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Dergiler / Ziraat Fakültesi Dergisi / 2026 / Sayı: 1

Farklı Uygulama Yaklaşımlarıyla Yerel Beauveria bassiana Kültür Filtratının Domateste Meloidogyne incognita'ya Karşı Etkinliği

Effectiveness of Local Beauveria bassiana Culture Filtrate Against Meloidogyne incognita in Tomato with Different Application Approaches

Fatma Gül Göze Özdemir(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ)0000-0003-1969-4041
Asiye Uzun Yiğit(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ)0000-0002-4822-4762
Ozan Demirözer(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ)0000-0001-7240-8898
Harun Çimenkaya(ISPARTA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ)0009-0001-3211-2045
Sayfa
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Yayın Tarihi
2026-08-27
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Yerel B. bassiana BIM-001 izolatından elde edilen kültür filtratının çoklu kullanımı, Türkiye'de kullanılan tescilli biyonematisit (P. lilacinus suş PL1) kadar etkili nematod öldürücü aktivite sergiledi.

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Amaç & Hipotez:Bu çalışmada, yerel Beauveria bassiana BIM-001 izolatının kültür filtratının, farklı uygulama yöntemleriyle birlikte, kök ur nematodlarıyla önceden enfekte edilmiş toprakta yetiştirilen domatesler üzerindeki etkileri incelenmiştir.
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Temel Bulgular & Veri:Çalışmada, tekli ve çoklu uygulamaları içeren on beş farklı uygulama ve iki kontrol grubu kullanılmıştır.
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Sonuç & Katkı:Yerel B. bassiana BIM-001 izolatından elde edilen kültür filtratının çoklu kullanımı, Türkiye'de kullanılan tescilli biyonematisit (P. lilacinus suş PL1) kadar etkili nematod öldürücü aktivite sergiledi.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Kök ur nematodu · Biyolojik kontrol · Beauveria bassiana

Özet

Bu çalışmada, yerel Beauveria bassiana BIM-001 izolatının kültür filtratının, farklı uygulama yöntemleriyle birlikte, kök ur nematodlarıyla önceden enfekte edilmiş toprakta yetiştirilen domatesler üzerindeki etkileri incelenmiştir. Çalışmada, tekli ve çoklu uygulamaları içeren on beş farklı uygulama ve iki kontrol grubu kullanılmıştır. Ur indeksi, uygulamalar arasında önemli ölçüde farklılık göstermiş ve 1.6 ile 4.7/0-5 arasında değişmiştir. Negatif kontrol grubunda (damıtılmış su) 4.7 belirlenirken, pozitif kontrol grubunda (Paecilomyces lilacinus PL1 suşu) 2.1 bulunmuştur. Çoklu uygulamaların kontrol etkisi, tekli uygulamaya göre önemli ölçüde daha yüksek olmuştur. Kültür filtratının tekli kullanımında, ekimden 15 gün önce yapılan uygulama, 2.5 ur indeksi ile daha etkili olmuştur. En düşük ur indeksi, ekimden 15 gün önce başlayan çoklu kullanımda gözlemlendi: 1.8 (ekimden 15 gün önce ve 3 gün sonra toprağa uygulama), 1.7 (ekimden 15 gün önce ve 15 gün sonra toprağa uygulama) ve 1.6 (ekimden 15 gün önce ve 1 gün önce toprağa uygulama). Yerel B. bassiana BIM-001 izolatından elde edilen kültür filtratının çoklu kullanımı, Türkiye'de kullanılan tescilli biyonematisit (P. lilacinus suş PL1) kadar etkili nematod öldürücü aktivite sergiledi.

Abstract

In this study the effects of culture filtrate of the native Beauveria bassiana BIM-001 isolate on tomatoes grown in pre-infested soil with root-knot nematodes in combination with different treatments. Fifteen different treatments involving single and multiple applications, with two controls, were utilized in the study. The gall index ranged from 1.6 to 4.7 (0–5 scale) across treatments. The gall index was 4.7 in the negative control (distilled water) and 2.1 in the positive control (Paecilomyces lilacinus PL1). Multiple treatments were significantly more effective than a single application. With a single application, treatment 15 days before planting was most effective (gall index 2.5). The lowest gall index was observed in the multiple-use starting 15 days before planting: 1.8 (soil application at 15 days before and 3 days after planting), 1.7 (soil application at 15 days before and 15 days after planting), and 1.6 (soil application at 15 days before and 1 day before planting). Multiple applications of the native B. bassiana BIM-001 filtrate was as effective as the registered bionematicide (P. lilacinus strain PL1) used in Türkiye.

