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Dergiler / Journal of advances in vetbio science and techniques / 2026 / Issue: 2

From Traditional Infusion to Antioxidant Potential: Comparative Evaluation of Water, Methanol, and Acetone Extracts of Sideritis akmanii

Ulaş Acaröz(Afyon Kocatepe Üniversitesi)0000-0002-1533-4519
Ali Soylu(AFYON KOCATEPE UNIVERSITY)0000-0003-3199-782X
Damla Arslan-Acaroz(AFYON KOCATEPE UNIVERSITY)0000-0001-9230-6725
Sayfa
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Yayın Tarihi
2026-07-13
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Overall, the findings indicate that S. akmanii is a promising natural source of antioxidants, with methanol extracts showing the highest in vitro activity, and support further phytochemical and pharmacological investigation of this endemic species.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Herbal teas are widely consumed worldwide and represent an important component of the traditional diet in Türkiye.
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Temel Bulgular & Veri:Total phenolic content (TPC) was determined by the Folin–Ciocalteu method, antioxidant capacity (AC) by the phosphomolybdenum assay, and radical-scavenging activity by the DPPH test at 25, 50 and 100 ppm.
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Sonuç & Katkı:Overall, the findings indicate that S. akmanii is a promising natural source of antioxidants, with methanol extracts showing the highest in vitro activity, and support further phytochemical and pharmacological investigation of this endemic species.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Antioxidant capacity · herbal tea · phenolic content

Abstract

Herbal teas are widely consumed worldwide and represent an important component of the traditional diet in Türkiye. Sideritis akmanii, an endemic species used locally as a herbal infusion, has been scarcely characterized in terms of its phenolic composition and antioxidant properties. This study comparatively evaluated water, methanol and acetone extracts of S. akmanii prepared to mimic traditional use (water) and laboratory solvent extraction (methanol, acetone). Total phenolic content (TPC) was determined by the Folin–Ciocalteu method, antioxidant capacity (AC) by the phosphomolybdenum assay, and radical-scavenging activity by the DPPH test at 25, 50 and 100 ppm. Methanol yielded the highest TPC (41.31 ± 3.65 mg GAE/g), followed by acetone (32.41 ± 2.48 mg GAE/g) and water (28.81 ± 2.27 mg GAE/g), and showed the greatest AC (123.76 ± 4.58 μmol AAE/g for methanol vs. 97.14 ± 5.68 and 67.69 ± 2.17 μmol AAE/g for acetone and water, respectively). DPPH inhibition increased in a dose-dependent manner for all extracts, with methanol achieving up to 31.87 ± 2.86% inhibition at 100 ppm, acetone 24.54 ± 3.88%, and water 14.02 ± 1.27%. Radar plots and Pearson correlation analysis revealed uniformly strong positive associations among TPC, AC and DPPH responses (r = 0.90–1.00), while linear regression confirmed TPC as a major predictor of AC (R² = 0.929). Overall, the findings indicate that S. akmanii is a promising natural source of antioxidants, with methanol extracts showing the highest in vitro activity, and support further phytochemical and pharmacological investigation of this endemic species.

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Bu Makaleye Atıf Yap

Acaröz, U., Soylu, A., Arslan-Acaroz, D. (2026). From Traditional Infusion to Antioxidant Potential: Comparative Evaluation of Water, Methanol, and Acetone Extracts of Sideritis akmanii. Journal of advances in vetbio science and techniques, Issue: 2. https://doi.org/10.31797/vetbio.1837206

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