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Dergiler / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2026 / Volume: 73 Issue: 2

Impact of lysozyme on ruminal fermentation and microbial population in Rusitec fermenters

Saad Ahmed Adam Musa(ANKARA ÜNİVERSİTESİ, ANKARA VETERİNER FAKÜLTESİ)0000-0002-5767-583X
Ahu Demirtaş(BURDUR MEHMET AKİF ERSOY UNIVERSITY)0000-0003-2942-6243
Hakan Öztürk(Ankara University)0000-0003-2913-2069
Sayfa
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Yayın Tarihi
2026-04-01
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Additionally, since lysozyme has limited selectivity for certain rumen bacteria within the Rusitec system, lysozyme may influence rumen microbes differently from conventional antibiotics, warranting further in vitro and in vivo studies across diverse diets and dose ranges.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The aim of the present study was to investigate the in vitro effects of two lysozyme doses (10 and 50 FIP U/mL) on rumen fermentation and microbial populations using the artificial rumen system Rusitec with an 80:20 concentrate-to-forage diet.
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Temel Bulgular & Veri:The ruminal pH, the total number of protozoa, total and individual volatile fatty acid (VFA) production, and methane (CH4) release were not significantly affected by the tested quantities of lysozyme.
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Sonuç & Katkı:Additionally, since lysozyme has limited selectivity for certain rumen bacteria within the Rusitec system, lysozyme may influence rumen microbes differently from conventional antibiotics, warranting further in vitro and in vivo studies across diverse diets and dose ranges.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Enzymes · feed additives · rumen bacteria

Abstract

The aim of the present study was to investigate the in vitro effects of two lysozyme doses (10 and 50 FIP U/mL) on rumen fermentation and microbial populations using the artificial rumen system Rusitec with an 80:20 concentrate-to-forage diet. The ruminal pH, the total number of protozoa, total and individual volatile fatty acid (VFA) production, and methane (CH4) release were not significantly affected by the tested quantities of lysozyme. However, the ammonia nitrogen (NH3-N) concentration was significantly increased by supplementation with Lysozyme-50 (P < 0.05). The dry matter digestibility (DMD) was significantly higher in Lysozyme-50 than in Lysozyme-10 (P < 0.05). The total bacterial count decreased linearly in response to the addition of Lysozyme-50 (P < 0.05). Furthermore, in the presence of Lysozyme-50, the abundance of Butyrivibrio fibrisolvens decreased in both linear and quadratic manners (P < 0.05), and the abundance of Fibrobacter succinogenes decreased in a linear manner (P < 0.05). A linear decline tendency in the cell numbers of Ruminococcus albus (P = 0.092) and Megasphaera elsdenii (P = 0.085) was observed with Lysozyme-50 treatment. These findings suggest that while core fermentation parameters such as VFA production and digestibility remained stable, the elevated ammonia nitrogen concentration may reflect reduced nitrogen utilization efficiency, particularly at high doses. Additionally, since lysozyme has limited selectivity for certain rumen bacteria within the Rusitec system, lysozyme may influence rumen microbes differently from conventional antibiotics, warranting further in vitro and in vivo studies across diverse diets and dose ranges.

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Bu Makaleye Atıf Yap

Musa, S. A. A., Demirtaş, A., Öztürk, H. (2026). Impact of lysozyme on ruminal fermentation and microbial population in Rusitec fermenters. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Volume: 73 Issue: 2. https://doi.org/10.33988/auvfd.1725307

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Kaynakça (54)

