KaydedilenlerDerginizi ekleyin

Dergiler / International Journal of 3D Printing Technologies and Digital Industry / 2025 / Cilt: 9 Sayı: 3

NEURONAL TISSUE MODELLING WITH 3D BIOPRINTING

Burçak Yavuz(Altınbaş Üniversitesi)0000-0002-3555-4850
Duygu Tunçman Kayaokay(İSTANBUL ÜNİVERSİTESİ-CERRAHPAŞA, SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0002-0929-0441
Sayfa
588–598
Yayın Tarihi
2025-12-28
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Ultimately, the findings pave the way for creating more precise and effective biofabrication strategies, emphasizing the need for tailored bioink formulations that support specific biological outcomes in various applications.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:In this study, SH-SY5Y neuroblastoma cells were bioprinted using a GelMA/HAMA bioink, and the formation of spheroids was observed to initiate as early as day 3.
📊
Temel Bulgular & Veri:This observation is significant as it indicates that the specific composition of the bioink can have a profound impact on cellular behavior and the spheroid formation process.
💡
Sonuç & Katkı:Ultimately, the findings pave the way for creating more precise and effective biofabrication strategies, emphasizing the need for tailored bioink formulations that support specific biological outcomes in various applications.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Bioprinting · Spheroid · Neuronal Tissue

Özet

In this study, SH-SY5Y neuroblastoma cells were bioprinted using a GelMA/HAMA bioink, and the formation of spheroids was observed to initiate as early as day 3. This observation is significant as it indicates that the specific composition of the bioink can have a profound impact on cellular behavior and the spheroid formation process. Comparatively, in earlier studies utilizing an alginate/gelatin-based bioink, spheroid formation only became noticeable around day 5, suggesting that the GelMA/HAMA bioink provides a more conducive environment for earlier cellular aggregation and organization. The results of this study highlight the critical role of bioink formulation in influencing the biological responses of cells within a three-dimensional (3D) microenvironment. The quicker spheroid formation observed with the GelMA/HAMA bioink may be attributed to its biochemical properties, such as enhanced cell adhesion, improved mechanical strength, and the ability to mimic the extracellular matrix. These properties provide an optimized platform for cell-cell and cell-matrix interactions, promoting accelerated tissue-like structures. This research underscores the importance of systematically investigating the effects of bioink compositions on cellular biology. Understanding the interplay between bioink components and cellular behavior is essential for improving the design and application of bioprinting technologies. Such insights are invaluable for advancing tissue engineering, regenerative medicine, and the development of disease models. Ultimately, the findings pave the way for creating more precise and effective biofabrication strategies, emphasizing the need for tailored bioink formulations that support specific biological outcomes in various applications.

Abstract

In this study, SH-SY5Y neuroblastoma cells were bioprinted using a GelMA/HAMA bioink, and the formation of spheroids was observed to initiate as early as day 3. This observation is significant as it indicates that the specific composition of the bioink can have a profound impact on cellular behavior and the spheroid formation process. Comparatively, in earlier studies utilizing an alginate/gelatin-based bioink, spheroid formation only became noticeable around day 5, suggesting that the GelMA/HAMA bioink provides a more conducive environment for earlier cellular aggregation and organization. The results of this study highlight the critical role of bioink formulation in influencing the biological responses of cells within a three-dimensional (3D) microenvironment. The quicker spheroid formation observed with the GelMA/HAMA bioink may be attributed to its biochemical properties, such as enhanced cell adhesion, improved mechanical strength, and the ability to mimic the extracellular matrix. These properties provide an optimized platform for cell-cell and cell-matrix interactions, promoting accelerated tissue-like structures. This research underscores the importance of systematically investigating the effects of bioink compositions on cellular biology. Understanding the interplay between bioink components and cellular behavior is essential for improving the design and application of bioprinting technologies. Such insights are invaluable for advancing tissue engineering, regenerative medicine, and the development of disease models. Ultimately, the findings pave the way for creating more precise and effective biofabrication strategies, emphasizing the need for tailored bioink formulations that support specific biological outcomes in various applications.

