Dergiler / Tekirdağ Ziraat Fakültesi Dergisi / 2026 / Cilt: 23 Sayı: 5

Kimyasal Mutajenlerin Aspir (Carthamus tinctorius L.)’de Çimlenme ve Fide Parametrelerine Etkisi

Impact of Chemical Mutagens on The Germination and Seedling Parameters in Safflower (Carthamus tinctorius L.)

Tansu Uskutoğlu(Pamukkale Üniversitesi, Ziraat Fakültesi)0000-0001-6631-1723
Fatih Kıllı(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARLA BİTKİLERİ BÖLÜMÜ, TARLA BİTKİLERİ PR.)0000-0001-8480-0416
Cüneyt Cesur(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARLA BİTKİLERİ BÖLÜMÜ, TARLA BİTKİLERİ PR.)0000-0002-1607-363X
Sayfa
1570–1583
Yayın Tarihi
2026-10-01
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

En uzun fide boyu (32.0 cm) ve kök uzunluğu (14.9 cm) parametrelerinin her ikisi de 2 saat süreyle uygulanan %0.2 SA mutagen uygulamasında gözlenmiş olup, düşük doz ve kısa süreli SA uygulamalarının üstün fide gelişimini teşvik ettiğini göstermektedir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Aspir (Carthamus tinctorius L.) diğer yağ bitkilerine göre ihmal edilmiş bir bitki olmasına rağmen, insan tüketimi için yağ üretimi, halk hekimliğinde tıbbi uygulamalar, boya üretimi (sarı ve kırmızı), hayvan yemi, çay (taç yapraklarından) ve kesme çiçek olarak çeşitli amaçlarla kullanılmaktadır.
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Temel Bulgular & Veri:Bu çalışma, farklı etki mekanizmalarına sahip iki kimyasal mutajenin, alkilleyici ajan (EMS) ve solunum inhibitörünün (SA), aspir tohumları üzerindeki etkilerini inceleme için yürütülmüştür.
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Sonuç & Katkı:En uzun fide boyu (32.0 cm) ve kök uzunluğu (14.9 cm) parametrelerinin her ikisi de 2 saat süreyle uygulanan %0.2 SA mutagen uygulamasında gözlenmiş olup, düşük doz ve kısa süreli SA uygulamalarının üstün fide gelişimini teşvik ettiğini göstermektedir.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Aspir · Çimlenme · EMS

Özet

Aspir (Carthamus tinctorius L.) diğer yağ bitkilerine göre ihmal edilmiş bir bitki olmasına rağmen, insan tüketimi için yağ üretimi, halk hekimliğinde tıbbi uygulamalar, boya üretimi (sarı ve kırmızı), hayvan yemi, çay (taç yapraklarından) ve kesme çiçek olarak çeşitli amaçlarla kullanılmaktadır. Bu çalışma, farklı etki mekanizmalarına sahip iki kimyasal mutajenin, alkilleyici ajan (EMS) ve solunum inhibitörünün (SA), aspir tohumları üzerindeki etkilerini inceleme için yürütülmüştür. Çalışmada materyal olarak yerel aspir çeşidi olan "Linas" kullanılmıştır. Kimyasal mutajenler farklı konsantrasyonlarda (%0.2, %0.3 ve %0.4) ve farklı sürelerde (2 saat, 4 saat ve 6 saat) uygulanmıştır. Fide özellikleri tesadüf parselleri deneme deseninde 4 tekrarlı olarak, iklim odasında kontrollü şartlarda gerçekleştirilmiştir. Çimlenme yüzdesi, daha yüksek konsantrasyonlar ve daha uzun uygulama sürelerinde azalmıştır. Ayrıca, çimlenme ve fide özellikleri daha yüksek konsantrasyonlar ve daha uzun uygulama sürelerinden olumsuz etkilendiği belirlenmiştir. En uzun fide boyu (32.0 cm) ve kök uzunluğu (14.9 cm) parametrelerinin her ikisi de 2 saat süreyle uygulanan %0.2 SA mutagen uygulamasında gözlenmiş olup, düşük doz ve kısa süreli SA uygulamalarının üstün fide gelişimini teşvik ettiğini göstermektedir. En yüksek fide yaş ağırlığı (3.362 mg) 2 saatlik %0.4 SA uygulamasında elde edilirken; en yüksek kuru ağırlık (%7.5) ise 2 saatlik %0.2 EMS uygulamasında belirlenmiştir. SA uygulamaları, özellikle düşük dozlarda ve orta süreli uygulamalarda, EMS ve kontrol gruplarına kıyasla daha fazla yaprak sayısı, yaprak kuru ağırlığı ve kök biyokütlesi ile sonuçlanmıştır. Aspir tohumları, SA uygulamasına kıyasla EMS uygulamasından daha olumsuz etkilenmiştir. SA uygulamasında ilk çıkış gecikmiş olmasına rağmen, bazı fideler kontrole kıyasla üstün özellikler sergilediği görülmüştür. Öte yandan, EMS uygulamasında gözlenen çimlenme ve fide parametrelerindeki azalma, tohumların EMS tarafından daha ciddi şekilde hasar gördüğünü ve böylece daha büyük varyasyonların ortaya çıkabileceğini göstermektedir. İncelenen tüm özellikler için, her iki mutajen de aspir çimlenmesi ve fide parametrelerinde varyasyon oluşturmuş olup, her iki mutajeninde aspir mutasyon ıslahı programlarında kullanılabileceğini göstermektedir. Islah amaçları doğrultusunda, %0.2 SA uygulaması (2–4 saat) fide büyümesini ve yaprak gelişimini optimize ederken; %0.4 SA (4 saat) ve %0.2 EMS (6 saat) uygulamaları fizyolojik varyasyonu indüklemede en etkili yöntemler olmuştur.