  1. Abdl-Rahman MA (2010): In vitro manipulation of rumen fermentation efficiency by fumaric acid-bentonite coupled addition as an alternative to antibiotics. J Agric Sci, 2, 174-180.
  2. Adduci F, Elshafie HS, Labella C, et al (2019): Abatement of the clostridial load in the teats of lactating cows with lysozyme derived from donkey milk. J Dairy Sci, 102, 6750-6755.
  3. AOAC (Association of Official Analytical Chemists) (2000): Official Methods of Analysis of AOAC International. 17th ed. AOAC, USA.
  4. Aratboni HA, Olvera C, Ayala M (2024): Nanoformulations for lysozyme-based additives in animal feed: An alternative to fight antibiotic resistance spread. Nanotechnol Rev, 13, 20240015.
  5. Attwood GT, Klieve AV, Ouwerkerk D, et al (1998): Ammonia-hyperproducing bacteria from New Zealand ruminants. Appl Environ Microb, 64, 1796-804.
  6. Benhissi H, de Heredia, IB, García-Rodríguez A (2016): Effect of replacing palm fat with high-linoleic cold-pressed rapeseed or sunflower cakes on fatty acid biohydrogenation in an artificial rumen (Rusitec). Anim Prod Sci, 58, 499-506.
  7. Biswas AA, Lee SS, Mamuad LL, et al (2016): Use of lysozyme as a feed additive on in vitro rumen fermentation and methane emission. Asian-Australas J Anim Sci, 29, 1601-1607.
  8. Boguhn J, Kluth H, Rodehutscord M (2006): Effect of total mixed ration composition on fermentation and efficiency of ruminal microbial crude protein synthesis in vitro. J Dairy Sci, 89, 1580-1591.
  9. Busquet M, Calsamiglia S, Ferret A, et al (2005): Effect of garlic oil and four of its compounds on rumen microbial fermentation. J Dairy Sci, 88, 4393-4404.
  10. Chaney AL, Marbach EP (1962): Modified reagents for determination of urea and ammonia. Clin Chem, 8, 130-132.
  11. Czerkawski JW, Breckenridge, G (1977): Design and development of a long-term rumen simulation technique (RUSITEC). Br J Nutr, 38, 371-384.
  12. Demirtas A, Ozturk H, Sudagidan M, et al (2019): Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec). Anaerobe, 55, 83-92.
  13. Demirtas A, Sevin S, Musa SAA, et al (2023): Preliminary study of the effects of Apilactobacillus kunkeei EIR/BG-1 and Enterococcus hirae EIR/CM-2 supplementation on in vitro rumen fermentation and microbial population. J Anim Feed Sci, 33, 88-100.
  14. Denman SE, McSweeney CS (2006): Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiol Ecol, 58, 572-582.
  15. Denman SE, Tomkins NW, McSweeney CS (2007): Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane. FEMS Microbiol Ecol, 62, 313-322.
  16. Donald EJ, Ward GM (1996): Estimates of animal methane emissions. Environ Monit Assess, 42, 133-141.
  17. Duarte AC, Holman DB, Alexander TW, et al (2017): Incubation temperature, but not pequi oil supplementation, affects methane production, and the ruminal microbiota in a rumen simulation technique (Rusitec) system. Front Microbiol, 8, 1076.
  18. El-Deep MH, Amber KA, Eid YZ, et al (2021): The influence of chicken egg lysozyme or zinc-bacitracin antibiotic on the growth performance, antibacterial capacity, blood profiles, and antioxidative status of rabbits: A comparative study. Animals, 11, 1731.
  19. Ellison R, Giehl TJ (1991): Killing of gram-negative bacteria by lactoferrin and lysozyme. J Clin Invest, 88, 1080-1091.
  20. Ferraboschi P, Ciceri S, Grisenti P (2021): Applications of lysozyme, an innate immune defense factor, as an alternative antibiotic. Antibiotics, 10, 1534.
  21. Goiri I, Garcia-Rodriguez A, Oregui LM (2009): Effect of chitosan on mixed ruminal microorganism fermentation using the rumen simulation technique (Rusitec). Anim Feed Sci Tech, 152, 92-102.
  22. Gong M, Anderson D, Rathgeber B, et al (2017): The effect of dietary lysozyme with EDTA on growth performance and intestinal microbiota of broiler chickens in each period of the growth cycle. J Appl Poult Res, 26, 1-8.
  23. Hristov AN, Firkins JL, Dijkstra J, et al (2013): Special topics-mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options. J Anim Sci, 91, 5045-5069.
  24. Ibrahim HR, Kato A, Kobayashi K (1991): Antimicrobial effects of lysozyme against gram-negative bacteria due to covalent binding of palmitic acid. J Agric Food Chem, 39, 2077-2082.
  25. Ibrahim HR, Matsuzaki T, Aoki T (2001): Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function. FEBS Letters, 506, 27-32.
  26. Khafipour E, Li S, Plaizier JC, et al (2009): Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Appl Environ Microbiol, 75, 7115-7124.
  27. Klieve AV, Hennessy D, Ouwerkerk D, et al (2003): Establishing populations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fed high grain diets. J Appl Microbiol, 95, 621-630.
  28. Kohn R, Dinneen M, Russek-Cohen E (2005): Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle, sheep, goats, horses, pigs, and rats. J Anim Sci, 83, 879-889.