🔖

Bu Makaleye Atıf Yap

Yavuz, B., Kayaokay, D. T. (2025). NEURONAL TISSUE MODELLING WITH 3D BIOPRINTING. International Journal of 3D Printing Technologies and Digital Industry, Cilt: 9 Sayı: 3, 588–598. https://doi.org/10.46519/ij3dptdi.1631511

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (62)

  1. Radenkovic, D., Solouk, A., and Seifalian, A., “Personalized development of human organs using 3D printing technology”, Medical Hypotheses, Vol. 87, Pages 30–33, 2016.
  2. Tack, P., Victor, J., Gemmel, P., and Annemans, L., “3D-printing techniques in a medical setting: a systematic literature review”, Biomedical Engineering Online, Vol. 15, Pages 1-21, 2016.
  3. Jian, H., Wang, M., Wang, S., Wang, A., and Bai, S., “3D bioprinting for cell culture and tissue fabrication”, Bio-Design and Manufacturing, Vol. 1, Pages 45-61, 2018.
  4. Jang, J., Park, H.J., Kim, S.W., Kim, H., Park, J.Y., Na, S.J., Kim, H.J., Park, M.N., Choi, S.H., Park, S.H., Kim, S.W., Kwon, S., Kim, P., and Cho, D., “3D printed complex tissue construct using stem cell-laden decellularized extracellular matrix bioinks for cardiac repair”, Biomaterials, Vol. 112, Pages 264-274, 2017.
  5. Ozbolat, I.T., “Bioprinting scale-up tissue and organ constructs for transplantation”, Trends in Biotechnology, Vol. 33, Pages 395–400, 2015.
  6. Sensharmaa, P., Rahul, G.M. Jayantc, D. and Amit, K., “Biomaterials and cells for neural tissue engineering: Current choices”, Materials Science and Engineering: C, Vol. 7, Pages 1302-1315, 2017.
  7. Decante, G., Costa, J. B., Silva-Correia, J., Collins, M. N., Reis, R. L., and Oliveira, J. M., “Engineering bioinks for 3D bioprinting”, Biofabrication, Vol. 13, Issue 3, Pages 1-20, 2021.
  8. Gopinathan, J., and Noh, I., “Recent trends in bioinks for 3D printing”, Biomaterials Research, Vol. 22, Pages 1-15, 2018.
  9. Choudhury, D., Anand, S., and Naing, M. W., “The arrival of commercial bioprinters–Towards 3D bioprinting revolution!”, International Journal of Bioprinting, Vol. 4, Issue 2, Pages 1-20, 2018.
  10. O'brien, F. J., “Biomaterials & scaffolds for tissue engineering”, Materials Today, Vol. 14, Issue, 3, Pages 88-95, 2011.
  11. Chimene, D., Kaunas, R., and Gaharwar, A. K., “Hydrogel bioink reinforcement for additive manufacturing: a focused review of emerging strategies”, Advanced Materials, Vol. 32, Issue 1, Pages 1-22, 2020.
  12. Cesarz, Z., and Tamama, K., “Spheroid culture of mesenchymal stem cells”, Stem Cells International, Vol. 2016, Issue 1, Pages 1-11, 2016.
  13. Iwami, K., Noda, T., Ishida, K., Morishima, K., Nakamura, M., and Umeda, N., “Bio rapid prototyping by extruding/ aspirating/ refilling thermoreversible hydrogel”, Biofabrication, Vol. 2, Issue 1, Pages 1-11, 2010.
  14. Xu, T., Zhao, W., Zhu, J. M., Albanna, M. Z., Yoo, J. J., and Atala, A., “Complex heterogeneous tissue constructs containing multiple cell types prepared by inkjet printing technology”, Biomaterials. Vol. 34, Issue 1, Pages 130-139, 2013.
  15. Karzyński, K., Kosowska, K., Ambrożkiewicz, F., Berman, A., Cichoń, J., Klak, M., Serwanska-Swiete, M., and Wszola, M., “Use of 3D bioprinting in biomedical engineering for clinical application”, Medical Studies/Studia Medyczne. Vol. 34, Issue 1, Pages 93-97, 2018.
  16. Derakhshanfar, S., Mbeleck, R., Xu, K., Zhang, X., Zhong, W., and Xing, M., “3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances”, Bioactive materials, Vol. 3, Issue 2, Pages 144-156, 2018.
  17. Marga, F., Jakab, K., Khatiwala, C., Shepherd, B., Dorfman, S., Hubbard, B., and Forgacs, G., “Toward engineering functional organ modules by additive manufacturing”, Biofabrication, Vol. 4, Issue 2, Pages 1-13, 2012.