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Bu Makaleye Atıf Yap

Özdemir, F. G. G., Yiğit, A. U., Demirözer, O., Çimenkaya, H. (2026). Farklı Uygulama Yaklaşımlarıyla Yerel Beauveria bassiana Kültür Filtratının Domateste Meloidogyne incognita'ya Karşı Etkinliği. Ziraat Fakültesi Dergisi, Sayı: 1. https://doi.org/10.54975/isubuzfd.1852154

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Kaynakça (45)

  1. Abd-Elgawad, M. M. (2021). Optimizing safe approaches to manage plant-parasitic nematodes. Plants, 10(9), 1911. https://doi.org/10.3390/plants10091911
  2. Baazeem, A., Alorabi, M., Manikandan, P., Alotaibi, S. S., Almanea, A., Abdel-Hadi, A., & Kim, H. J. (2021). Paecilomyces formosus MD12, a biocontrol agent to treat Meloidogyne incognita on brinjal in green house. Journal of Fungi, 7(8), 632. https://doi.org/10.3390/jof7080632
  3. Bamisile, B. S., Akutse, K. S., Siddiqui, J. A., & Xu, Y. (2021). Model application of entomopathogenic fungi as alternatives to chemical pesticides: Prospects, challenges, and insights for next-generation sustainable agriculture. Frontiers in Plant Science, 12, 741804. https://doi.org/10.3389/fpls.2021.741804
  4. Barbary, A., Djian‐Caporalino, C., Palloix, A., & Castagnone‐Sereno, P. (2015). Host genetic resistance to root‐knot nematodes, Meloidogyne spp., in Solanaceae: From genes to the field. Pest Management Science, 71(12), 1591-1598. https://doi.org/10.1002/ps.4091
  5. Bridge, J., & Starr, J. L. (2007). Plant Nematodes of Agricultural Importance: A color handbook (CRC Press; San Diego, CA, USA, 2007). https://doiorg/101201/b15142
  6. Brownbridge, M., Reay, S. D., Nelson, T. L., & Glare, T. R. (2012). Persistence of Beauveria bassiana (Ascomycota: Hypocreales) as an endophyte following inoculation of radiata pine seed and seedlings. Biological Control, 61(3), 194-200. https://doi.org/10.1016/j.biocontrol.2012.01.002
  7. Coolen, W. A., & D'herde, C. J. (1972). A method for the quantitative extraction of nematodes from plant tissue. 77 pp.
  8. Damalas, C. A., & Eleftherohorinos, I. G. (2011). Pesticide exposure, safety issues, and risk assessment indicators. International Journal of Environment Research and Public Health, 8(5), 1402–1419. https://doi.org/10.3390/ijerph8051402
  9. Desaeger, J., Wram, C., & Zasada, I. (2020). New reduced-risk agricultural nematicides-rationale and review. Journal of Nematology, 52, e2020-91. https://doi.org/10.21307/jofnem-2020-091
  10. EFSA Scientific Committee, (2014). Scientific Opinion on the essential composition of infant and follow on formulae. EFSA Journal, 12(7), 3760.
  11. Erol, D. (2025). Investigation of the efficacy of some biological preparations against Meloidogyne incognita (Master's thesis, Akdeniz University, Institute of Natural and Applied Sciences, Antalya, Türkiye).
  12. Gisbert, C., Trujillo‐Moya, C., Sánchez‐Torres, P., Sifres, A., Sánchez‐Castro, E., & Nuez, F. (2013). Resistance of pepper germplasm to Meloidogyne incognita. Annals of Applied Biology, 162(1), 110-118. https://doi.org/10.1111/aab.12006
  13. Göze Özdemir, F. G., Uzun, A., & Demirözer, O. (2022). Assessment of nematicidal activity of Beauveria bassiana (Bals.-Criv.) vuill on Pratylenchus thornei (Sher et Allen)(Tylenchida: Pratylenchidae). International Journal of Agriculture Environment and Food Sciences, 6(1), 65-71. https://doi.org/10.31015/jaefs.2022.1.10
  14. Göze Özdemir, F. G., Uzun Yiğit, A., & Demirözer, O. (2023). Evaluation of the entomopathogenic fungi Beauveria bassiana culture filtrate for Meloidogyne incognita on tomato. Journal of Plant Diseases and Protection, 130(2), 271-278. https://doi.org/10.1007/s41348-022-00698-1
  15. Hamill, R. L., Higgens, C. E., Boaz, H. E., & Gorman, M. (1969). The structure of beauvericin, a new depsipeptide antibiotic toxic to Artemia salina. Tetrahedron Letter, 10(49), 4255-4258. https://doi.org/10.1016/S0040-4039(01)88668-8