Abstract

The safflower (Carthamus tinctorius L.) plant is a neglected species; however, it can be used for various purposes, such as oil production for human consumption, medicinal applications in folk medicine, dye production (yellow and red), fodder, tea (from petals), and as a cut flower. The aim of this study was to examine the effects of two chemical mutagens with different mechanisms of action, an alkylating agent (EMS) and a respiratory inhibitor (SA) on safflower seeds. The local safflower variety “Linas” was used in the experiments. Chemical mutagens were applied at different concentrations (0.2%, 0.3%, and 0.4%) and for different durations (2 h, 4 h, and 6 h). The experiment was conducted in a growth chamber under controlled conditions, following a completely randomized design with four replicates to determine seedling parameters. Germination percentage decreased with higher concentrations and longer application durations. Additionally, germination and seedling characteristics were negatively affected by higher concentrations and longer application durations. The longest seedling length (32.0 cm) and root length (14.9 cm) were both observed with the 0.2% SA treatment applied for 2 hours, indicating that low-dose and short-duration SA applications promote superior seedling growth. The maximum seedling fresh weight (3,362 mg) was achieved with the 0.4% SA treatment for 2 h, whereas the highest dry weight (7.5%) was recorded under the 0.2% EMS treatment for 2 h. SA treatments resulted in higher leaf number, leaf dry weight, and root biomass compared to EMS and control, especially at lower doses and moderate application times. Safflower seeds were more adversely affected by EMS treatment than by SA treatment. Although the initial emergence was delayed in the SA treatment, some seedlings exhibited superior characteristics compared to the control. On the other hand, the reduction in germination and seedling parameters observed in the EMS treatment indicated that the seeds were more severely damaged by EMS, and thus, greater variation may occur. For all traits examined, both mutagens induced variation in safflower germination and seedling parameters, indicating that both can be used in safflower mutation breeding programs. The 0.2% SA treatment (2–4 h) optimized seedling growth and leaf development for breeding purposes, while 0.4% SA (4 h) and 0.2% EMS (6 h) were most effective for inducing physiological variation.

