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Dergiler / International Advanced Researches and Engineering Journal / 2026 / Volume: 5 Issue: 1

Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer

Özlem Kaplan(Istanbul University, Department of Molecular Biology and Genetics, Istanbul, Turkey)0000-0002-3052-4556
Rizvan İmamoğlu(Bartın University, Department of Biotechnology, Bartın, 74110, Turkey)0000-0002-6306-4760
İskender Şahingöz(Tokat Gaziosmanpaşa University, Department of Genetic and Bioengineering, Tokat, 60250, Turkey)0000-0003-4127-4772
İsa Gökçe(Tokat Gaziosmanpaşa University, Department of Genetic and Bioengineering, Tokat, 60250, Turkey)0000-0002-5023-9947
Sayfa
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Yayın Tarihi
2021-04-15
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings.
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Temel Bulgular & Veri:In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR.
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Sonuç & Katkı:It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Polymerase Chain Reaction · Single Strand Binding Protein · Thermus aquaticus

Abstract

Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings. In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR. Thermostable SSB protein eliminates secondary structure or dimer formation and significantly increase the effectiveness of amplification of DNA fragments. In this study, it was ensured that the SSB gene of thermophilic bacteria Thermus aquaticus (T. aquaticus) was cloned into the pET28b vector and expressed in E. coli BL21 (DE3) PLysE cells. Then, the purification of the SSB protein produced in E. coli BL21 (DE3) PLysE cells was performed. 20 mg SSB protein was obtained from 1L bacterial culture, and its purity was more than 90%. It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.

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Bu Makaleye Atıf Yap

Kaplan, Ö., İmamoğlu, R., Şahingöz, İ., Gökçe, İ. (2021). Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer. International Advanced Researches and Engineering Journal, Volume: 5 Issue: 1. https://doi.org/10.35860/iarej.766741

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