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Dergiler / International Journal of Secondary Metabolite / 2019 / Volume: 6 Issue: 4

In vitro and in silico Evaluation of Some Natural Molecules as Potent Glutathione Reductase Inhibitors

Tuba Aydin(AGRI IBRAHIM CECEN UNIVERSITY)0000-0002-7653-6480
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Yayın Tarihi
2020-01-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In current study first time, β-sitosterol, stigmasterol, diosgenin and jervine were found to be much more glutathione reductase inhibitors.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Glutathione reductase inhibitors are very popular antimalarial and anticancer agents.
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Temel Bulgular & Veri:According to the results obtained, IC50 values of β-sitosterol, stigmasterol, diosgenin and jervine were found as 1.2580, 5.2116, 0.1916 and 0.7701 µM, respectively.
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Sonuç & Katkı:In current study first time, β-sitosterol, stigmasterol, diosgenin and jervine were found to be much more glutathione reductase inhibitors.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Secondary metabolites · Glutathione reductase · Inhibition

Özet

Glutathione reductase inhibitors are very popular antimalarial and anticancer agents. In this study, in vitro inhibition effects of β-sitosterol, stigmasterol, diosgenin and jervine which containing steroidal structure were determined against glutathione reductase enzyme. β-sitosterol, diosgenin and jervine were isolated from Veratrum album and stigmasterol was isolated from Artemisia dracunculus L. by chromatographic methods. According to the results obtained, IC50 values of β-sitosterol, stigmasterol, diosgenin and jervine were found as 1.2580, 5.2116, 0.1916 and 0.7701 µM, respectively. Among test compounds, diosgenin showed the strongest inhibitory effect against glutathione reductase with Swissdock docking figure. In current study first time, β-sitosterol, stigmasterol, diosgenin and jervine were found to be much more glutathione reductase inhibitors.

Abstract

Glutathione reductase inhibitors are very popular antimalarial and anticancer agents. In this study, in vitro inhibition effects of β -sitosterol, stigmasterol, diosgenin and jervine which containing steroidal structure were determined against glutathione reductase enzyme. β- sitosterol, diosgenin and jervine were isolated from Veratrum album and stigmasterol was isolated from Artemisia dracunculus L. by chromatographic methods. According to the results obtained, IC 50 values of β -sitosterol, stigmasterol, diosgenin and jervine were found as 1.2580, 5.2116, 0.1916 and 0.7701 µM, respectively. Among test compounds, diosgenin showed the strongest inhibitory effect against glutathione reductase with Swissdock docking figure. In current study first time, β -sitosterol, stigmasterol, diosgenin and jervine were found to be much more glutathione reductase inhibitors.

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Bu Makaleye Atıf Yap

Aydin, T. (2020). In vitro and in silico Evaluation of Some Natural Molecules as Potent Glutathione Reductase Inhibitors. International Journal of Secondary Metabolite, Volume: 6 Issue: 4. https://doi.org/10.21448/ijsm.628043

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Kaynakça (26)

