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Dergiler / Journal of advances in vetbio science and techniques / 2026 / Issue: 2

Effect of caffeic acid on freezing of epididymal sperm in domestic cat

Taygun Gökdemir(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0001-6049-7184
Ersin Tanrıverdi(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, HASTALIKLAR VE KLİNİK BİLİMLERİ BÖLÜMÜ, CERRAHİ ANABİLİM DALI)0000-0001-8502-2070
Muhammet Can Kılıç(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0003-0771-3970
Oğuzhan Şahin(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, HASTALIKLAR VE KLİNİK BİLİMLERİ BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0003-3123-8540
Alican Yılmaz(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, HASTALIKLAR VE KLİNİK BİLİMLERİ BÖLÜMÜ, CERRAHİ ANABİLİM DALI)0000-0001-7042-1749
Savaş Yıldız(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0001-6459-6841
Celal Şahin Ermutlu(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, HASTALIKLAR VE KLİNİK BİLİMLERİ BÖLÜMÜ, CERRAHİ ANABİLİM DALI)0000-0002-8923-7682
Nail Tekin Önder(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0001-5141-0008
Uğur Aydın(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, HASTALIKLAR VE KLİNİK BİLİMLERİ BÖLÜMÜ, CERRAHİ ANABİLİM DALI)0000-0001-5756-4841
Yavuz Öztürkler(KAFKAS ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK BİLİMLER BÖLÜMÜ, DÖLERME VE SUNİ TOHUMLAMA ANABİLİM DALI)0000-0002-0681-9311
Sayfa
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Yayın Tarihi
2026-05-07
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It is concluded that caffeic acid supports spermatological parameters but future applications are needed to establish a standard freezing protocol.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The aim of this study was to investigate the effect of caffeic acid on the freezing of epididymal cat sperm.
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Temel Bulgular & Veri:After castration, cauda epididymis were obtained from 10 cat testes.
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Sonuç & Katkı:It is concluded that caffeic acid supports spermatological parameters but future applications are needed to establish a standard freezing protocol.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Caffeic acid · domestic cat · freezing

Abstract

The aim of this study was to investigate the effect of caffeic acid on the freezing of epididymal cat sperm. After castration, cauda epididymis were obtained from 10 cat testes. Epidididymal spermatozoa were analysed for motility, viability, and concentration were evaluated and mixed. The mixed sperm was diluated with containing different amounts of caffeic acid (0.003 mg/mL (A1), 0.006 mg/mL (A2) and 0.010 mg/mL (A3)) and without caffeic acid (control). The groups were cryopreservation in liquid nitrogen vapour and the samples were thawed at 37°C for 30 seconds. After thawing, motility, viability, abnormal spermatozoa ratio, and HOS test were evaluated. As a result of the evaluation, although the caffeic acid groups were numerically higher than the control, they were not statistically different (P>0.05). It is concluded that caffeic acid supports spermatological parameters but future applications are needed to establish a standard freezing protocol.

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Bu Makaleye Atıf Yap

Gökdemir, T., Tanrıverdi, E., Kılıç, M. C., Şahin, O., Yılmaz, A., Yıldız, S., Ermutlu, C. Ş., Önder, N. T., Aydın, U., Öztürkler, Y. (2026). Effect of caffeic acid on freezing of epididymal sperm in domestic cat. Journal of advances in vetbio science and techniques, Issue: 2. https://doi.org/10.31797/vetbio.1749776

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Kaynakça (28)