  29. Li X, Durmic Z, Liu S, et al (2014): Eremophila glabra reduces methane production and methanogen populations when fermented in a Rusitec. Anaerobe, 29, 100-107.
  30. Long Y, Lin S, Zhu J, et al (2016): Effects of dietary lysozyme levels on growth performance, intestinal morphology, non-specific immunity and mRNA expression in weanling piglets. Anim Sci J, 87, 411-418.
  31. López-García A, Saborío-Montero A, Gutiérrez-Rivas M, et al (2022): Fungal and ciliate protozoa are the main rumen microbes associated with methane emissions in dairy cattle. GigaScience, 11, giab088.
  32. Małaczewska J, Kaczorek-Łukowska E, Wójcik R, et al (2019): Antiviral effects of nisin, lysozyme, lactoferrin and their mixtures against bovine viral diarrhoea virus. BMC Vet Res, 15, 1-12.
  33. Masschalck B, Michiels CW (2003): Antimicrobial properties of lysozyme in relation to foodborne vegetative bacteria. Crit Rev Microbiol, 29, 191-214.
  34. McDougall EI (1948): Studies on ruminant saliva. 1. The composition and output of sheep’s saliva. Biochem J, 43, 99-109.
  35. Newbold CJ, Ramos-Morales E, O'Hara E (2015): Rumen protozoa and methanogenesis: Not a simple cause–effect relationship. Brit J Nutr, 114, 374-377.
  36. Ouwerkerk D, Klieve AV, Forster RJ (2002): Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay. J Appl Microbiol, 92, 753-758.
  37. Öztürk H (2003): In vitro studien zum einfluss von topinamburmehl und Saccharomyces boulardii auf den mikrobiellen vormagenstoffwechsel. Dissertation, Tierärztliche Hochschule Hannover, Germany.
  38. Pellegrini A, Thomas U, Von Fellenberg R, et al (1992): Bactericidal activities of lysozyme and aprotinin against Gram‐negative and Gram‐positive bacteria related to their basic character. J Appl Microbiol, 72, 180-187.
  39. Ragland SA, Criss AK (2017): From bacterial killing to immune modulation: Recent insights into the functions of lysozyme. PLoS Pathog, 13, e1006512.
  40. Rathi CL, Risbud SP, Manohar BM (2008): Novel compositions comprising lysozyme and serratiopeptidase for prevention and treatment of mastitis and metritis. Patent No. WO2008035370. Available at https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2008035370&form=MG0AV3. (Accessed July 14, 2025).
  41. Sahoo NR, Kumar P, Bhusan B, et al (2012): Lysozyme in livestock: A guide to selection for disease resistance: A review. J Anim Prod. Adv, 2, 347-360.
  42. Shi Y, Weimer P (1992): Response surface analysis of the effects of pH and dilution rate on Ruminococcus flavefaciens FD-1 in cellulose-fed continuous culture. Appl Environ Microbiol, 58, 2583-2591.
  43. Smulski S, Gehrke M, Libera K, et al (2020): Effects of various mastitis treatments on the reproductive performance of cows. BMC Vet Res, 16, 1-10.
  44. Tajima K, Aminov RI, Nagamine T, et al (2001): Diet dependent shifts in the bacterial population of the rumen revealed with Real- Time PCR. Appl Environ Microbiol, 67, 2766-2774.
  45. Uğurlu M, Uysal A, Nacar B, et al (2025): Effect of breed and fattening system on fattening performance, rumen and blood parameters in Akkaraman, Karayaka and Herik lambs under indoor conditions. Ankara Univ Vet Fak Derg, 72, 397-404.
  46. Verlhac V (2017): Feed additives for aquatic animals comprising essential oils and lysozyme. Patent No. WO2017064092. Available at https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2017064092&form=MG0AV3. (Accessed July 14, 2025).
  47. Vieco-Saiz N, Belguesmia Y, Raspoet R, et al (2019): Benefits and inputs from lactic acid bacteria and their bacteriocins as alternatives to antibiotic growth promoters during food-animal production. Front Microbiol, 10, 57.
  48. Vilcacundo R, Méndez P, Reyes W, et al (2018): Antibacterial activity of hen egg white lysozyme denatured by thermal and chemical treatments. Sci Pharm, 86, 48.
  49. Watanabe Y, Suzuki R, Koike S, et al (2010): In vitro evaluation of cashew nut shell liquid as a methane-inhibiting and propionate-enhancing agent for ruminants. J Dairy Sci, 93, 5258-5267.
  50. Wells JE, Berry ED, Kalchayanand N, et al (2015): Effect of lysozyme or antibiotics on faecal zoonotic pathogens in nursery pigs. J Appl Microbiol, 118, 1489-1497.
  51. Wolin MJ (1960): A theoretical rumen fermentation balance. J Dairy Sci, 43, 1452-1459.
  52. Xia Y, Kong J, Zhang G, et al (2019): Effects of dietary supplementation with lysozyme on the structure and function of the cecal microbiota in broiler chickens. PLoS One, 14, e0216748.
  53. Yousefi M, Vatnikov YA, Kulikov EV, et al (2020): The protective effects of dietary garlic on common carp (Cyprinus carpio) exposed to ambient ammonia toxicity. Aquaculture, 526, 735400.
  54. Zou L, Xiong X, Liu H, et al (2019): Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs. J Sci Food Agric, 99, 1643-1650.