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Dergiler / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2019 / Cilt: 9 Sayı: 2

Akut İmidacloprid Maruziyetinde Tatlı Su Midyelerinin Bazı Biyokimyasal Yanıtlarındaki Değişimler

Alterations in Some Biochemical Responses of Freshwater Mussels in Acute Imidacloprid Exposure

Ertan Yoloğlu(ADIYAMAN UNIVERSITY, FACULTY OF EDUCATION)0000-0002-9730-9471
Sayfa
213–229
Yayın Tarihi
2019-12-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bu çalışmadan elde edilen veriler, akut imidacloprid maruziyetinin midyelerde oksidatif stres ile ilgili biyokimyasal değişikliklere neden olduğunu göstermiştir.

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Amaç & Hipotez:İmidacloprid yaygın olarak kullanılan bir sistemik neonikotinoid insektisittir.
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Temel Bulgular & Veri:Bu çalışmanın amacı 96 saat süre ile imidaclopridin öldürücü olmayan konsantrasyonlarına (1, 10, 100 ve 1000 µg AI L -1 ) maruz kalan tatlı su midyelerinde ( Unio mancus ) bu insektisitin potansiyel zararlı etkilerini belirlemektir.
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Sonuç & Katkı:Bu çalışmadan elde edilen veriler, akut imidacloprid maruziyetinin midyelerde oksidatif stres ile ilgili biyokimyasal değişikliklere neden olduğunu göstermiştir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:İmidacloprid · Oksidatif stres · Tatlı su midyesi

Özet

İmidacloprid yaygın olarak kullanılan bir sistemik neonikotinoid insektisittir. Bu çalışmanın amacı 96 saat süre ile imidaclopridin öldürücü olmayan konsantrasyonlarına (1, 10, 100 ve 1000 µg AI L -1 ) maruz kalan tatlı su midyelerinde ( Unio mancus ) bu insektisitin potansiyel zararlı etkilerini belirlemektir. Bu amaçla, tatlı su midyelerinin solungaçlarında ve sindirim bezlerinde biyokimyasal belirteçler olarak glutatyon s-transferaz (GST) aktivitesi, karboksilesteraz (CaE) aktivitesi, asetilkolinesteraz (AChE) aktivitesi, glutatyon redüktaz (GR) aktivitesi, redükte glutatyon (GSH) seviyesi ve malondialdehit (MDA) seviyesi değerlendirilmiştir. Test sularındaki gerçek imidacloprid konsantrasyonları LC-MS/MS analizi ile belirlenmiştir. Sonuçlar, sindirim bezlerindeki AChE, GR ve CaE aktivitelerinin farklı maruziyet konsantrasyonlarında anlamlı olarak azaldığını, MDA seviyesinin en yüksek maruziyet konsantrasyonunda anlamlı şekilde arttığını göstermiştir ( p < 0.05). Solungaçlardaki sonuçlar göz önüne alındığında, en düşük maruziyet konsantrasyonu AChE aktivitesinde inhibisyona neden olurken, en yüksek maruziyet konsantrasyonu GST aktivitesinde, GSH seviyesinde ve MDA seviyesinde önemli indüksiyona neden olmuştur ( p < 0.05). Ayrıca, ölçülen imidacloprid konsantrasyonlarının nominal imidacloprid konsantrasyonlarının yaklaşık % 80'i düzeyinde olduğu belirlenmiştir. Bu çalışmadan elde edilen veriler, akut imidacloprid maruziyetinin midyelerde oksidatif stres ile ilgili biyokimyasal değişikliklere neden olduğunu göstermiştir.

Abstract

Imidacloprid is a widely used systemic neonicotinoid insecticide. The aim of this study is to identify potential harmful effects of imidacloprid in freshwater mussels ( Unio mancus ) which were exposed to sub-lethal concentrations ( 1, 10, 100, and 1000 µg AI L -1 ) for 96 h. For this aim, glutathione s-transferase (GST) activity, carboxylesterase (CaE) activity, acetylcholinesterase (AChE) activity, glutathione reductase (GR) activity, reduced glutathione (GSH) level, and malondialdehyde (MDA) level were evaluated as biochemical markers of exposure in the gills and digestive glands. The actual imidacloprid concentrations in the test waters were determined by LC-MS/MS analysis. The results showed that the AChE, GR, and CaE activities in the digestive glands significantly decreased at different exposure concentrations, while the MDA level significantly increased at the highest exposure concentration ( p < 0.05). Considering the results in the gills, the lowest exposure concentration caused inhibition in AChE activity, while the highest exposure concentration caused significant induction in GST activity, GSH level and MDA level ( p < 0.05). In addition, the measured imidacloprid concentrations were determined to be about 80% of the nominal imidacloprid concentrations. The data obtained from this study indicated that acute imidacloprid exposure caused biochemical alterations related to oxidative stress in mussels.

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Bu Makaleye Atıf Yap

Yoloğlu, E. (2019). Akut İmidacloprid Maruziyetinde Tatlı Su Midyelerinin Bazı Biyokimyasal Yanıtlarındaki Değişimler. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 9 Sayı: 2, 213–229. https://doi.org/10.37094/adyujsci.563239

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