çalışma okuyuculara Alanya bölgesindeki CPV enfeksiyonunun prevalansı, test güvenilirliği ve seroprevalansı hakkında bilgi sunarak, hastalığın kontrol altına alınmasına yönelik stratejilerin geliştirilmesi gerekliliğini vurgulamaktadır.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:Canine Parvovirus (CPV), evcil ve yabani köpekleri dünya genelinde etkileyen, özellikle yavru köpeklerde yüksek morbidite ve mortaliteye neden olan son derece bulaşıcı bir viral hastalıktır.
📊
Temel Bulgular & Veri:Bu çalışmada, 0-12 aylık CPV yönünden aşı uygulaması yapılmamış köpeklerden alınan dışkı örneklerinde CPV enfeksiyonunun varlığı, Enzyme-Linked ImmunoSorbent Assay (ELISA) ve hızlı test kiti (immunokromotografi (IC)) kullanılarak tespit edilip bu yaygın teşhis yöntemlerinin sonuçları karşılaştırıldı.
💡
Sonuç & Katkı:Sonuç olarak, bu çalışma okuyuculara Alanya bölgesindeki CPV enfeksiyonunun prevalansı, test güvenilirliği ve seroprevalansı hakkında bilgi sunarak, hastalığın kontrol altına alınmasına yönelik stratejilerin geliştirilmesi gerekliliğini vurgulamaktadır.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:CPV · immünite · sensitive

Özet

Canine Parvovirus (CPV), evcil ve yabani köpekleri dünya genelinde etkileyen, özellikle yavru köpeklerde yüksek morbidite ve mortaliteye neden olan son derece bulaşıcı bir viral hastalıktır. Virusun erken ve doğru teşhisi, ikincil enfeksiyonların azaltılması ve hastalığın duyarlı köpekler arasında yayılmasının önlenmesi açısından büyük önem taşımaktadır. Bu çalışmada, 0-12 aylık CPV yönünden aşı uygulaması yapılmamış köpeklerden alınan dışkı örneklerinde CPV enfeksiyonunun varlığı, Enzyme-Linked ImmunoSorbent Assay (ELISA) ve hızlı test kiti (immunokromotografi (IC)) kullanılarak tespit edilip bu yaygın teşhis yöntemlerinin sonuçları karşılaştırıldı. Ayrıca, kan serum örneklerinde CPV'ye karşı oluşan IgG antikorlarının seropozitifliği belirlenerek koruyucu antikor titreleri değerlendirildi. Toplam 100 köpeğin (%51 dişi, %49 erkek) 49'u (%49) hızlı test kiti ile CPV antijen pozitif bulunurken, ELISA Ag testi ile 17'sinde (%17) CPV varlığı tespit edildi. Hızlı teste kıyasla ELISA testi, yavru köpeklerde daha düşük oranda CPV tespiti yaparak, aşısız köpeklerde CPV belirlemede spesifik ve hassas bir test olarak tercih edilebileceği belirlendi. Ayrıca, ELISA antikor test kitindeki kesme değerine göre antikor pozitif olarak değerlendirilen 42 köpeğin 22'sinde (%52,4) yüksek, 20'sinde (%47,6) düşük CPV IgG antikor titreleri belirlenmiştir. İstatistiksel analizler, cinsiyet ile bağışıklık düzeyi arasında anlamlı bir fark olmadığını gösterdi (P > 0.001). Ancak, köpeklerin yaşı ile bağışıklık düzeyi arasında anlamlı bir ilişki bulundu (P < 0.001). Çalışma, Alanya bölgesindeki köpeklerde CPV’ye özgü IgG antikorlarının varlığını doğrulamıştır. Sonuç olarak, bu çalışma okuyuculara Alanya bölgesindeki CPV enfeksiyonunun prevalansı, test güvenilirliği ve seroprevalansı hakkında bilgi sunarak, hastalığın kontrol altına alınmasına yönelik stratejilerin geliştirilmesi gerekliliğini vurgulamaktadır.

