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Dergiler / Avrupa Bilim ve Teknoloji Dergisi / 2020 / Sayı: 20

Farmasötik Dozaj, İnsan Serumu ve İdrarındaki Siprofloksasinin Moleküler Baskılı Polipirol Modifiye Elektrot Kullanılarak Voltametrik Tayini

Determination Of Ciprofloxacin In Pharmaceutical Dosage, Human Serum and Urine, Using Molecularly Imprinted Polymer Modified Electrode By Voltammetry

Berrin Gürler Akyüz(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ)0000-0002-8711-2384
Sabriye Perçin Özkorucuklu(İstanbul Üniversitesi, Moleküler Biyoloji ve Genetik Bölümü)0000-0001-9778-2034
Esengül Kır(Süleyman Demirel Üniversitesi, Kimya Bölümü)0000-0002-6641-3444
Gizem Yıldırım Baştemur(İstanbul Üniversitesi, Moleküler Biyoloji ve Genetik Bölümü)0000-0002-4634-4525
Sayfa
859–865
Yayın Tarihi
2020-12-31
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Önerilen yöntem ilaçta, insan kanı ve idrarındaki siprofloksasinin tayini için başarıyla uygulanmıştır.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Siprofloksasin tayini için moleküler baskılanmış polimer bazlı çalışma elektrodu hazırlanmıştır.
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Temel Bulgular & Veri:Siprofloksasinin elektrokimyasal davranışı % 10-60 (v/v) asetonitril -su ikili karışımında ve pH 3-7 aralığında hazırlanan Britton-Robinson tamponu kullanılarak diferansiyel puls voltametrisi yöntemiyle incelenmiştir.
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Sonuç & Katkı:Önerilen yöntem ilaçta, insan kanı ve idrarındaki siprofloksasinin tayini için başarıyla uygulanmıştır.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Siprofloksasin · Polipirol · Biyolojik numune

Özet

Siprofloksasin tayini için moleküler baskılanmış polimer bazlı çalışma elektrodu hazırlanmıştır. Siprofloksasinin elektrokimyasal davranışı % 10-60 (v/v) asetonitril -su ikili karışımında ve pH 3-7 aralığında hazırlanan Britton-Robinson tamponu kullanılarak diferansiyel puls voltametrisi yöntemiyle incelenmiştir. Voltametrik analizler moleküler baskılanmış (MIP) ve baskılanmamış (NIP) modifiye elektrotlar ile gerçekleştirilmiştir. Elektropolimerizasyon döngü sayısı, çözelti pH'sı ve asetonitril-su oranının her iki tip elektrodun performansı üzerindeki etkisi araştırılmış ve optimize edilmiştir. Dedeksiyon limiti 3.34×10-5 M (S/N = 3) olarak bulunmuştur. Önerilen yöntem ilaçta, insan kanı ve idrarındaki siprofloksasinin tayini için başarıyla uygulanmıştır.

Abstract

Molecularly imprinted polymer based working electrode was prepared for the determination of ciprofloxacin (CF). Electrochemical behavior of CF was investigated by differential pulse voltammetric (DPV) method in the range of 10-60% (v/v) acetonitrile (MeCN)-H2O binary mixture at the pH between 3-7 prepared Britton-Robinson (BR) buffers. Voltammetric analyzes were performed with molecularly imprinted (MIP) and non-imprinted (NIP) modified electrodes. The influence of the electropolymerization cycles, solution pH, and MeCN-H2O ratio, on the performance of both electrodes were evaluated and optimized. Detection limit was obtained as 3.34×10-5 M (S/N=3). Recommended method was successfully employed for determination of CF in pharmaceutical, human serum and urine.

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Bu Makaleye Atıf Yap

Akyüz, B. G., Özkorucuklu, S. P., Kır, E., Baştemur, G. Y. (2020). Farmasötik Dozaj, İnsan Serumu ve İdrarındaki Siprofloksasinin Moleküler Baskılı Polipirol Modifiye Elektrot Kullanılarak Voltametrik Tayini. Avrupa Bilim ve Teknoloji Dergisi, Sayı: 20, 859–865. https://doi.org/10.31590/ejosat.796654

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