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Dergiler / Animal Health Production and Hygiene / 2026 / Cilt: 15 Sayı: 3

Safranal; Kan, Karaciğer, Böbrek ve Beyin Matrislerinde Malation Kaynaklı Oksidatif Stresi ve AChE İnhibisyonunu Hafifletir

Safranal Attenuates Malathion-Induced Oxidative Stress and AChE Inhibition Across Blood, Hepatic, Renal, and Brain Matrices

Ahmet Büyükben(AFYON KOCATEPE UNIVERSITY, ÇAY VOCATIONAL SCHOOL)0000-0002-5639-7217
Ömer Hazman(AFYON KOCATEPE UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0000-0002-2702-6847
Laçine Aksoy(AFYON KOCATEPE UNIVERSITY, FACULTY OF ARTS AND SCIENCES)0000-0001-8086-5079
Funda Karabağ(USAK UNIVERSITY)0000-0002-1565-3210
Sayfa
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Yayın Tarihi
2026-08-23
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular, malation maruziyetinin; şiddetli AChE inhibisyonu, hızlanmış lipid peroksidasyonu, yükselmiş NO seviyeleri ve analiz edilen tüm matrislerde hem enzimatik hem de enzimatik olmayan antioksidan savunma hatlarında kritik bir tükenme ile karakterize derin bir toksisiteye yol açtığını göstermiştir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Başta malation olmak üzere organofosfatlı pestisitler tarımda yaygın olarak kullanılmakta, ancak asetilkolinesteraz (AChE) enzim inhibisyonu ve oksidatif stresi tetikleme mekanizmalarıyla hedef olmayan organizmalar üzerinde ciddi toksisite riskleri oluşturmaktadır.
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Temel Bulgular & Veri:Bu çalışmada, safranın (Crocus sativus L.) antioksidan ve anti-enflamatuar özellikleri ile bilinen temel biyoaktif bileşeni safranalın, malation kaynaklı sistemik ve dokuya özgü toksisiteye karşı olası koruyucu etkileri araştırılmıştır.
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Sonuç & Katkı:Bulgular, malation maruziyetinin; şiddetli AChE inhibisyonu, hızlanmış lipid peroksidasyonu, yükselmiş NO seviyeleri ve analiz edilen tüm matrislerde hem enzimatik hem de enzimatik olmayan antioksidan savunma hatlarında kritik bir tükenme ile karakterize derin bir toksisiteye yol açtığını göstermiştir.
🔬Tür:Laboratuvar & Saha Deneyi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Safranal · Organofosfatlar · Malatyon

Özet

Başta malation olmak üzere organofosfatlı pestisitler tarımda yaygın olarak kullanılmakta, ancak asetilkolinesteraz (AChE) enzim inhibisyonu ve oksidatif stresi tetikleme mekanizmalarıyla hedef olmayan organizmalar üzerinde ciddi toksisite riskleri oluşturmaktadır. Bu çalışmada, safranın (Crocus sativus L.) antioksidan ve anti-enflamatuar özellikleri ile bilinen temel biyoaktif bileşeni safranalın, malation kaynaklı sistemik ve dokuya özgü toksisiteye karşı olası koruyucu etkileri araştırılmıştır. Deneysel gruplar; kan, karaciğer, böbrek ve beyin dokularında malondialdehit (MDA), nitrik oksit (NO), glutatyon (GSH), süperoksit dismütaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GPx) düzeylerinin yanı sıra AChE enzim aktivitesi ölçülerek değerlendirilmiştir. Bulgular, malation maruziyetinin; şiddetli AChE inhibisyonu, hızlanmış lipid peroksidasyonu, yükselmiş NO seviyeleri ve analiz edilen tüm matrislerde hem enzimatik hem de enzimatik olmayan antioksidan savunma hatlarında kritik bir tükenme ile karakterize derin bir toksisiteye yol açtığını göstermiştir. Buna karşılık, safranal uygulaması etkili bir terapötik müdahale sağlamıştır. Safranal oksidatif hasarı hafifletmiş, reaktif oksijen türlerini azaltarak temel antioksidan enzimlerin tükenmesini sınırlandırmış ve AChE fonksiyonelliğinin korunmasını desteklemiştir. Özellikle, safranalın koruyucu etkinliği dokuya bağlı varyasyonlar göstermiştir; plazma, karaciğer ve böbrek matrislerinde oksidatif stres belirteçlerini önemli ölçüde azaltıp hücresel canlılığı korurken, mevcut deneysel koşullar altında beyin dokusunda sınırlı bir koruyucu kapasite sergilemiştir. Sonuç olarak safranal, organofosfat kaynaklı sistemik hasara karşı gelecek vaat eden biyoaktif bir önlem olarak öne çıkmaktadır. Bununla birlikte, kan-beyin bariyerini geçmedeki kesin taşınma mekanizmalarını ve olası sınırlılıklarını aydınlatmak için daha fazla araştırmaya ihtiyaç duyulmaktadır.

Abstract

Organophosphate pesticides, particularly malathion, are widely used in agriculture but pose severe toxicity risks to non-target organisms through acetylcholinesterase (AChE) enzyme inhibition and the induction of oxidative stress. This study investigated the potential protective effects of safranal, a primary bioactive component of saffron (Crocus sativus L.) known for its antioxidant and anti-inflammatory properties, against malathion-induced systemic and tissue-specific toxicity. Experimental groups were evaluated by measuring malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and GPx (glutathione peroxidase) levels, alongside AChE enzyme activity in the blood, liver, kidney, and brain tissues. The findings demonstrated that malathion exposure caused profound toxicity characterized by severe AChE inhibition, accelerated lipid peroxidation, elevated NO levels, and a critical depletion of both enzymatic and non-enzymatic antioxidant defenses across all analyzed matrices. Conversely, safranal administration served as an effective therapeutic intervention. It mitigated oxidative damage, limited the exhaustion of key antioxidant enzymes by reducing reactive oxygen species, and supported the preservation of AChE functionality. Notably, safranal’s protective efficacy exhibited tissue-dependent variations; while it significantly attenuated oxidative stress markers and preserved cellular viability in the plasma, liver, and renal matrices, it demonstrated limited protective capacity in brain tissue under the current experimental conditions. In conclusion, safranal represents a promising bioactive countermeasure against organophosphate-induced systemic injury. However, further research is warranted to elucidate its precise transport mechanisms and potential limitations in crossing the blood-brain barrier.

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Bu Makaleye Atıf Yap

Büyükben, A., Hazman, Ö., Aksoy, L., Karabağ, F. (2026). Safranal; Kan, Karaciğer, Böbrek ve Beyin Matrislerinde Malation Kaynaklı Oksidatif Stresi ve AChE İnhibisyonunu Hafifletir. Animal Health Production and Hygiene, Cilt: 15 Sayı: 3. https://doi.org/10.53913/aduveterinary.1963518

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