Abstract

Canine Parvovirus (CPV) is a highly contagious viral disease that affects domestic and wild dogs worldwide, causing high morbidity and mortality, especially in puppies. Early and accurate diagnosis of the virus is crucial for reducing secondary infections and preventing the spread of the disease among susceptible dogs. In this study, the presence of CPV infection was detected in fecal samples collected from unvaccinated dogs aged 0–12 months using Enzyme-Linked ImmunoSorbent Assay (ELISA) and a rapid test kit (immunochromatographic (IC)), and the results of these common diagnostic methods were compared. Additionally, CPV IgG antibody seropositivity was investigated from blood serum samples to evaluate protective titers. Among 100 dogs (51% female, 49% male), 49 (49%) tested positive for CPV-2 Ag via the IC test kit, while 17 (17%) were positive using the ELISA Ag test. Compared to the rapid test, the ELISA test detected CPV at a lower rate in puppies, suggesting that it may be preferred as a specific and sensitive test for identifying CPV in unvaccinated dogs. Moreover, among 42 dogs considered antibody-positive based on ELISA kit cut-off values, 22 (52.4%) had high CPV IgG antibody titers, while 20 (47.6%) had low antibody titers. Statistical analyses showed no significant difference between gender and immunity levels (P > 0.001). However, a significant relationship was observed between the age of dogs and immunity levels (P < 0.001). The study confirmed the presence of CPV-specific IgG antibodies in dogs from the Alanya. In conclusion, this study has provided readers with information on the prevalence of CPV infection, test reliability, and seroprevalence in Alanya region, recommending the development of strategies for disease control.

🔖

Bu Makaleye Atıf Yap

Yapıcı, O., Dik, I., Yavru, S., Avcı, O., Bulut, O., Kale, M., Gülbahçe, R. (2025). Alanya İlçesindeki Sahipsiz Köpeklerde Canine Parvovirus Enfeksiyonunun Araştırılması. Animal Health Production and Hygiene, Cilt: 14 Sayı: 2, 1–8. https://doi.org/10.53913/aduveterinary.1665037

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (49)

