Dergiler / Aquatic Research / 2020 / Cilt: 3 - Sayı: 3

Determination of serotypic differences of Lactococcus garvieae isolates obtained from rainbow trout farms

Sayfa
135–143
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

According to the results of ELISA test used to determine the serotypical differences of the isolates, two L. garvieae isolates were serotypically different from the other isolates and L. garvieae isolates used in the study formed two different serotypic groups.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:This study aimed to determine the serotypic differences between Lactococcus garvieae strains isolated from rainbow trout obtained from different fish farms in Turkey.
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Temel Bulgular & Veri:It was determined that all 22 L. garvieae isolates used in the study gave a cream colored, bright, rounded smooth S type colony in Triptic Soy Agar (TSA) and all strains were nonmobile in the native examination.
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Sonuç & Katkı:According to the results of ELISA test used to determine the serotypical differences of the isolates, two L. garvieae isolates were serotypically different from the other isolates and L. garvieae isolates used in the study formed two different serotypic groups.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Alan Parametreleri

Özet

This study aimed to determine the serotypic differences between Lactococcus garvieae strains isolated from rainbow trout obtained from different fish farms in Turkey. For this purpose, some phenotypic properties of isolates were determined and then ELISA test was performed to determine serotypic differences. It was determined that all 22 L. garvieae isolates used in the study gave a cream colored, bright, rounded smooth S type colony in Triptic Soy Agar (TSA) and all strains were nonmobile in the native examination. Morphologically, all isolates were found to be Gram positive, nonmobile, α-hemolytic, to have a growth of 0-6.5% NaCl salinity, 21, 37 and 45℃ temperature, and to be oxidase and catalase negative. After examining the biochemical made with API Rapid ID 32 Strep test, it was observed that two L. garvieae isolates were different from the other isolates in terms of sucrose use and one L. garvieae isolate was different from other isolates in terms of maltose profile. According to the results of ELISA test used to determine the serotypical differences of the isolates, two L. garvieae isolates were serotypically different from the other isolates and L. garvieae isolates used in the study formed two different serotypic groups.

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Bu Makaleye Atıf Yap

ÖNALAN, Ş., ARABACI, M., ÇAĞIRGAN, H. (2020). Determination of serotypic differences of Lactococcus garvieae isolates obtained from rainbow trout farms. Aquatic Research, Cilt: 3 - Sayı: 3, 135–143. https://ajindex.com/articles/141942

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Kaynakça (15)

  1. Ağaçfidan, A., Anğ, Ö., Badur, S., Bozkaya, E., Dernetli, Ş., Küçüker, M., Gürler, B., Öner, Y.A., Öngen, B., Töreci, K., Yeğenoğlu, Y. (2002).
  2. Medical Microbiology, Istanbul University Microbiology and Clinical Microbiology Department, Antigen-Antibody Reactions and Indirect Diagnostic Methods, (Ed: Bozkaya, E.) Nobel Medical Bookstore, ISBN: 975-420-230-3.
  3. Altınışık, M. (2004). Immunological Techniques. ADÜTF Biyokimya A.D., 2004. http://www.mustafaaltinisik.org.uk/45-uzm-03.pdf (Access date: 19.04.2013).
  4. Arda, M., Seçer, S., Sarıeyyüpoglu, M. (2002). Fish Diseases. Medisan Publishing Series, 1st Edition, Ankara. ISBN 975-7774-58-8
  5. Austin, B., Austin, D.A. (2007). Bacterial Fish Pathogens Disease of Farmed and Wild Fish, Fourth Edition, Springer Dordrecht Berlin Heidelberg New York, ISBN 978-1-4020- 6068-7.
  6. Austin, B., Austin, D.A. (1999). Bacterial fish pathogens: Disease in Farmed and Wild Fish. 3rd (Revised) Edition.
  7. Praxis Publishing, Chichester, UK. ISBN: 978-1-4020-6069-4
  8. Aydın, N. (1992). Identification of Gram positive, catalase negative cocci isolated from clinical samples. Erciyes University, Faculty of Medicine, Department of Microbiology, (Doctoral dissertation). Health Sciences Institute, 1992.
  9. Balta, F., Dengiz Balta, Z. (2019). The isolation of Lactococcus garvieae from eyes of diseased rainbow trout (Oncorhynchus mykiss) with exopthalmia. Anatolian Environmental and Animal Sciences, 4(1), 27-33. https://doi.org/10.35229/jaes.527258
  10. Baraketi, A., D'Auria, S., Shankar, S., Fraschini, C., Salmieri, S., Menissier, J., Lacroix, M. (2020). Novel spider web trap approach based on chitosan/cellulose nanocrystals/glycerol membrane for the detection of Escherichia coli O157: H7 on food surfaces. International journal of biological macromolecules, 146, 1009-1014. https://doi.org/10.1016/j.ijbiomac.2019.09.225
  11. Barnes, A.C., Ellis, A.E. (2004). Role of Capsule in Serotypic Differences and Complement Fixation by L. garvieae. Fish and Shellfish Immunology, 16, 207–214. https://doi.org/10.1016/S1050-4648(03)00079-2
  12. Beşe, M. (1974). Biochemical Tests and Media Used in Microbiology. Ankara University, Faculty of Veterinary Medicine Publications, 298, p: 96-162,
  13. Ankara.
  14. Beşe, M. (1993). Stains and Dyeing Methods Used in Microbiology. Istanbul University, Faculty of Veterinary Medicine Publications, p: 47-50.
  15. Bortz, B. M. (1984). The immune response in immunized and naturally infected rainbow trout (Salmo gaidneri) Diplostomum spataceum as detected by Enzyme