  18. Kolesky, D.B., Homan, K. A., Skylar-Scott, M. A., and Lewis, J. A. “Three-dimensional bioprinting of thick vascularized tissues”, Proceedings of The National Academy of Sciences. Vol. 113, Issue 12, Pages 3179-3184, 2016.
  19. Chang, R., Nam, J. A. E., and Sun, W. E. I., “Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication–based direct cell writing”, Tissue Engineering Part A, Vol. 14, Issue 1, Pages 41-48, 2008.
  20. Kim, M. H., Lee, Y. W., Jung, W. K., Oh, J., and Nam, S. Y., “Enhanced rheological behaviors of alginate hydrogels with carrageenan for extrusion-based bioprinting”, Journal of the Mechanical Behavior of Biomedical Materials, Vol. 98, Pages 187-194, 2019.
  21. Ramesh, S., Harrysson, O. L., Rao, P. K., Tamayol, A., Cormier, D. R., Zhang, Y., and Rivero, I.V., “Extrusion bioprinting: Recent progress, challenges, and future opportunities”, Bioprinting, Vol. 21, Pages 1-19, 2021. 22. Duan, B., Hockaday, L. A., Kang, K. H., and Butcher, J. T., “3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels”, Journal of Biomedical Materials Research Part A, Vol. 101, Issue 5, Pages 1255-1264, 2013.
  22. Pati, F., Ha, D. H., Jang, J., Han, H. H., Rhie, J. W., and Chao, DW., “Biomimetic 3D tissue printing for soft tissue regeneration”, Biomaterials, Vol. 62, Pages 164-175, 2015.
  23. Pati, F., Jang, J., Ha, D. H., Won Kim, S., Rhie, J. W., Shim, J. H., and Cho, D. W., “Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink”, Nature Communications, Vol. 5, Issue 1, Pages 1-11, 2014.
  24. Shim, J. H., Lee, J. S., Kim, J. Y., and Cho, D. W., “Bioprinting of a mechanically enhanced three-dimensional dual cell-laden construct for osteochondral tissue engineering using a multi-head tissue/organ building system”, Journal of Micromechanics and Microengineering, Vol. 22, Issue 8, Pages 1-12, 2012.
  25. Lee, W., Debasitis, J. C., Lee, V. K., Lee, J. H., Fischer, K., Edminster, K., Park, J.K., and Yoo, S.S., “Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication”, Biomaterials, Vol. 30, Issue 8, Pages 1587-1595, 2009.
  26. Arrigoni, C., Petta, D., Bersini, S., Mironov, V., Candrian, C., and Moretti, M., “Engineering complex muscle-tissue interfaces through microfabrication”, Biofabrication, Vol. 11, Issue 3, Pages 1-19, 2019.
  27. Lee, V. K., Lanzi, A. M., Ngo, H., Yoo, S. S., Vincent, P. A., and Dai, G., “Generation of multi-scale vascular network system within 3D hydrogel using 3D bio-printing technology”, Cellular and Molecular Bioengineering, Vol. 7, Issue 3, Pages 460-472, 2004.
  28. Gudapati, H., Dey, M., and Ozbolat, I. A., “Comprehensive review on droplet-based bioprinting: Past, present and future”, Biomaterials, Vol. 102, Pages 20-42, 2016.
  29. Cui, M., Zhan, T., Yang, J., Dang, H., Yang, G., Han, H., Liu, L., and Xu, Y., “Droplet Generation, Vitrification, and Warming for Cell Cryopreservation: A Review”, ACS Biomaterials Science & Engineering, Vol. 9, Issue 3, Pages 1151-1163, 2023.
  30. Hansen, C. J., Saksena, R., Kolesky, D. B., Vericella, J. J., Kranz, S. J., Muldowney, G. P., Christensen, K.T., and Lewis, J.A., “High‐throughput printing via microvascular multinozzle arrays”, Advanced Materials. Vol. 25, Issue 1, Pages 96-102, 2013.
  31. Xu, T., Kincaid, H., Atala, A., and Yoo, J. J. “High-throughput production of single-cell microparticles using an inkjet printing technology”, Journal of Manufacturing Science and Engineering, Vol. 130, Issue 2, Pages 1-5, 2008.
  32. Cui, X., and Boland, T., “Human microvasculature fabrication using thermal inkjet printing technology”, Biomaterials, Vol. 30, Issue 31, Pages 6221-6227, 2009.