  1. Ahmed Ismail, W.F., & Hanedan, B. (2024). Investigation of prevalence and risk factors of Parvovirus infection in dogs in Erzurum province, Turkey. Eurasian Journal of Veterinary Sciences, 40(1) 16-23. https://doi.org/10.15312/EurasianJVetSci.2024.421
  2. Babalola, E.T., Ijaopo, O.K., & Okonko, I.O. (2016). Evaluation of immunity and seropositivity of IgG antibodies to canine parvoviruses in vaccinated and unvaccinated dogs in Abeokuta, Nigeria. Journal of Immunoassay and Immunochemistry, 37(1), 16-28. https://doi.org/10.1080/15321819.2015.1040159
  3. Bagali, S., Kakhandaki, L., Karigoudar, R., Wavare, S., Shahapur, P.R., & Patil, M.M. (2023). Comparative analysis of enzyme-linked immunosorbent assay and immunochromatography for rotavirus and adenovirus detection in children below five years with acute gastroenteritis.Journal of Laboratory Physicians, 15(1), 110-116. https://doi.org/10.1055/s-0042-1757234
  4. Behera, M., Panda, S.K., Sahoo, P.K., Acharya, A.P., Patra, R.C., Das, S., & Pati, S. (2015). Epidemiological study of canine parvovirus infection in and around Bhubaneswar, Odisha, India. Veterinary World, 8(1), 33-37. https://doi.org/10.14202/vetworld.2015.33-37
  5. Böhm, M., Herrtage, M. E., Thompson, H., Weir, A., Hasted, A.M., & Maxwell, N.S. (2004). Serum antibody titres to canine parvovirus, adenovirus, and distemper virus in dogs in the UK which had not been vaccinated for at least three years. Veterinary Record, 154(15), 457-463. https://doi.org/10.1136/vr.154.15.457
  6. Dall’Ara, P., Lauzi, S., Zambarbieri, J., Servida, F., Barbieri, L., Rosenthal, R., … & Filipe, J. (2023). Prevalence of serum antibody titers against core vaccine antigens in Italian dogs. Life, 13(2), 587. https://doi.org/10.3390/life13020587
  7. Decaro, N., Campolo, M., Desario, C., Elia, G., Martella, V., Lorusso, E., & Buonavoglia, C. (2005). Maternally derived antibodies in pups and protection from canine parvovirus infection. Biologicals, 33(4), 261-267. https://doi.org/10.1016/j.biologicals.2005.06. 004
  8. Decaro, N., Desario, C., Beall, M.J., Cavalli, A., Campolo, M., DiMarco,A., … & Buonavoglia, C. (2010). Detection of canine parvovirus type 2c by a commercially available in-house rapid test. The Veterinary Journal, 184(3), 373-375. https://doi.org/10.1016/j.tvjl.2009.04.006
  9. Decaro, N., Buonavoglia, C., & Barrs, V. (2020). Canine parvovirus vaccination and immunisation failures: Are we far from disease eradication? Veterinary Microbiology, 247, 108760. https://doi.org/10.1016/j.vetmic.2020.108760
  10. Devajani Deka, D., Phukan, A., Sarma, D.K., Chakraborty, A.K., & Das, S. (2015). Canine parvovirus infection in immunized and nonimmunized diarrhoeic dogs. Indian Journal of Veterinary Pathology, 39, 125-128. https://doi.org/10.5958/0973-970X.2015.00029.2
  11. Dhiman, S., Devi, B., Singh, K., & Devi, P. (2015). Comparison of enzyme-linked immunosorbent assay and immunochromatography for rotavirus detection in children below five years with acute gastroenteritis. Journal of Clinical Diagnostical Research, 9(9), 6-9. https://doi.org/10.7860/JCDR/2015/14049.6425
  12. Dik, I., & Simsek, A. (2021). Comparison of different diagnostic methods in detection of canine parvovirus infection. Eurasian Journal of Veterinary Sciences, 37(2), 76-81. https://doi.org/10.15312/EurasianJVetSci.2021.329
  13. Dik, I., Aslım, H. P., & Bulut, O. (2023). Evaluation of the Performance of Diagnostic Methods of Canine Parvovirus-2 and Canine Enteric Coronavirus Infections under Different Storage Eurasian Journal of Veterinary Sciences, 39(4), 156-163. https://doi.org/10.15312/ EurasianJVetSci.2023.412
  14. Duque-García, Y., Echeverri-Zuluaga, M., Trejos-Suarez, J., & RuizSaenz, J. (2017). Prevalence and molecular epidemiology of canine parvovirus 2 in diarrheic dogs in Colombia, South America: A possible new CPV-2a is emerging? Veterinary Microbiology, 201, 56-61. https://doi.org/10.1016/j.vetmic.2016.12.039
  15. Eghafona, N.O., Jacob, J., & Yah, S.C. (2007). Evaluation of postvaccination immunity to canine distemper and parvoviruses in Benin City, Nigeria. African Journal of Biotechnology, 6(16). https://doi.org/10.5897/AJB2007.000-2286
