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Dergiler / Adıyaman Üniversitesi Sağlık Bilimleri Dergisi / 2023 / Cilt: 9 Sayı: 3

Oksipösedanin kaynaklı antikanser aktivitenin in siliko ve in vitro değerlendirilmesi: Mitotoksisite?

In silico and in vitro evaluation of oxypeucedanin-induced anticancer activity: Mitotoxicity?

Ali Ergüç(İzmir Kâtip Çelebi Üniversitesi)0000-0002-9791-4399
Hayati Okur(DİCLE ÜNİVERSİTESİ)0000-0001-6807-8208
Fuat Karakuş(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ)0000-0002-5260-3650
Gökay Albayrak(İZMİR KATİP ÇELEBİ ÜNİVERSİTESİ)0000-0002-5729-0796
Ege Arzuk(EGE ÜNİVERSİTESİ)0000-0002-3239-4855
Şüra Baykan(EGE ÜNİVERSİTESİ)0000-0002-3624-4811
Sayfa
153–161
Yayın Tarihi
2023-12-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: Glukoz içeren ortamda 250 µM OKS canlılığı azaltmıştır.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Çalışmanın amacı, farklı ortamlarda Oksipösedanin (OKS) aracılı antikanser aktivitedeki değişiklikleri değerlendirmektir.
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Temel Bulgular & Veri:Bulgular: Glukoz içeren ortamda 250 µM OKS canlılığı azaltmıştır.
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Sonuç & Katkı:Ayrıca OKS, Kompleks I ve IV'ün seçici inhibisyonu nedeni ile ETZ disfonksiyonuna neden olabilmektedir.
🔬Tür:Laboratuvar & Saha Deneyi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Oksipösedanin · Mitotoksisite · Antikanser aktivite

Özet

Amaç: Çalışmanın amacı, farklı ortamlarda Oksipösedanin (OKS) aracılı antikanser aktivitedeki değişiklikleri değerlendirmektir. İkinci amaç, OKS’inin elektron transfer zincirine (ETZ) karşı afinitesini öngörmektir. Gereç ve Yöntem: MTT ve LDH sızma deneyleri OKS ile gerçekleştirilmiştir. Ayrıca, OKS’inin ETZ komplekslerine karşı afinitesini öngörmek için moleküler kenetlenme çalışmaları uygulanmıştır. Bulgular: Glukoz içeren ortamda 250 µM OKS canlılığı azaltmıştır. Galaktoz içeren ortamda ≥50 µM OKS hücre canlılığını azalmıştır. Galaktoz içeren ortamda ≥50 µM OKS membran parçalanmasını artırmıştır. Moleküler kenetlenme çalışmaları, OKS'inin Kompleks I ve IV'ün inhibisyon bölgelerine bağlanma kapasitesine sahip olabileceğini göstermektedir. Sonuç: Galaktoz içeren ortam, OKS aracılı sitotoksisiteyi artırmıştır. Ön sonuçlar, antikanser aktivitede mitotoksisitenin yer alabileceğini göstermektedir. Ayrıca OKS, Kompleks I ve IV'ün seçici inhibisyonu nedeni ile ETZ disfonksiyonuna neden olabilmektedir.

Abstract

Aim: This study aims to evaluate the alterations in Oxypeucedanin (OXY)-mediated anticancer activity in different media. Second aim is to predict the affinity of OXY to electron transfer chain (ETC) complexes. Materials and Methods: MTT and LDH leakage assays were performed with OXY. Molecular docking studies were also conducted to predict the affinity of OXY to ETC complexes. Results: 250 µM OXY reduced viability in glucose media. ≥50 µM OXY decreased viability in galactose media. ≥50 µM OXY increased membrane disruption in galactose media. Molecular docking studies also showed that OXY might possess the capacity to bind to the inhibition sites of Complex I and IV. Conclusion: Galactose-conditioned media exacerbated the OXY-mediated cytotoxicity. Preliminary results suggested that mitotoxicity might take part in anticancer activity. Furthermore, OXY might cause ETC dysfunctions due to selective inhibition of Complex I and IV.

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Bu Makaleye Atıf Yap

Ergüç, A., Okur, H., Karakuş, F., Albayrak, G., Arzuk, E., Baykan, Ş. (2023). Oksipösedanin kaynaklı antikanser aktivitenin in siliko ve in vitro değerlendirilmesi: Mitotoksisite?. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 9 Sayı: 3, 153–161. https://doi.org/10.30569/adiyamansaglik.1325975

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