  33. Cui, X., Breitenkamp, K., Finn, M. G., Lotz, M., and D'Lima, D. D., “Direct human cartilage repair using three-dimensional bioprinting technology”, Tissue Engineering Part A. Vol. 18, Issue 11-12, 1304-1312, 2012.
  34. Skardal, A., Mack, D., Kapetanovic, E., Atala, A., Jackson, J. D., Yoo, J., and Soker, S., “Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds”, Stem cells translational medicine, Vol. 1, Issue 11, Pages 792-802, 2012.
  35. Odde, D. J., and Renn, M. J. “Laser-guided direct writing for applications in biotechnology”, Trends in biotechnology, Vol. 17, Issue 10, Pages 385-389, 1999.
  36. Guillemot, F., Souquet, A., Catros, S., and Guillotin, B., “Laser-assisted cell printing: principle, physical parameters versus cell fate and perspectives in tissue engineering”, Nanomedicine. Vol. 5, Issue 3, Pages 507-515, 2010.
  37. Nguyen, A. K., and Narayan, R. J., “Liquid-phase laser-induced forward transfer for complex organic inks and tissue engineering”, Annals of biomedical engineering, Vol. 45, Issue 1, Pages 84-99, 2017.
  38. Catros, S., Fricain, J. C., Guillotin, B., Pippenger, B., Bareille, R., Remy, M., Lebraud, E., Desbat, B., Amédée J., and Guillemot, F., “Laser-assisted bioprinting for creating on-demand patterns of human osteoprogenitor cells and nano-hydroxyapatite”, Biofabrication, Vol. 3, Issue 2, Pages 1-12, 2011.
  39. 40 Ozbolat, I. T., 6-Laser-Based Bioprinting with Minor Contributions by Hemanth Gudapati, The Pennsylvania State University. 3D Bioprint; Ozbolat, IT, Ed.; Academic Press: Oxford, UK, 165-197, 2017.
  40. Zennifer, A., Subramanian, A., and Sethuraman, S. “Design considerations of bioinks for laser bioprinting technique towards tissue regenerative applications”, Bioprinting, Vol. 27, Pages 1-17, 2022.
  41. Schiele, N. R., Corr, D. T., Huang, Y., Raof, N. A., Xie, Y., and Chrisey, D. B., “Laser-based direct-write techniques for cell printing”, Biofabrication, Vol. 2, Issue 3, Pages 1-14, 2010.
  42. Michael, S., Sorg, H., Peck, C. T., Koch, L., Deiwick, A., Chichkov, B., Vogt, P.M., and Reimers, K., “Tissue-engineered skin substitutes created by laser-assisted bioprinting form skin-like structures in the dorsal skin fold chamber in mice”, PloS one, Vol. 8, Issue 3, Pages 1-12, 2013.
  43. Keriquel, V., Oliveira, H., Rémy, M., Ziane, S., Delmond, S., and Rousseau, B., “In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications”, Scientific Reports, Vol. 7, Issue 1, Pages 1-10, 2017.
  44. Morris, V. B., Nimbalkar, S., Younesi, M., McClellan, P., and Akkus, O., “Mechanical properties, cytocompatibility and manufacturability of chitosan: PEGDA hybrid-gel scaffolds by stereolithography”, Annals of biomedical engineering, Vol. 45, Issue 1, Pages 286-296, 2017.
  45. Bártolo, P. J., “Stereolithographic processes. In Stereolithography: Materials, Processes and Applications”, MA: Springer, Boston, Pages 1-36, 2009.
  46. Agarwal, S., Saha, S., Balla, V. K., Pal, A., Barui, A., and Bodhak, S., “Current developments in 3D bioprinting for tissue and organ regeneration–a review”, Frontiers in Mechanical Engineering, Vol. 6, Pages 1-22, 2020.
  47. Childress, K. K., Alim, M. D., Hernandez, J. J., Stansbury, J. W., and Bowman, C. N., “Additive manufacture of lightly crosslinked semicrystalline thiol–enes for enhanced mechanical performance”, Polymer Chemistry, Vol. 11, Issue 1, Pages 39-46, 2020.
  48. Ravnic, D. J., Leberfinger, A. N., Koduru, S. V., Hospodiuk, M., Moncal, K. K., Datta, P., Dey, M., Rizk, E., and Ozbolat, I.T., “Transplantation of bioprinted tissues and organs: Technical and clinical challenges and future perspectives”, Annals of Surgery, Vol. 266, Issue 1, Pages 48-58, 2017.