  16. Elbaz, E., El-Tholoth, M., Elfadl, E.A.A., & Mosad, S.M. (2021). Molecular investigation on the presence of canine parvovirus in Egypt. Comparative Immunology, Microbiology and Infectious Diseases, 74, 101576. https://doi.org/10.1016/j.cimid.2020.101576
  17. Giraldo-Ramirez, S., Rendon-Marin, S., & Ruiz-Saenz, J. (2020). Phylogenetic, evolutionary, and structural analysis of canine parvovirus (CPV-2) antigenic variants circulating in Colombia. Viruses, 12(5), 500. https://doi.org/10.3390/v12050500
  18. Godsall, S.A., Clegg, S.R., Stavisky, J.H., Radford, A.D. & Pinchbeck G. (2010). Epidemiology of canine parvovirus and coronavirus in dogs presented with severe diarrhoea to PDSA PetAid hospitals. Veterinary Record, 167(6), 196-201. https://doi.org/10.1136/vr.c3095
  19. Gülbahçe, R., Palancı, H.S., Dik, I., Baş, T., & Bulut, O. (2025). Evaluation of Canine parvovirus–2 case observed in a Toy Poodle breed dog. Journal of Istanbul Veterinary Sciences, 9(1), 23-29. https://doi.org/10.30704/http-www-jivs-net.1600129
  20. Hao, X., He, Y., Wang, C., Xiao, W., Liu, R., Xiao, X., … & Li, S. (2020). The increasing prevalence of CPV2c in domestic dogs in China. PeerJ, 8, e9869. https://doi.org/10.7717/peerj.9869
  21. Ibrahim, S.B., El-Bialy, A.A., Mohammed, M.S., El-Sheikh, A.O., Elhewala, A., & Bahgat, S. (2015). Detection of rotavirus in children with acute gastroenteritis in Zagazig University Hospitals. Egyptian Electronic Physician, 7(5), 1227-1233. https://doi.org/10.14661/1227
  22. Khatri, R., Poonam, M.H., & Minakshi, P. (2017). Epidemiology, pathogenesis, diagnosis and treatment of canine parvovirus disease in dogs: A mini review abstract. Journal of Veterinary Science & Medical Diagnosis, 6(3). https://doi.org/10.4172/2325-9590.1000233
  23. Kumari, G.D., Pushpa, R.R., Subramanyam, K.V., Rao, T.S., & Satheesh, K. (2022). Seroprevalence of parvovirus infections from vaccinated and unvaccinated dogs of Andhra Pradesh. Qeios. https://doi.org/10.32388/OVF6LF
  24. Lambe, U., Guray, M., Bansal, N., Kumar, P., Joshi, V.G., Khatri, R., …& Prasad, G. (2016). Canine parvovirus—An insight into diagnostic aspect. Journal of Experimental Biology, 4(3S), 23-30. http://dx.doi.org/10.18006/2016.4(3S).279.290
  25. Larson, H., de Figueiredo, A., Karafillakis, E., & Rawal, M. (2018). State of vaccine confidence in the EU 2018. Luxembourg: Publications Office of the European Union, 10(2018), 241099. https://doi.org/10.2875/241099
  26. Lechner, E.S., Crawford, P.C., Levy, J.K., Edinboro, C.H., Dubovi, E.J., & Caligiuri, R. (2010). Prevalence of protective antibody titers for canine distemper virus and canine parvovirus in dogs entering a Florida animal shelter. Journal of the American Veterinary Medical Association, 236(12), 1317-1321. https://doi.org/10.2460/javma.236.12.1317
  27. Litster, A., Pressler, B., Volpe, A., & Dubovi, E. (2012). Accuracy of a point-of-care ELISA test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs. The Veterinary Journal, 193(2), 363-366. https://doi.org/10.1016/j.tvjl.2012.01.027
  28. Macêdo, J.V., Frias, I.A., Oliveira, M.D., Zanghelini, F., & Andrade, C.A. (2022). A systematic review and etaanalysis on the accuracy of rapid immunochromatographic tests for dengue diagnosis. European Journal of Clinical Microbiology & Infectious Diseases, 41(9), 1191-1201. https://doi.org/10.1007/s10096-022-04485-6
  29. Mila, H., Grellet, A., Desario, C., Feugier, A., Decaro, N., Buonavoglia, C., & Chastant-Maillard, S. (2014). Protection against canine parvovirus type 2 infection in puppies by colostrum-derived antibodies. Journal Yapıcı et al CPV Detection by IC and ELISA 8 of Nutritional Science, 3, e54. https://doi.org/10.1017/jns.2014.57
  30. Miranda, C., Carvalheira, J., Parrish, C.R., & Thompson, G. (2015). Factors affecting the occurrence of canine parvovirus in dogs. Veterinary Microbiology, 180(1-2), 59-64. https://doi.org/10.1016/j.vetmic.2015.08.002
  31. Nandi, S., & Kumar, M. (2010). Canine parvovirus: Current perspective. Indian Journal of Virology, 21(1), 31-44. https://doi.org/10.1007/s13337-010-0007-y
  32. Nandi, S., Sharma, G.K., Gupta, V., Deol, P., Chander, V., De, U., & Gupta, V. (2019). Global scenario of canine Parvovirus mutants: epidemiology, diagnostics and immunoprophylactic agents. JSM Veterinary Medicine and Research, 2, 12.
  33. Ogbu, K., Anene, B., Nweze, N., Okoro, J., Danladi, M., & Ochai, S. (2017). Canine parvovirus: A review. Journal of Veterinary and Animal Sciences, 44(1), 1-10
