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Dergiler / Clinical Dentistry and Research / 2017 / Cilt: 41 - Sayı: 3

EFFECT OF NICOTINE ON PERIODONTAL LIGAMENT DERIVED CELLS CULTURED UNDER MECHANICAL STRESS

MEKANİK STRESS ALTINDA KÜLTÜRE EDİLEN PERİODONTAL LİGAMENT KAYNAKLI HÜCRELERDE NİKOTİNİN ETKİLERİ

Yuki IİJİMA
Taiki MORİKAWA
Kenichi MATSUZAKA
Kenji SUEİSHİ
Takashi INOUE
Sayfa
106–113
DOI
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Conclusion: Nicotine suppressed expression of OPG and accelerated osteoclastogenesis in PDL-derived cells under centrifugal force.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Background and Aim: The purpose of this study was to investigate the effects of nicotine on periodontal ligament (PDL) cells cultured under mechanical stress.
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Temel Bulgular & Veri:Materials And Methods: Periodontal ligament-derived cells obtained from rat incisors were cultured with nicotine (20 ng/ml, NI), under centrifugal force (2000 rpm/20 min, MS) as experimental group (NI+MS).
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Sonuç & Katkı:Conclusion: Nicotine suppressed expression of OPG and accelerated osteoclastogenesis in PDL-derived cells under centrifugal force.
🔬Tür:Araştırma Makalesi
📍Kapsam:2000 Verileri
⚙️Değişkenler:Alan Parametreleri

Özet

Background and Aim: The purpose of this study was to investigate the effects of nicotine on periodontal ligament (PDL) cells cultured under mechanical stress. Materials And Methods: Periodontal ligament-derived cells obtained from rat incisors were cultured with nicotine (20 ng/ml, NI), under centrifugal force (2000 rpm/20 min, MS) as experimental group (NI+MS). The control groups were treated with NI alone (NI), under MS without NI (MS), and without any treatment (CONT). An immunofluorescence study using an anti-receptor activator of nuclear factor-kappa B ligand (RANKL) primary antibody was also performed. The mRNA levels of RANKL, osteoprotegerin (OPG), interleukin-6 (IL-6), and vascular endothelial growth factor (VEGF) were analyzed using quantitative real-time polymerase chain reaction at day 1 of culture. Results: RANKL positive cells and RANKL mRNA expression in the NI+MS group, NI group, and MS group were significantly higher than that of the CONT group. Expression of RANKL mRNA of the NI+MS group was higher than that of the CONT group and NI group. Expression of OPG mRNA in the MS group was significantly higher than that of other 3 groups. Expression of IL-6 and VEGF mRNA in the NI+MS group, NI group and MS group were significantly higher than that of the CONT group. Conclusion: Nicotine suppressed expression of OPG and accelerated osteoclastogenesis in PDL-derived cells under centrifugal force.

Abstract

Amaç: Bu çalışmanın amacı mekanik stress altında kültüre edilen periodontal ligament hücrelerinde (PDL) nikotinin etkilerini araştırmaktır. Gereç ve yöntemler: Fare kesici dişlerinden elde edilen periodontal kaynaklı hücreler, deney grubu olarak (NI+MS) nikotin ile (20 ng/ml, NI) santrifüjel kuvvet altında (2000 rpm/20 dk, MS) kültüre edilmişlerdir. Kontrol grupları, sadece NI (NI), MS altında NI içermeyen (MS), ve herhangi bir işlem uygulanmamış (CONT) olarak ayrılmıştır. Nüklear factor-kappa B ligand (RANKL) primer antikorunun antireseptör aktivatörü kullanılarak immünoflorasan bir çalışma yapılmıştır. RANKL, osteoprotegerin (OPG), interlökin-6 (IL-6), ve vasküler endotelyal büyüme faktörü (VEGF) mRNA seviyeleri kuantitatif real-time polimeraz zincir reaksiyonu kullanilarak 1 günlük kültürde analiz edilmiştir. Bulgular: RANKL pozitif hücreler ve NI+MS, NI ve MS gruplarındaki mRNA salınımı kontrol grubundan belirgin bir şekilde daha yüksek bulunmuştur. NI+MS grubundaki RANKL mRNA salınımı CONT ve NI gruplarından daha yüksek bulunmuştur. MS grubundaki OPG mRNA salınımı diğer üç gruptan belirgin bir şekilde yüksektir. NI+MS, NI ve MS gruplarındaki VEGF ve IL-6 mRNA salınımı CONT grubundan belirgin bir şekilde yüksektir. Sonuçlar: Nikotin, santrifüjel kuvvet altındaki PDL-kaynaklı hücrelerdeki OPG salınımını baskılamış ve osteoklastogenezisi hızlandırmıştır

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Bu Makaleye Atıf Yap

IİJİMA, Y., MORİKAWA, T., MATSUZAKA, K., SUEİSHİ, K., INOUE, T. (2017). EFFECT OF NICOTINE ON PERIODONTAL LIGAMENT DERIVED CELLS CULTURED UNDER MECHANICAL STRESS. Clinical Dentistry and Research, Cilt: 41 - Sayı: 3, 106–113. https://ajindex.com/articles/144858

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Kaynakça (11)

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