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Dergiler / Eurasian Journal of Forest Science / 2026 / Cilt: 8 Sayı: 2

DNA barcoding, phytochemical screening and antimicrobial activity of Rhododendron arboreum, a high altitudinal medicinal plant from Nepal

Deepak Sharma(Kathmandu University)
Tribikram Bhandari(Kathmandu University)
Anaviggha Pradhan(Kathmandu University)
Navin Ghimire(Kathmandu University)
Sagar Basnet(Kathmandu University)
Saroj Pandey(Kathmandu University)
Janardan Lamichhane(Kathmandu University)
Sayfa
140–151
Yayın Tarihi
2020-06-15
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The rbcL and MatK gene loci show the 99.98% similarly for the R.arboreum in NCBI database and were submitted to the BOLD database for the final authentication and get success for the first time to get published DNA barcode of Nepalese plants.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:DNA barcoding is been proposed as a powerful taxonomic tool for species identification genetically.
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Temel Bulgular & Veri:Rhododendron of Nepal shows genetic diversity due to which 31 different species were reported till date, found in Nepal based on morphological character.
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Sonuç & Katkı:The rbcL and MatK gene loci show the 99.98% similarly for the R.arboreum in NCBI database and were submitted to the BOLD database for the final authentication and get success for the first time to get published DNA barcode of Nepalese plants.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:DNA Barcode · rbcL+matK · Antimicrobial

Abstract

DNA barcoding is been proposed as a powerful taxonomic tool for species identification genetically. The Consortium for the Barcode of Life (CBOL) Plant Working Group has recommended the combination of rbcL & matK as the core plant barcode. Rhododendron of Nepal shows genetic diversity due to which 31 different species were reported till date, found in Nepal based on morphological character. We collected different samples from the central and western parts of Nepal (Province 3 & 5). For the phytochemical study, cold extraction was performed using methanol as solvents. The crude extracts were tested for the presence of phytochemicals such as alkaloids, saponins, glycosides, tannins, flavonoids, and coumarins. The same extracts were used for antimicrobial assay five different pathogenic micro-organisms. MIC was done for calculating the minimum effective concentration of the extract. Then TLC was done to separate the compound. The methanol extract was analyzed for core secondary metabolites by HPLC-QTOF and found more than 20 medicinal important compounds. DPPH assay was also done with crude extracts for identifying antioxidant potency of the extracts. Among samples compared, Gulmi and Palpa showed the best result for antioxidant assay with minimum IC50. Similarly, phenolic content was also checked and samples from Palpa showed high phenolic content. The rbcL and MatK gene loci show the 99.98% similarly for the R.arboreum in NCBI database and were submitted to the BOLD database for the final authentication and get success for the first time to get published DNA barcode of Nepalese plants.

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Bu Makaleye Atıf Yap

Sharma, D., Bhandari, T., Pradhan, A., Ghimire, N., Basnet, S., Pandey, S., Lamichhane, J. (2020). DNA barcoding, phytochemical screening and antimicrobial activity of Rhododendron arboreum, a high altitudinal medicinal plant from Nepal. Eurasian Journal of Forest Science, Cilt: 8 Sayı: 2, 140–151. https://doi.org/10.31195/ejejfs.696768

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Kaynakça (42)