  1. . Shahi, Y. Samadi, F.M., Mukherjee, S. (2019). The influence of Tumor Necrosis Factor-alpha gene polymorphism on oxidative stress in patients with oral precancerous lesions and oral cancer, Gene Rep, 17, 1-8. doi: doi.org/10.1016/j.genrep.2019.100525
  2. . Tsai, Y.C., Hong, C.Y., Liu, L.F., Kao C.H. (2005). Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2, J Plant Physiol, 162, 291-299. doi: 10.1016/j.jplph.2004.06.004
  3. . Meister A. Anderson M.E. (1983). Glutathione, Annu Rev Biochem., 52, 711-760. doi: 10.1146/annurev.bi.52.070183.003431
  4. . Schirmer, R.H., Muller, J.G., Krauthsiegel, R.L., (1995). Disulfide-reductase inhibitors as chemotherapeutic - agents - the design of drugs for trypanosomiasis and malaria, Angew Chem Int Edit., 34, 141-154. doi: doi.org/10.1002/anie.199501411
  5. . Bohme, C.C., Arscott, L.D., Becker, K., Schirmer, R.H. (2000). Jr. Williams, C.H. Kinetic characterization of glutathione reductase from the malarial parasite Plasmodium falciparum. Comparison with the human enzyme, J Biol Chem., 275, 37317-37323. doi: 10.1074/jbc.M007695200
  6. . Burkard, L., Scheuermann, A., Simithy, J., Calderon, A.I. (2016). Development of a functional assay to detect inhibitors of Plasmodium falciparum glutathione reductase utilizing liquid chromatography-mass spectrometry, Biomed Chromatogr., 30, 543-547. doi: doi.org/10.1002/bmc.3580
  7. . Talisuna, A.O., Okello, P.E., Erhart, A., Coosemans, M., D'Alessandro, U. (2007). Intensity of malaria transmission and the spread of Plasmodium falciparum-resistant malaria: A review of epidemiologic field evidence, Am J Trop Med Hyg., 77, 170 180. WOS:000252212600027
  8. . Hainley, C.A., Filer, C.N. (2017). Selective D ring side chain tritiation of steroidal natural products, J Radioanal Nucl Ch., 313, 467-472. doi:10.1007/s10967-017-5322-y
  9. . Aydin, T., Cakir, A., Kazaz, C., Bayrak, N., Bayir, Y., Taskesenligil, Y. (2014). Insecticidal metabolites from the rhizomes of Veratrum album against adults of Colorado Potato Beetle, Leptinotarsa decemlineata, Chem Biodivers., 11, 1192-1204. doi:doi.org/10.1002/cbdv.201300407
  10. . Lee, S.T., Panter, K.E., Gaffield, W., Stegelmeier, B.L. (2003). Development of an enzyme-linked immunosorbent assay for the Veratrum plant teratogens: Cyclopamine and jervine, J Agr Food Chem., 51, 582-586. doi: doi.org/10.1021/jf020961s
  11. . Khanfar, M.A., El Sayed, K.A. (2013). The Veratrum alkaloids jervine, veratramine, and their analogues as prostate cancer migration and proliferation inhibitors: biological evaluation and pharmacophore modeling, Med Chem Res, 22, 4775-4786. doi: 10.1007/s00044-013-0495-6
  12. . Yamamoto M. Masui T. Sugiyama K. Yokota M. Nakagomi K. Nakazawa H. (1991). Antiinflammatory active constituents of Aloe arborescens Miller, Agr Biol Chem Tokyo, 55, 1627-1629. doi.org/10.1271/bbb1961.55.1627
  13. . Bouic, P.J.D., Etsebeth, S., Liebenberg, R.W., Albrecht, C.F., Pegel, K., VanJaarsveld, P.P. (1996). Beta-sitosterol and beta-sitosterol glucoside stimulate human peripheral blood lymphocyte proliferation: Implications for their use as an immunomodulatory vitamin combination, Int J Immunopharmaco., 18, 693-700. doi:doi.org/10.1016/S01920561(97)85551-8
  14. . Ju, Y.H., Clausen, L.M., Allred, K.F., Almada, A.L., Helferich, W.G. (2004). beta-Sitosterol, beta-sitosterol glucoside, and a mixture of beta-sitosterol and beta-sitosterol glucoside modulate the growth of estrogen-responsive breast cancer cells in vitro and in ovariectomized athymic mice, J Nutr., 134, 1145 1151. doi:doi.org/10.1093/jn/134.5.1145
  15. . Sethi, G., Shanmugam, M.K., Warrier, S., Merarchi, M., Arfuso, F., Kumar, A.P., Bishayee, A. (2018). Pro-apoptotic and anti-cancer properties of diosgenin: A comprehensive and critical review, Nutrients, 10, 645. doi.org/10.3390/nu10050645
  16. . Aydin, T., Yurtvermez, B., Senturk, M., Kazaz C., Cakir, A. (2019). Inhibitory effects of metabolites isolated from Artemisia dracunculus L. against the human carbonic anhydrase I (hCA I) and II (hCA II), Rec. Nat. Prod., 13, 216-225.doi: doi.org/10.25135/rnp.102.18.07.329
  17. . Kangsamaksin, T., Chaithongyot, S., Wootthichairangsan, C., Hanchaina, R., Tangshewinsirikul, C., Svasti, J. (2017). Lupeol and stigmasterol suppress tumor angiogenesis and inhibit cholangiocarcinoma growth in mice via downregulation of tumor necrosis factor-alpha, PLoS One. 12, e0189628. doi: doi.org/10.1371/journal.pone.0189628
  18. . Beutler, E. (1984). Red cell metabolism. A manual of biochemical methods. Grune and Stratton Inc, Orlando. ISBN: 0808916726 9780808916727
  19. . Balaydin, H.T., Ozil, M., Senturk, M. (2018). Synthesis and glutathione reductase inhibitory properties of 5-methyl-2,4-dihydro-3H-1,2,4-triazol-3-one's aryl Schiff base derivatives, Arch Pharm., 351, 1-8. doi: doi.org/10.1002/ardp.201800086.
  20. . Coban, T.A., Senturk, M., Ciftci, M., Kufrevioglu, O. I. (2007). Effects of some metal ions on human erythrocyte glutathione reductase: an in vitro study, Protein Peptide Lett., 14, 1027-1030. doi: doi.org/10.2174/092986607782541060
  21. . Ray, A., Chatterjee, S., Mukherjee, S., Bhattacharya, S. (2014). Arsenic trioxide induced indirect and direct inhibition of glutathione reductase leads to apoptosis in rat hepatocytes, Biometals., 27, 483-494. doi: doi 10.1007/s10534-014-9722-y
  22. . Seefeldt, T., Zhao, Y., Chen, W., Raza, A.S., Carlson, L., Herman, J., Stoebner, A., Hanson, S., Foll, R., Guan, X.M. (2009). Characterization of a novel dithiocarbamate glutathione reductase inhibitor and its use as a tool to modulate intracellular glutathione, J Biol Chem., 284, 2729-2737. doi: 10.1074/jbc.M802683200
  23. . Kocaoglu, E., Talaz, O., Cavdar, H., Senturk, M., Supuran, C.T., Ekinci, D. (2018). Determination of the inhibitory effects of N-methylpyrrole derivatives on glutathione reductase enzyme, J Enzym Inhib Med Chem., 34, 51-54. doi.org/10.1080/14756366.2018.1520228.
  24. . Senturk, E., Urçar, H., Senturk, M., Yildirim, S., Gul, M., (2016). Bovine liver tissue on glutathione reductase enzyme determination of effects of thiamine, tyrosine, dopamine and adrenaline, Acta Physiol., 218, 58. WOS:000383578300152
  25. . Cakmak, R., Durdagi, S., Ekinci, D., Senturk, M., Topal, G. (2011). Design, synthesis and biological evaluation of novel nitroaromatic compounds as potent glutathione reductase inhibitors, Bioorg Med Chem Lett., 21, 5398-5402. doi: doi.org/10.1016/j.ejmech.2009.03.006
  26. . Chaudhary, S., Chaudhary, P.S., Chikara, S.K., Sharma, M.C., Iriti, M. (2018). Review on Fenugreek (Trigonella foenum-graecum L.) and its important secondary metabolite diosgenin, Not Bot Horti Agrobo., 46, 22-31. doi: doi.org/10.15835/nbha46110996