  16. Hartman, K. M., & Sasser, J. N. (1985). Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. An Advanced Treatise on Meloidogyne, 2, 69-77.
  17. Hooper, D. J., Hallmann, J., & Subbotin, S. A. (2005). Methods for extraction, processing and detection of plant and soil nematodes. In Plant parasitic nematodes in subtropical and tropical agriculture (pp. 53-86). Wallingford UK: CABI Publishing. https://doi.org/10.1079/9780851997278.0053
  18. Hummadi, E. H., Dearden, A., Generalovic, T., Clunie, B., Harrott, A., Cetin, Y., & Butt, T. (2021). Volatile organic compounds of Metarhizium brunneum influence the efficacy of entomopathogenic nematodes in insect control. Biological Control, 155, 104527. https://doi.org/10.1016/j.biocontrol.2020.104527
  19. Kannan, R., & Veeravel, R. (2012). Effect of different dose and application methods of Paecilomyces lilacinus (Thom) Samson against root knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood in okra. Journal of Agricultural Science, 4(11), 119-127. http://dx.doi.org/10.5539/jas.v4n11p119
  20. Kerry, B. R., & Bourne, J. M. (2002). A manual for research on Verticillium chlamydosporium, a potential biological control agent for root-knot nematodes. Gent: IOBC/WPRS.
  21. Khan, M. R., Zaidi, B., & Haque, Z. (2012). Nematicides control rice root-knot, caused by Meloidogyne graminicola. Phytopathologia Mediterranea, 51(2), 298-306.
  22. Khoja, S., Eltayef, K. M., Baxter, I., Myrta, A., Bull, J. C., & Butt, T. (2021). Volatiles of the entomopathogenic fungus, Metarhizium brunneum, attract and kill plant parasitic nematodes. Biological Control, 152, 104472. https://doi.org/10.1016/j.biocontrol.2020.104472
  23. Liu, X. S., Zhang, Z. N., & Huang, Y. (2007). Sublittoral meiofauna with particular reference to nematodes in the southern Yellow Sea, China. Estuarine, Coastal and Shelf Science, 71(3-4), 616-628. https://doi.org/10.1016/j.ecss.2006.09.013
  24. Liu, T., Wang, L., Duan, Y. X., & Wang, X. (2008). Nematicidal activity of culture filtrate of Beauveria bassiana against Meloidogyne hapla. World Journal of Microbiology and Biotechnology, 24, 113-118. https://doi.org/10.1007/s11274-007-9446-z
  25. Lopez-Llorca, L. V., Maciá-Vicente, J. G., & Jansson, H. B. (2008). Mode of action and interactions of nematophagous fungi in integrated management and biocontrol of vegetable and grain crops nematodes (pp 51-76) Dordrecht, Springer Netherlands.
  26. Mayer, A. (1995). Secondary metabolites with nematicidal and antimicrobial activity from nematophagous fungi and ascomycetes (PhD thesis, University of Kaiserslautern, Germany).
  27. Mishra, J., Dutta, V., & Arora, N. K. (2020). Biopesticides in India: technology and sustainability linkages 3. Biotech, 10(5), 210. https://doi.org/10.1007/s13205-020-02192-7
  28. Ownley, B. H., Pereira, R. M., Klingeman, W. E., Quigley, N. B., & Leckie, B. M. (2004). Beauveria bassiana, a dual purpose biocontrol organism, with activity against insect pests and plant pathogens. Eds; R.T. Lartey, A.J. Caesar, Emerging Concepts in Plant Health Management, Research Signpost, Kerala, India, pp. 255-269.
  29. Russo, M. L., Pelizza, S. A., Cabello, M. N., Stenglein, S. A., & Scorsetti, A. C. (2015). Endophytic colonisation of tobacco, corn, wheat and soybeans by the fungal entomopathogen Beauveria bassiana (Ascomycota, Hypocreales). Biocontrol Science and Technology, 25(4), 475-480. https://doi.org/10.1080/09583157.2014.982511
  30. Sánchez-Gómez, T., Harte, S. J., Zamora, P., Bareyre, M., Díez, J. J., Herrero, B., & Martín-García, J. (2023). Nematicidal effect of Beauveria species and the mycotoxin beauvericin against pinewood nematode Bursaphelenchus xylophilus. Frontiers in Forests and Global Change, 6, 1229456. https://doi.org/10.3389/ffgc.2023.1229456