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Bu Makaleye Atıf Yap

Uskutoğlu, T., Kıllı, F., Cesur, C. (2026). Kimyasal Mutajenlerin Aspir (Carthamus tinctorius L.)’de Çimlenme ve Fide Parametrelerine Etkisi. Tekirdağ Ziraat Fakültesi Dergisi, Cilt: 23 Sayı: 5, 1570–1583. https://doi.org/10.33462/jotaf.1692925

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Kaynakça (67)

  1. Akbay, G., Çiftçi Y. C. and Ünver, S. (1995). Effect of EMS (ethylmethane sulphonate) applied at different doses, durations, and temperatures on some characteristics of M1 plants in barley cultivar Tokak 157/37. Anadolu Journal of Aegean Agricultural Research Institute, 5(1): 9-19. (In Turkish)
  2. Alabd Alhadi, R. and Aabou-Isba, S. M. (2022). Effect of different concentrations of sodium azide on some traits of barley (Hordeum vulgare L.). Acta Scientific Agriculture, 6(8): 04-08.
  3. Alaca, F. and Arslan, N. (2012). The importance of secondary metabolites for plants. Ziraat Mühendisliği, 358: 48-55.
  4. Ali, F., Nadeem, M. A., Habyarimana, E., Yılmaz, A., Nawaz, M. A., Khalil, I. H., Ercisli, S., Chung, G., Chung, G., Chaudhary, H. J. and Bolach, F. S. (2020). Molecular characterization of genetic diversity and similarity centers of safflower accessions with ISSR markers. Brazilian Journal of Botany, 43: 109–121.
  5. Al-Nuaimi, F. K. G. and Al-Shamma, L. M. J. (2015). Effect of chemical mutagens on some morphological traits of Vicia faba L. Cv. Aqadulce. Iraqi Journal of Science, 56(3C): 2506-2512.
  6. Ananthaswamy, H.M., Vakil, V.K. and Shrinivas, A. (1971). Biological and physiological changes in gamma irradiated wheat during germination. Radiation Botany, 11: 1-12.
  7. Anonymous (2018). Manual on Mutation Breeding. International Atomic Energy Agency, Vienna, Austria. Bahar, B. and Akkaya, M. S. (2009). Effects of EMS treatment on the seed germination in wheat. Journal of Applied Biological Sciences, 3(1): 59-64.
  8. Cabahug, R. A. M., Thai Ha, M. K. T., Lim, K. and Hwang, Y. (2020). LD50 determination and phenotypic evaluation of three Echeveria varieties induced by chemical mutagens. Toxicology and Environmental Health Sciences, 12: 1-9.
  9. Chrispeeds, M. J. and Varner, J. E. (1976). Gibberelic acid induced synthesis and release of α-amylase and ribonuclease by isolated barley aleurons layers. Plant Physiology, 42: 346-406.
  10. Croft, H. and Chen, J.M. (2017). Leaf Pigment Content. In: Reference Module in Earth Systems and Environmental Sciences, Ed(s): Elias, S., Oxford, Elsevier Inc, U. K.
  11. Culpan, E. and Arslan B. (2022). Determination of some morphological and technological characters in F2 generation of oleic and mid-oleic type safflower genotypes (Carthamus tinctorius L.). Journal of Tekirdag Agricultural Faculty, 19(1): 156-165. (In Turkish)
  12. Datta, A. K. and Biswas, A. K. (1985). Induced mutagenesis in Nigella sativa L. Cytologia 50(3): 545-562.
  13. Degano, I., Łucejko, J. J. and Colombini, M. P. (2011). The unprecedented identification of safflower dyestuff in a 16th century tapestry through the application of a new reliable diagnostic procedure. Journal of Cultural Heritage, 12(3): 295-299.
  14. Deshpande, A. S. andMalode, S. N. (2019). Effect of sodium azide and ethyl methane sulphonate on seed germination, seedling height and pollen fertility in Linum usitatissimum var. Pkv Nl 260. International E- Research Journal, 110(E): 7-16.
  15. Dhakshanamoorthy, D., Selvaraj, R. and Chidambaram, A. (2010). Physical and chemical mutagenesis in Jatropha curcas L. to induce variability in seed germination, growth and yield traits. Romanian Journal of Biology-Plant Biology, 55(2): 113–125.