  1. Alcay, S., Toker, M.B., Gokce, E., Ustuner, B., Onder, N.T., Sagırkaya, H., Nur, Z., & Soylu, M.K. (2015). Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology, 71: 329-333. https://doi.org/10.1016/j.cryobiol.2015.08.008
  2. Amstislavsky, S., Lindeberg, H., & Luvoni, G. C. (2012). Reproductive technologies relevant to the genome resource bank in carnivora. Reproduction Domestic Animals, 47(1), 164-175. https://doi.org/10.1111/j.1439-0531.2011.01886.x
  3. Axnér, E., & Forsberg, C. (2007). Sperm morphology in the domestic cat and its relation with fertility: A retrospective study. Reproduction Domestic Animals, 291(3), 282–291. https://doi.org/10.1111/j.1439-0531.2007.00780.x
  4. Buranaamnuay, K. (2017). Protocols for sperm cryopreservation in the domestic cat: A review. Animal Reproduction Science. 183, 56-65. https://doi.org/10.1016/j.anireprosci.2017.06.002
  5. Ceylan, T., Kaymak, E., Cantürk, F., & Yakan, B. (2020). Research on the protective effect of caffeic acid phenethyl ester on testicular damage caused by cisplatin. Turkish Journal of Medical Sciences, 50, 2032-2039. https://doi.org/10.3906/sag-2002-58
  6. Cheuquemán, C., Faúndez, R., Sánchez, R., & Risopatrón, J. (2018). Changes in sperm function and structure after freezing in domestic cat spermatozoa. Andrologia, 50(9), 13080. https://doi.org/10.1111/and.13080
  7. Cocchia, N., Ciani, F., El-Rass, R., Russo, M., Borzacchiello, G., Esposito, V., Montagnaro, S., Avallone, L., Tortora, G., & Lorizio, R. (2010). Cryopreservation of feline epididymal spermatozoa from dead and alive animals and its use in assisted reproduction. Zygote, 18(1), 1–8. https://doi.org/10.1017/S0967199409990256
  8. Driscoll, C. A., Clutton-Brock, J., Kitchener, A. C., & O'Brien, S. J. (2009). The Taming of the cat. Genetic and archaeological findings hint that wildcats became housecats earlier--and in a different place--than previously thought. Scirntific American, 300(6), 268-75.
  9. Emerenciano, K. D. M., Lima, G. L., Peixoto, G. C. X., Silva, M. A., Oliveira, M. G. C., De Paula, V. V., & Silva, A. R. (2013). Recuperação de espermatozoides epididimários de gatos domésticos (Felis catus) utilizando soluções à base de tris ou água de coco em pó. Acta Veterinaria Brasilica, 7(2), 148–153.
  10. Gündoğan, M., Yeni, D., Avdatek, F., & Hazman, Ö. (2021). The effects of CAPE (caffeic acid phenethyl ester) addition on spermatological parameters, oxidative stress and DNA damage after frozen-thawed process in ram semen. Journal of Advances in VetBio Science and Techniques, 6(3), 270-277. https://doi.org/10.31797/vetbio.1008995
  11. Hermansson, U., & Axnér, E. (2007). Epididymal and ejaculated cat spermatozoa are resistant to cold shock but egg yolk promotes sperm longevity during cold storage at 4 °C Theriogenology, 67(7), 1239-1248. https://doi.org/10.1016/j.theriogenology.2007.01.008
  12. Hosnuter, M., Gurel, A., Babuccu, O., Armutcu, F., Kargi, E., & Isikdemir, A. (2004). The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns, 30(2), 121-125. https://doi.org/10.1016/j.burns.2003.09.022
  13. Johnston, S. D., Root-Kustritz, M. V., & Olson, P. N. (2001). Canine and feline theriogenology. (pp. 112-121). Philadelphia.
  14. Klaus, C., Eder, S., Franz, C., & Müller, K. (2016). Successful cryopreservation of domestic cat (felis catus) epididymal sperm after slow equilibration to 15 or 10°C. Reproduction Domestic Animals, 51(2), 195-203. https://doi.org/10.1111/rda.12666
