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Dergiler / International Journal of Secondary Metabolite / 2026 / Volume: 13 Issue: 2

Isolation and characterization of caffeic acid from Cobra Lily (Arisaema propinquum) and evaluation of its biological activities

Saima Hamid(P.G. Department of Environmental Science, University of Kashmir, Srinagar-190006, India)0000-0002-4849-6370
Aarif Nazir(Advanced Research Lab, Department of Zoology, University of Kashmir, Srinagr-190006, Union Territory of Jammu & Kashmir, India.)0009-0005-5318-3538
Muzafar Riyaz(Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu)0000-0001-9372-681X
Mohammad Abass Zargar(Department of Geology, University of Madras, Guindy Campus, Chennai-Tamil Nadu, India.)0009-0009-4353-7477
Arfat Nazir(Department of Geology, University of Madras, Guindy Campus, Chennai-Tamil Nadu, India.)0000-0001-5759-6755
Sayfa
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Yayın Tarihi
2026-06-09
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The findings suggest that the antioxidant and antimicrobial properties of A. propinquum are attributable to its rich phytochemical profile, supporting its potential in therapeutic applications.

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Amaç & Hipotez:This study investigates the antioxidant and antimicrobial potential of Arisaema propinquum, a medicinal herb from the Pir Panjal range of the Kashmir Himalayas.
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Temel Bulgular & Veri:In vitro antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide dismutase (SOD) mimetic, and deoxyribose degradation assays.
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Sonuç & Katkı:The findings suggest that the antioxidant and antimicrobial properties of A. propinquum are attributable to its rich phytochemical profile, supporting its potential in therapeutic applications.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Arisaema propinquum · Medicinal plant · Himalayas

Özet

This study investigates the antioxidant and antimicrobial potential of Arisaema propinquum, a medicinal herb from the Pir Panjal range of the Kashmir Himalayas. Caffeic acid was isolated and characterized from methanolic extracts of its aerial parts. Fourier-transform infrared (FTIR) spectroscopy confirmed the presence of functional groups indicative of polyphenols, flavonoids, terpenoids, esters, and other metabolites. In vitro antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide dismutase (SOD) mimetic, and deoxyribose degradation assays. The deoxyribose assay showed dose-dependent inhibition of 63.2%, 69.7%, and 80.3% at concentrations of 100, 150, and 200 µg/mL, respectively, while the methanolic extract exhibited an IC₅₀ of 49.74 µg/mL in the H₂O₂ scavenging assay. Total phenolic and flavonoid contents were quantified as 40.33 ± 1.9 mg GAE/g DW and 32.77 ± 1.35 mg rutin equivalent/g DW, respectively. In vivo antioxidant activity was evaluated using Saccharomyces cerevisiae, where the extract supported 76.17 ± 0.58% cell survival under oxidative stress. The findings suggest that the antioxidant and antimicrobial properties of A. propinquum are attributable to its rich phytochemical profile, supporting its potential in therapeutic applications.

Abstract

This study investigates the antioxidant and antimicrobial potential of Arisaema propinquum, a medicinal herb from the Pir Panjal range of the Kashmir Himalayas. Caffeic acid was isolated and characterized from methanolic extracts of its aerial parts. Fourier-transform infrared (FTIR) spectroscopy confirmed the presence of functional groups indicative of polyphenols, flavonoids, terpenoids, esters, and other metabolites. In vitro antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, superoxide dismutase (SOD) mimetic, and deoxyribose degradation assays. The deoxyribose assay showed dose-dependent inhibition of 63.2%, 69.7%, and 80.3% at concentrations of 100, 150, and 200 µg/mL, respectively, while the methanolic extract exhibited an IC₅₀ of 49.74 µg/mL in the H₂O₂ scavenging assay. Total phenolic and flavonoid contents were quantified as 40.33 ± 1.9 mg GAE/g DW and 32.77 ± 1.35 mg rutin equivalent/g DW, respectively. In vivo antioxidant activity was evaluated using Saccharomyces cerevisiae, where the extract supported 76.17 ± 0.58% cell survival under oxidative stress. The findings suggest that the antioxidant and antimicrobial properties of A. propinquum are attributable to its rich phytochemical profile, supporting its potential in therapeutic applications.

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Bu Makaleye Atıf Yap

Hamid, S., Nazir, A., Riyaz, M., Zargar, M. A., Nazir, A. (2026). Isolation and characterization of caffeic acid from Cobra Lily (Arisaema propinquum) and evaluation of its biological activities. International Journal of Secondary Metabolite, Volume: 13 Issue: 2. https://doi.org/10.21448/ijsm.1701617

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