  34. Riedl, M., Truyen, U., Reese, S., & Hartmann, K. (2015). Prevalence of antibodies to canine parvovirus and reaction to vaccination in client‐owned, healthy dogs. Veterinary Record, 177(23), 597-599. https://doi.org/10.1136/vr.103271
  35. Rota, A., Dogliero, A., Muratore, E., Pregel, P., Del Carro, A., & Masoero, L. (2019). Serological survey of canine parvovirus 2 antibody titres in breeding kennels in northern Italy. BMC Veterinary Research, 15, 1-5.
  36. Schultz, R.D., Thiel, B., Mukhtar, E., Sharp, P., & Larson, L.J. (2010). Age and long-term protective immunity in dogs and cats. Journal of Comparative Pathology, 142(1), S102–S108. https://doi.org/10.1016/j.jcpa.2009.10.009
  37. Sharma, C., Singh, M., Upmanyu, V., Chander, V., Verma, S., Chakrovarty, S., … & Gupta, V. K. (2018). Development and evaluation of a gold nanoparticle-based immunochromatographic strip test for the detection of canine parvovirus. Archives of Virology, 163, 2359-2368. https://doi.org/ 10.1007/s00705-018-3846-2
  38. Suzuki, K., Huits, R., Phadungsombat, J., Tuekprakhon, A., Nakayama, E.E., Van Den Berg, R., & Shioda, T. (2020). Promising application of monoclonal antibody against chikungunya virus E1-antigen across genotypes in immunochromatographic rapid diagnostic tests. Virology Journal, 17, 1-11. https://doi.org/10.1186/s12985-020-01364-4
  39. Tagorti, G. (2018). Prevalence of canine parvovirus infection in Grand Tunis, Tunisia. Journal of Advanced Veterinary and Animal Research, 5(1), 93-97. https://doi.org/10.5455/javar.2018.e251
  40. Temizkan, M.C., & Temizkan, S. (2023). Canine parvovirus in Turkey: first whole-genome sequences, strain distribution, and prevalence. Viruses, 15(4), 957. https://doi.org/10.3390/v15040957
  41. Topcu, M., Köroglu, M., Aydemir, O., Aydemir, Y., Altındis, M., & Elmas, B. (2023). Investigation of Viruses Causing Gastroenteritis by Immunochromatographic Tests and Multiplex PZR Method. Value in Health Sciences, 13(1), 1-5. https://doi.org/10.33631/sabd.1073789
  42. Tuteja, D., Banu, K., & Mondal, B. (2022). Canine parvovirology–A brief updated review on structural biology, occurrence, pathogenesis, clinical diagnosis, treatment and prevention. Comparative Immunology, Microbiology and Infectious Diseases, 82, 101765. https://doi.org/10.1016/j.cimid.2022.101765
  43. Twark, L., & Dodds, W.J. (2000). Clinical use of serum parvovirus and distemper virus antibody titers for determining revaccination strategies in healthy dogs. Journal of the American Veterinary Medical Association, 217(7), 1021-1024. https://doi.org/10.2460/javma.2000.217.1021
  44. Walter-Weingärtner, J., Bergmann, M., Weber, K., Truyen, U., Muresan, C., & Hartmann, K. (2021). Comparison of eight commercially available faecal point-of-care tests for detection of canine parvovirus antigen. Viruses, 13(10), 2080. https://doi.org/10.3390/v13102080
  45. Waner, T., Naveh, A., Wudovsky, I., & Carmichael, L.E. (1996). Assessment of maternal antibody decay and response to canine parvovirus vaccination using a clinic-based enzyme-linked immunosorbent assay. Journal of Veterinary Diagnostic Investigation, 8, 427 -432
  46. Yang, D.K., Yoon, S.S., Byun, J.W., Lee, K.W., Oh, Y.I., & Song, J.Y. (2010). Serological survey for canine parvovirus type 2a (CPV-2a) in the stray dogs in South Korea. Journal of Bacteriology and Virology, 40(2), 77-81. https://doi.org/10.4167/jbv.2010.40.2.77
  47. Yılmaz, V. (2020). Virological survey of canine parvovirus infection in Kars shepherd dogs in Kars Province, Turkey. Advances in Animal and Veterinary Sciences, 8(7), 687-691. https://doi.org/10.17582/journal.aavs/2020/8.7.687.691
  48. Yılmaz, Z., Pratelli, A., & Torun, S. (2005). Distribution of antigen types of canine parvovirus type 2 in dogs with hemorrhagic enteritis in Turkey. Turkish Journal of Veterinary and Animal Sciences, 29(4), 1073-1076
  49. Bagali, S., Kakhandaki, L., Karigoudar, R., Wavare, S., Shahapur, P.R., & Patil, M.M. (2023). Comparative analysis of enzyme-linked immunosorbent assay and immunochromatography for rotavirus and adenovirus detection in children below five years with acute gastroenteritis.Journal of Laboratory Physicians, 15(1), 110-116. https://doi.org/10.1055/s-0042-1757234