  49. Gao, G., Schilling, A. F., Hubbell, K., Yonezawa, T., Truong, D., Hong, Y., Dai, G., and Cui, X., “Improved properties of bone and cartilage tissue from 3D inkjet-bioprinted human mesenchymal stem cells by simultaneous deposition and photocrosslinking in PEG-GelMA”, Biotechnology Letters, Vol. 37, Issue 11, Pages 2349-2355, 2015.
  50. Müller, M., Öztürk, E., Arlov, Ø., Gatenholm, P., and Zenobi-Wong, M., “Alginate sulfate–nanocellulose bioinks for cartilage bioprinting applications”, Annals of Biomedical Engineering, Vol. 45, Issue 1, Pages 210-223, 2017.
  51. Lam, T., Dehne, T., Krüger, J. P., Hondke, S., Endres, M., Thomas, A., Lauster, R., Sittinger, M., and Kloke, L., “Photopolymerizable gelatin and hyaluronic acid for stereolithographic 3D bioprinting of tissue‐engineered cartilage”, Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 107, Issue 8, Pages 2649-2657, 2019.
  52. Gao, G., Yonezawa, T., Hubbell, K., Dai, G., and Cui, X., “Inkjet‐bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging”, Biotechnology Journal, Vol. 10, Issue 10, Pages 1568-1577, 2015.
  53. Bendtsen, S. T., Quinnell, S. P., and Wei, M., “Development of a novel alginate‐polyvinyl alcohol‐hydroxyapatite hydrogel for 3D bioprinting bone tissue engineered scaffolds”, Journal of Biomedical Materials Research Part A, Vol. 105, Issue 5, Pages 1457-1468, 2017.
  54. Jeon, H., Kang, K., Park, S. A., Kim, W. D., Paik, S. S., Lee, S. H., Jeong, J., and Choi, D., “Generation of multilayered 3D structures of HepG2 cells using a bio-printing technique”, Gut and Liver, Vol. 11, Issue 1, Pages 121-128, 2017.
  55. Wang, Z., Lee, S. J., Cheng, H. J., Yoo, J. J., and Atala, A., “3D bioprinted functional and contractile cardiac tissue constructs”, Acta Biomaterialia, Vol. 70, Pages 48-56, 2018.
  56. Tse, C., Whiteley, R., Yu, T., Stringer, J., MacNeil, S., Haycock, J. W., and Smith, P. J., “Inkjet printing Schwann cells and neuronal analogue NG108-15 cells”, Biofabrication, Vol. 8, Issue 1, Pages 1-9, 2016.
  57. Bertassoni, L. E., Cecconi, M., Manoharan, V., Nikkhah, M., Hjortnaes, J., Cristino, A. L., Barabaschi, G., Demarchi, D., Dokmeci, M.R., Yang, Y., and Khademhosseini, A., “Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs”, Lab on a Chip, Vol. 14, Issue 13, Pages 2202-2211, 2014.
  58. Fan, R., Piou, M., Darling, E., Cormier, D., Sun, J., and Wan, J., “Bio-printing cell-laden Matrigel–agarose constructs”, Journal of Biomaterials Applications, Vol. 31, Issue 5, Pages 684-692, 2016.
  59. Xu, C., Chai, W., Huang, Y., and Markwald, R. R. “Scaffold‐free inkjet printing of three‐dimensional zigzag cellular tubes”, Biotechnology and Bioengineering, Vol. 109, Issue 12, Pages 3152-3160, 2012.
  60. Benam, K. H., Dauth, S., Hassell, B., Herland, A., Jain, A., Jang, K. J., and Ingber, D. E., “Engineered in vitro disease models”, Annual Review of Pathology: Mechanisms of Disease, Vol. 10, Issue 1, Pages 195-262, 2015.
  61. Yavuz, B., Yildiz, A. P. Z., Abamor, E. S., Darici, H., and Allahverdiyev, A. M., “Neuroprotective effects of Wharton jelly stem cell-derived exosomes developed as nano-drug delivery system in 6-OHDA-induced neurotoxicity in 2D and 3D neuronal cell line”, Regenerative Engineering and Translational Medicine, Vol. 10, Issue 2, Pages 243-252, 2024.
  62. Wang, G., An, Y., Zhang, X., Ding, P., Bi, H., and Zhao, Z., “Chondrocyte spheroids laden in GelMA/HAMA hybrid hydrogel for tissue-engineered cartilage with enhanced proliferation, better phenotype maintenance, and natural morphological structure”, Gels, Vol. 7, Issue 4, Pages 1-16, 2021.