  15. Kunkitti, P., Chatdarong, K., Suwimonteerabutr, J., Nedumpun, T., Johannisson, A., Bergqvist, A. S., Sjunnesson, Y., & Axnér, E. (2017). Osmotic tolerance of feline epididymal spermatozoa. Animal Reproduction Science, 185, 148–153. https://doi.org/10.1016/j.anireprosci.2017.08.014
  16. Kus, I., Colakoglu, N., Pekmez, H., Seckin, D., Ogeturk, M., & Sarsilmaz, M. (2004). Protective effects of caffeic acid phenethyl ester (CAPE) on carbon tetrachloride-induced hepatotoxicity in rats. Acta Histochemica, 106, 289-297. https://doi.org/10.1016/j.acthis.2004.05.002
  17. Luvoni, G. C. (2006). Gamete cryopreservation in the domestic cat. Theriogenology, 66(1), 101-111. https://doi.org/10.1016/j.theriogenology.2006.03.012
  18. Namula, M., Hirata, M., Wittayarat, F., Tanihara, N., Thi, N. T., Hirano, M., & Nii, T. (2018). Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm. Reproduction in Domestic. Animals, 53(6), 1600–1604. https://doi.org/10.1111/rda.13288
  19. Okutan, H., Ozcelik, N., Yilmaz, H.R., & Uz, E. (2005). Effects of caffeic acid phenethyl ester on lipidperoxidation and antioxidant enzymes in diabetic ratheart. Clinical Biochemistry, 38, 191– 196. https://doi.org/10.1016/j.clinbiochem.2004.10.003
  20. Onder, N. T., Gokdemir, T., Kılıc, M. C., Sahin, O., Yıldız, S., Kacar, C., Demir, M. C., & Ozturkler, Y. (2023). Insulin and bull sperm interactions during cryopreservation. Kafkas Universitesi Veteriner Fakultesi Dergisi, 29(4), 401. https://doi.org/10.9775/kvfd.2023.29623
  21. Ozyurt, H., Pekmez, H., Parlaktas, B.S., Kus, I., Ozyurt, B., & Sarsilmaz, M. (2006). Oxidative stress in testicular tissues of rats exposed to cigarette smoke and protective effects of caffeic acid phenethyl ester. Asian Journal of Andrology. 8, 189-193. https://doi.org/10.1111/j.1745-7262.2006.00119.x
  22. Pukazhenthi, B., Spindler, R., Lena, D. W., Bush, M., & Howard, J. G. (2002). Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant. Cryobiology, 44(3), 288-300. https://doi.org/10.1016/S0011-2240(02)00035-4
  23. Rafaei, M. F., & Abdallah, E. A., (2021). Protective effects of caffeic acid phenethyl ester on cadmium-induced testicular injury: A crucial role of antioxidant enzymes in male mice infertility. Heliyon, 7(5), e06965. https://doi.org/10.1016/j.heliyon.2021.e06965
  24. Santos, J. F. P., Gomes, E. T., Siqueira, A. K. M., & Cardoso, R. C. S. (2016). Andrologia e criopreservação de sêmen em cães. Revista Brasileira de Reprodução Animal, 40(4), 167–179.
  25. Schäfer S., & Holzmann, A. (2000). The use of transmigration and Spermac™ stain to evaluate epididymal cat spermatozoa. Animal Reproduction Science, 59, 201-211. https://doi.org/10.1016/s0378-4320(00)00073-7
  26. Soleimanzadeh, A., Talavi, N., Yourdshahi, V. S., & Bucak, M. N. (2020). Caffeic acid improves microscopic sperm parameters and antioxidant status of buffalo (Bubalus bubalis) bull semen following freeze-thawing process. Cryobiology, 95, 29-35. https://doi.org/10.1016/j.cryobiol.2020.06.010
  27. Souza, A. L. P., Lima, G. L., & Silva, A. R. (2014). Alternativas para o aperfeiçoamento dos protocolos de criopreservação de sêmen de animais selvagens. Revista Brasileira de Reprodução Animal, 38(2), 98–102.
  28. Zhang, P., Tang, Y., Li, N.G., Zhu, Y., & Duan, J.A. (2014). Bioactivity and chemical synthesis of caffeic acid phenethyl ester and its derivatives. Molecules, 19, 16458-16476. https://doi.org/ 10.3390/molecules191016458