  16. Dwivedi, S. L., Upadhyaya, H. D. and Hegde, D. M. (2005). Development of core collection using geographic information and morphological descriptors in safflower (Carthamus tinctorius L.) germplasm. Genetic Resources and Crop Evolution, 52: 821-830.
  17. El-Katony, T. M., Deyab, M. A., El-Adl, M. F. and Ward, F. M. E. N. (2021). Extracts of the brown alga Dictyota dichotoma (Hudson) JV Lamouroux alleviate salt stress in rice (Oryza sativa L.) during germination. Journal of Plant Growth Regulation, 40: 986-999.
  18. Emrani, S. N., Arzani, A. and Saeidi, G. (2011). Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens. African Journal of Biotechnology, 10(59): 12602-12613.
  19. Genc, I. and Yagbasanlar, T. (1994). Plant Breeding. Çukurova University, Faculty of Agriculture, Field Crops Department, Adana, Türkiye. (In Turksih)
  20. Gunasekaran, A. and Pavadai, P. (2015). Studies on induced physical and chemical mutagenesis in groundnut (Arachis hypogia). International Letters of Natural Sciences, 35: 25-35.
  21. Gyulai, J. (1996). Market Outlook for Safflower. North American Safflower Conference. 17-18 January, P. 15, Alberta, Canada.
  22. Hefny, Y. A. M., Mohamed, N. E., Abdelraoof, A. O. and Sleem, H. A. (2020). Germination parameters of some bread wheat genotypes under drought stress conditions. SVU-International Journal of Agricultural Sciences, 2(2): 226-241.
  23. Holme, I. B., Gregersen, P. L. and Brinch-Pedersen, H. (2019). Induced genetic variation in crop plants by random or targeted mutagenesis: convergence and differences. Frontiers in Plant Science, 10: 1468.
  24. Huang, W., Ratkowsky, D. A., Hui, C., Wang, P., Su, J. and Shi, P. (2019). Leaf fresh weight versus dry weight: which is better for describing the scaling relationship between leaf biomass and leaf area for broad-leaved plants? Forests, 10(3): 256.
  25. Jayasankar, S., Babu, L. C., Sudhakara, K. and Dhanesh, K. P. (1999). Evaluation of provenances for seedling attributes in teak (Tectona grandis Linn. F.). Silvae Genetica, 48: 115-122.
  26. Kangarsu, S., Ganesharm, S. and Joel, A. J. (2014). Determination of lethal dose for gamma rays and ethyl methane sulfonate induced mutagen1esis in cassava (Manihot esculenta Crantz). International Journal of Scientific Research, 3(1): 77-79.
  27. Kaur, D. and Kaur, A. (2023). Effect of chemical mutagens on seed germination and seedling traits of Jamun. Biological Forum – An International Journal, 15(2): 454-460.
  28. Kaya, M. D., Bayramin, S., Kayaçetin, F., Katar, D. and Şenay, A. (2009). Determination of the gamma (60Co) dose that can be used to create variation in safflower (Carthamus tinctorius L.). Journal of Agriculture Faculty, 4(2): 28–33.
  29. Khan, S., Al-Qurainy, F. and Anwar, F. (2009). Sodium azide: a chemical mutagen for enhancement of agronomic traits of crop plants. Environment and We: An International Journal for Science and Technology, 4: 1-21.
  30. Khan, S. and Goyal, S. (2009). Improvement of mung bean varieties through induced mutations. African Journal of Plant Science, 3: 174-180.
  31. Khan, S., Vaishali and Sharma, V. (2010). Genetic differentiation and diversity analysis of medicinal tree Syzygium cumini (Myrtaceae) from ecologically different regions of India. Physiology and Molecular Biology of Plants, 16: 149–158.
  32. Kleinhofs, A., Owais, W. Μ. and Nilan, R. A. (1978). Azide; Mutation Research, 55: 165–195.
  33. Kumar, G. and Rai, P. K. (2007). EMS induced karyomorphological variations in maize (Zea mays L.) inbreds. Turkish Journal of Biology, 31(4): 187-195.
  34. Kumar, G. and Yadav, R. S. (2010). EMS induced genomic disorders in sesame (Sesamum indicum L.). Romanian Journal of Biology-Plant Biology, 55(2): 97-104.
