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Dergiler / International Journal of Secondary Metabolite / 2026 / Volume: 10 Issue: 1

In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts

Elbouny Hamza(Moulay Ismail University)0000-0003-3275-3093
Ouahzizi Brahim(Moulay ismail university)0000-0002-6400-5949
Sellam Khalid(Moulay ismail university)0000-0001-7486-2664
Alem Chakib(Moulay ismail university)0000-0001-6004-7319
Sayfa
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Yayın Tarihi
2023-03-26
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

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Amaç & Hipotez:Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids.
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Temel Bulgular & Veri:Antioxidant (DPPH, FRAP, and TAC methods), antihemolytic (2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) induced hemolysis test), hypolipidemic (3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity inhibition test), and anti-inflammatory (protein denaturation inhibition) effects of extracts were carried out using in vitro methods.
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Sonuç & Katkı:The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Thymus atlanticus · Thymus willdenowii · polyphenols

Özet

Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus (T. atlanticus) and Thymus willdenowii (T. willdenowii) are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant (DPPH, FRAP, and TAC methods), antihemolytic (2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) induced hemolysis test), hypolipidemic (3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity inhibition test), and anti-inflammatory (protein denaturation inhibition) effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test (IC50 = 0.29 mg/mL), while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test (IC50 = 1.61 mg/mL). Moreover, both extracts at a dose of 20 µg/mL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity (T. willdenowii: 51.16 %; T. atlanticus: 62.83 %). In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

Abstract

Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus (T. atlanticus) and Thymus willdenowii (T. willdenowii) are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant (DPPH, FRAP, and TAC methods), antihemolytic (2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) induced hemolysis test), hypolipidemic (3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity inhibition test), and anti-inflammatory (protein denaturation inhibition) effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test (IC50 = 0.29 mg/mL), while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test (IC50 = 1.61 mg/mL). Moreover, both extracts at a dose of 20 µg/mL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity (T. willdenowii: 51.16 %; T. atlanticus: 62.83 %). In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

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Bu Makaleye Atıf Yap

Hamza, E., Brahim, O., Khalid, S., Chakib, A. (2023). In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts. International Journal of Secondary Metabolite, Volume: 10 Issue: 1. https://doi.org/10.21448/ijsm.1110715

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