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  3. Kress, W.J., Wurdack, K.J., Zimmer, E.A., Weigt, L.A., and Janzen, D.H. (2005). Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci U S A 102, 8369-8374
  4. Hebert, P.D.N., Cywinska, A., Ball, S.L., and deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proc Biol Sci 270, 313-321
  5. Li, Y., Tong, Y., and Xing, F. (2016). DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China. Frontiers in Plant Science 7
  6. Wong, K.-L., But, P., and Shaw, P.-C. (2013). Evaluation of seven DNA barcodes for differentiating closely related medicinal Gentiana species and their adulterants. Chinese medicine 8, 16
  7. Newmaster, S.G., Fazekas, A.J., Steeves, R.A.D., and Janovec, J. (2008). Testing candidate plant barcode regions in the Myristicaceae. Mol Ecol Resour 8, 480-490
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  23. COX PA, C.K. (1997). Encyclopedia of Rhododendron species. In Perth: Glendoick Publishing;
  24. Kress, W.J., Wurdack, K.J., Zimmer, E.A., Weigt, L.A., and Janzen, D.H. (2005). Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci U S A 102, 8369-8374
  25. Hebert, P.D.N., Cywinska, A., Ball, S.L., and deWaard, J.R. (2003). Biological identifications through DNA barcodes. Proc Biol Sci 270, 313-321
  26. Li, Y., Tong, Y., and Xing, F. (2016). DNA Barcoding Evaluation and Its Taxonomic Implications in the Recently Evolved Genus Oberonia Lindl. (Orchidaceae) in China. Frontiers in Plant Science 7
  27. Wong, K.-L., But, P., and Shaw, P.-C. (2013). Evaluation of seven DNA barcodes for differentiating closely related medicinal Gentiana species and their adulterants. Chinese medicine 8, 16
  28. Newmaster, S.G., Fazekas, A.J., Steeves, R.A.D., and Janovec, J. (2008). Testing candidate plant barcode regions in the Myristicaceae. Mol Ecol Resour 8, 480-490
  29. Garg, N. (2017). Chapter 8 - Technology for the Production of Agricultural Wines. In Science and Technology of Fruit Wine Production, M.R. Kosseva, V.K. Joshi and P.S. Panesar, eds. (San Diego: Academic Press), pp. 463-486
  30. Scott (2010). NEPAL'S MAGNIFICENT RHODODENDRON. In ECS Nepal
  31. Mainra, A., Badola, H., and Mohanty, B. (2010). RHODODENDRONS: CONSERVATION AND SUSTAINABLE USE International Conference RHODODENDRONS: CONSERVATION AND SUSTAINABLE USE. 11. pokhrel (1999)
  32. Paul, A., Khan, M., and Arunachalam, A. (2005). Biodiversity and conservation of rhododendrons in Arunachal Pradesh in the Indo-Burma biodiversity hotspot. Current Science 89
  33. Shrivastav, P. (2012). Journal of Applied Pharmaceutical Science
  34. Andriani, Y., Ramli, N.M., Syamsumir, D.F., Kassim, M.N.I., Jaafar, J., Aziz, N.A., Marlina, L., Musa, N.S., and Mohamad, H. (2019). Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits. Arabian Journal of Chemistry 12, 3555-3564
  35. Deepak, S., Meena maiy, S., and Hitesh, B. (2019). The Effectiveness of Anti-Cancer Compounds Taxol Derived from Natural Products from Nepal. Acta Scientific Pharmaceutical Sciences 3, 116-123
  36. Venugopal, C., McFarlane, N.M., Nolte, S., Manoranjan, B., and Singh, S.K. (2012). Processing of primary brain tumor tissue for stem cell assays and flow sorting. J Vis Exp, 4111
  37. Sharma, D., Shrestha, T., Rapal, N., Poudel, S.C., Shrestha, S., Sharma, S., Poudel, K.P., and Lamichane, J. (2016). Screening and bioactivity measurement of high altitude medicinal plants of Nepal. Vegetos- An International Journal of Plant Research 29
  38. Deepak, S., Lamichhane J (2019)
  39. Kress, W.J. (2017). Plant DNA barcodes: Applications today and in the future. Journal of Systematics and Evolution 55, 291-307
  40. Hollingsworth, P.M., Forrest, L.L., Spouge, J.L., Hajibabaei, M., Ratnasingham, S., van der Bank, M., Chase, M.W., Cowan, R.S., Erickson, D.L., Fazekas, A.J., et al. (2009). A DNA barcode for land plants. Proceedings of the National Academy of Sciences 106, 12794-12797
  41. Stecher, G., Tamura, K., and Kumar, S. (2020). Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Mol Biol Evol
  42. Bold (2013).