  35. Kumar, S., Ambreen, H., Murali, T. V., Bali, S., Agarwal, M., Kumar, A. et al. (2015). Assessment of genetic diversity and population structure in a global reference collection of 531 accessions of Carthamus tinctorius L. (Safflower) using AFLP markers. Plant Molecular Biology Reporter, 33: 1299-1313.
  36. Kumar, S., Ambreen, H., Variath, M. T., Rao, A. R., Agarwal, M., Kumar A. et al. (2016). Utilization of molecular, phenotypic, and geographical diversity to develop compact composite core collection in the oilseed crop, safflower (Carthamus tinctorius L.) through maximization strategy. Frontiers in Plant Science, 7: 1554.
  37. Kumari, V., Chaudhary, H. K., Prasad, R., Kumar, A., Singh, A. et al. (2016). Effect of mutagenesis on germination, growth and fertility in sesame (Sesamum indicum L.). Annual Research & Review in Biology, 10(6): 1-9.
  38. La Bella, S., Tuttolomondo, T., Lazzeri, L., Matteo, R., Leto, C. and Licata, M. (2019). An agronomic evaluation of new safflower (Carthamus tinctorius L.) germplasm for seed and oil yields under Mediterranean climate conditions. Agronomy, 9(8): 468. https://doi.org/10.3390/agronomy9080468
  39. Mensah, J. K., Obadoni, B. O., Akomeah, P. A., Ikhajiagbe, B. and Ajibolu, J. (2007). The effects of sodium azide and colchicine treatments on morphological and yield traits of sesame seed (Sesame indicum L.). African Journal of Biotechnology, 6(5): 534-538.
  40. Mirmazloum, I., Kiss, A., Erdélyi, É., Ladányi, M., Németh, É. Z. and Radácsi, P. (2020). The effect of osmopriming on seed germination and early seedling characteristics of Carum carvi L. Agriculture, 10(4): 94.
  41. Murakami, K., Edamoto, M., Hata, N., Itami, Y. and Masuda, M. (2009). Low-oxalate spinach mutant induced by chemical mutagenesis. Journal of the Japanese Society for Horticultural Science, 78(2): 180-184.
  42. Omidi, A. H., Khazaei, H., Monneveux, P. and Stoddard, F. (2012). Effect of cultivar and water regime on yield and yield components in safflower (Carthamus tinctorius L.). Turkish Journal of Field Crops, 17(1): 10-15.
  43. Özer, U., Akın, B. and Altunok Memiş, A. (2024). The effect of different chemical mutagen treatments on in vitro seed germination and plant development in Sesame (Sesamum indicum L.). ANADOLU Journal of Aegean Agricultural Research Institute, 34(2): 196-202.
  44. Pillai, P. K. C., Viswanath, S. C., Hrideek, T. K. and Jiji, A. H. (2019). Seed Characteristics and Germination Behaviour of Bauhinia malabarica Roxb. In: Horticultural Crops, Ed(s): Baimey H. K, Hamamouch, N. and Kolombia, Y. A., IntechOpen, United Kingdom.
  45. Poehlman, J. M. and Sleper, A. (1995). Breeding Field Crops. Iowa State University Press, Iowa, U. S. A.
  46. Roychowdhury, R. and Tah, J. (2011). Germination behaviors in M2 generation of Dianthus after chemical mutagenesis. International Journal of Advanced Scientific and Technical Research, 1: 448–454.
  47. Sahin, S. and Mengec, H. O. (2022). Determination of the effects of some additives added to the mixture of diesel and safflower biodiesel on exhaust emissions. Journal of Tekirdag Agricultural Faculty, 19(4): 769-787.
  48. Sanghani, J. M., Vadher, K. J., Sanghani, A. O., Ramani, H. R., Raval, S. S. and Sorathiya, J. S. (2016). Effect of chemical mutagens on character association in sesame (Sesamum indicum L.). Research Journal of Biotechnology, 11(7): 11-18.
  49. Sari, N. K. Y., Pharmawati, M. B. and Junitha, I. K. (2012). Effects of sodium azide on morphological traits of Capsicum annuum L. Jurnal Metamorfosa, 1(1): 25-28.
  50. Schaberg, P., Murakami, P., Turner, M., Heitz, H. and Hawley, G. (2008). Association of red coloration with senescence of sugar maple leaves in autumn. Trees, 22: 573–578.
  51. Shams, M., Yildirim, E., Guleray, A., Ercisli, S., Dursun, A. et al. (2018). Nitric oxide alleviates copper toxicity in germinating seed and seedling growth of Lactuca sativa L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(1): 167-172.