  31. Saragih, M. (2019). Endophytic colonization and plant growth promoting effect by Entomopathogenic fungus, Beauveria bassiana to Red Chili (Capsicum annuum L) with Different Inoculation Methods. In IOP Conference Series: Earth and Environmental Science (Vol 305, No 1, p 012070) IOP Publishing. 10.1088/1755-1315/305/1/012070
  32. Seid, A., Fininsa, C., Mekete, T., Decraemer, W., & Wesemael, W. M. (2015). Tomato (Solanum lycopersicum) and root-knot nematodes (Meloidogyne spp.)–a century-old battle. Nematology, 17(9), 995-1009. https://doi.org/10.1163/15685411-00002935
  33. Seong, J., Shin, J., Kim, K., & Cho, B. K. (2021). Microbial production of nematicidal agents for controlling plant parasitic nematodes. Process Biochemistry, 108, 69–79. https://doiorg/101016/jprocbio202106006
  34. Sharma, I. P., & Sharma, A. K. (2017). Co-inoculation of tomato with an arbuscular mycorrhizal fungus improves plant immunity and reduces root-knot nematode infection. Rhizosphere, 4, 25-28. https://doi.org/10.1016/j.rhisph.2017.05.008
  35. Shimada, A., Fujioka, S., Koshino, H., & Kimura, Y. (2010). Nematicidal activity of beauvericin produced by the fungus Fusarium bulbicola. Zeitschrift für Naturforschung C, 65(3–4), 207–210.
  36. Singh, A., Sharma, P., Kumari, A., Kumar, R., & Pathak, D. V. (2019). Management of root-knot nematode in different crops using microorganisms. In Plant Biotic Interactions: State of the Art (pp. 85-99). Cham: Springer International Publishing, 85-99. https://doi.org/10.1007/978-3-030-26657-8_6.
  37. Subedi, S., Thapa, B., & Shrestha, J. (2020). Root-knot nematode (Meloidogyne incognita) and its management: a review. Journal of Agriculture and Natural Resources, 3(2), 21-31. https://doi.org/10.3126/janr.v3i2.32298
  38. Teillet, A., Dybal, K., Kerry, B. R., Miller, A. J., Curtis, R. H., & Hedden, P. (2013). Transcriptional changes of the root-knot nematode Meloidogyne incognita in response to Arabidopsis thaliana root signals. Public Library of Science One, 8(4), e61259. https://doi.org/10.1371/journal.pone.0061259
  39. Uysal, G., Söğüt, M. A., & Elekçioğlu, İ. H. (2017). Identification and distribution of root-knot nematode species (Meloidogyne spp.) in vegetable growing areas of Lakes Region in Turkey. Turkish Journal of Entomology, 41(1), 105-122. https://doi.org/10.16970/ted.91225
  40. Uzun, A., (2020). Thysanoptera species of the cereal production areas in lakes region, distribution ratios and population fluctuations of important species and molecular characterization of some species of phlaeothripidae. Isparta University of Applied Sciences, The Institute of Graduate Education Department of Plant Protection PhD Thesis, pp. 266.
  41. Van Bael, S. A., Mejía, L. C., Bischoff, J. F., Rojas, E. I., Arnold, A. E., Robbins, N., Herre, E. A., Kyllo, D. A., & Maynard, Z. (2005). Emerging perspectives on the ecological roles of endophytic fungi in tropical plants. In: J Dighton, J White, P Oudemans, eds The fungal community: its organization and the role in the ecosystem London, UK: CRC Press, 181–191.
  42. Vega, F. E. (2008). Insect pathology and fungal endophytes. Journal of invertebrate pathology, 98(3), 277-279. https://doi.org/10.1016/j.jip.2008.01.008
  43. Viggiano, J. R., de Freitas, L. G., & Lopes, E. A. (2014). Use of Pochonia chlamydosporia to control Meloidogyne javanica in cucumber. Biological control, 69, 72-77. https://doi.org/10.1016/j.biocontrol.2013.11.004
  44. Yerukala, S., Bernard, E. C., Gwinn, K. D., Butler, D. M., Grewal, P. S., & Ownley, B. H. (2021). Endophytic Beauveria bassiana increases galling of ‘Rutgers’ tomato roots with Meloidogyne incognita. Journal of Nematology, 53, e2021-72. https://doi.org/10.21307/jofnem-2021-072
  45. Youssef, M. M., El-Nagdi, W. M., & Lotfy, D. E. (2020). Evaluation of the fungal activity of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus as biocontrol agents against root-knot nematode, Meloidogyne incognita on cowpea. Bulletin of the National Research Centre, 44(112), 1-11. https://doi.org/10.1186/s42269-020-00367-z