  52. Sikder, S., Biswas, P., Hazra, P., Akhtar, S., Chattopadhyay, A. et al. (2013). Induction of mutation in tomato (Solanum lycopersicum L.) by gamma irradiation and EMS. Indian Journal of Genetics and Plant Breeding, 73(4): 392-399.
  53. Smith, J. R. (1996). Safflower. The American Oil Chemists Society Press, Champaign, U. S. A.
  54. Soufi, A. (2024). Effect of soaking seeds with graded concentrations of ethyl methane sulfonate (EMS) on local tobacco plants under salt stress conditions. Asian Journal of Advances in Research, 7(1): 289–296.
  55. Talukdar, D. (2011). Isolation and characterization of NaCl-tolerant mutations in two important legumes, Clitoria ternatea L. and Lathyrus sativus L.: Induced mutagenesis and selection by salt stress. Journal of Medicinal Plants Research, 5(16): 3619-3628.
  56. Tantray, A. Y., Raina, A., Khursheed, S., Amin, R. and Khan, S. (2017). Chemical mutagen affects pollination and locule formation in capsules of black cumin (Nigella sativa L.). International Journal of Agricultural Science, 8(1): 108-117.
  57. Taziun, T., Laskar, R. A., Amin, R. and Parveen, S. K. K. (2018). Effects of dosage and durations of different mutagenic treatment in lentil (Lens culinaris Medik.) cultivars Pant L 406 and DPL 62. Legume Research, 41(4): 500-509.
  58. Tripathi, S., Mewar, D., Prasad, B. and Jain, V. K. (2012). Standardization of temperature regimes for better seed germination and seedling growth of burdock (Arctium lappa). Journal of Hill Agriculture, 3(2): 77-81.
  59. Turanlı, H., Çoban, F. and Haliloğlu, K. (2025). Optimization of sodium azide application in black cumin (Nigella sativa L.) and determination of agronomic characteristics in M1 generation. Turkish Journal of Agricultural and Natural Sciences, 12(3): 753-763.
  60. Uskutoğlu, T. (2022). The effect of different chemical mutagen applications on some agricultural and technological properties of safflower (Carthamus tinctorius L.) seeds in the M1 and M2 generations. (PhD. Thesis) Kahramanmaras Sütcü Imam University, The Institute of Natural Sciences, Kahramanmaras, Türkiye.
  61. Vilatersana, R., Susana, A., Garcia-Jacas, N. and Garnatie, T. (2000). Generic delimitation and phylogeny of the Carduncellus–Carthamus complex (Asteraceae) based on ITS sequences. Plant Systematics and Evolution, 221: 189–105.
  62. Waghmare, V. N. and Mehra, R. B. (2001). Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrus sativus L. Indian Journal of Genetics and Plant Breeding, 61(1): 53-56.
  63. Wibowo, A., Nugroho, D. and Sumirat, U. (2020). Seed germination performance of nine Arabica coffee (Coffea arabica L.) varieties under the laboratory condition after six months of storage period. Pelita Perkebunan, 36(3): 203-211.
  64. Winkler, H. M., Cereijo, A. E., Scarpin, G. J., Dileo, P. N., Muchut, R. J., Roeschlin, R. A., Lorenzini, F. G., Paytas, M. J. and Landau, A. M. (2023). Phenotypic effects of different doses of physical and chemical mutagens in cotton plants. RIA. Revista de Investigaciones Agropecuarias, 49(2): 71-84.
  65. Yıldırım, M., Akıncı, C., Müjde, K. and Barutçular, C. (2009). Applicability of canopy temperature depression and chlorophyl content in durum wheat breeding. Anadolu Journal of Agricultural Sciences, 24(3): 158-166.
  66. Yüzbaşoğlu, E., Dalyan, E. and Akpınar, I. (2017). Changes in photosynthetic pigments, anthocyanin content and antioxidant enzyme activities of maize (Zea mays L.) seedlings under high temperature stress conditions. Trakya University Journal of Natural Sciences, 18(2): 97-1.
  67. Zhang, B. H. (2000). Regulation of plant growth regulators on cotton somatic embryogenesis and plant regeneration. Biochemistry, 39: 1567.