Effect of Enterococcus faecium on the Digestive Tract of Poultry as a Probiotic

Temel Bulgular & Veri:Microbiological characteristics were represented by CFU of Escherichia coli, faecal Enterococci and Lactobacilli determined in 1 g of chyme and biological ones by enzymatic activity of the cellulases expressed in CMC units.

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Sonuç & Katkı:Polymerase chain reaction using specific primers was performed to identify Enterococcus faecium.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Enterococci · enzymatic activity of cellulases · Escherichia coli

Özet

This project investigates the microbiological and biochemical characteristics of chicken and turkey caeca. Microbiological characteristics were represented by CFU of Escherichia coli, faecal Enterococci and Lactobacilli determined in 1 g of chyme and biological ones by enzymatic activity of the cellulases expressed in CMC units. In all experiments, a probiotic preparation based on Enterococcus faecium was added to the feeding mixture and drinking water at various concentrations or in the form of aerosol on the body or egg surface. Counts of Escherichia coli in CFU in 1 g of faecal chyme were determined on Endo agar, counts of CFU of faecal Enterococci on selective agar for faecal Enterococci and counts of Lactobacilli on Rogosa agar. Enzymatic activity of cellulases was determined according to the Miller method. Counts of CFU of Escherichia coli, faecal Enterococci, Lactobacilli and enzymatic activity of cellulases were compared in experimental and control treatments. Theoretical and empirical evidence suggests that the counts of Escherichia coli CFU would be higher in control treatments and CFU counts of both faecal Enterococci and Lactobacilli as well as enzymatic cellulases activity (in CMC units) lower in control treatments compared to the experimental ones. Similar results were also obtained in our experiments with chickens and turkeys. Polymerase chain reaction using specific primers was performed to identify Enterococcus faecium.

Abstract

This project investigates the microbiological and biochemical characteristics of chicken and turkey caeca. Microbiological characteristics were represented by CFU of Escherichia coli, faecal Enterococci and Lactobacilli determined in 1 g of chyme and biological ones by enzymatic activity of the cellulases expressed in CMC units. In all experiments, a probiotic preparation based on Enterococcus faecium was added to the feeding mixture and drinking water at various concentrations or in the form of aerosol on the body or egg surface. Counts of Escherichia coli in CFU in 1 g of faecal chyme were determined on Endo agar, counts of CFU of faecal Enterococci on selective agar for faecal Enterococci and counts of Lactobacilli on Rogosa agar. Enzymatic activity of cellulases was determined according to the Miller method. Counts of CFU of Escherichia coli, faecal Enterococci, Lactobacilli and enzymatic activity of cellulases were compared in experimental and control treatments. Theoretical and empirical evidence suggests that the counts of Escherichia coli CFU would be higher in control treatments and CFU counts of both faecal Enterococci and Lactobacilli as well as enzymatic cellulases activity (in CMC units) lower in control treatments compared to the experimental ones. Similar results were also obtained in our experiments with chickens and turkeys. Polymerase chain reaction using specific primers was performed to identify Enterococcus faecium.

Anahtar kelimeler

Enterococci enzymatic activity of cellulases Escherichia coli Lactobacilli probiotic

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Bu Makaleye Atıf Yap

KACÁNIOVÁ, M., KMET, V., CUBON, J. (2006). Effect of Enterococcus faecium on the Digestive Tract of Poultry as a Probiotic. Turkish Journal of Veterinary and Animal Sciences, Cilt: 30 - Sayı: 3, 291–298. https://ajindex.com/articles/132347

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Kaynakça (12)

Lee, Y.K., Salminen, S.: The coming of age of probiotics. Trends Food Sci. Technol., 1995; 6: 241-245.
Burgstaller, G., Ferstl, R., Alps, H.: Zum Zuchsatz von Milchsäurebakterien (Streptococcus faecium SF - 68) in Milchaustauschfuttermittel fur Mastkälber. Züchtungskunde, 1984; 56: 156-162. 3. Wallace, R.J., Newbold, C.J.: Probiotics for ruminants. In: Fuller, R.: Probiotics: the Scientific Basis. Chapman and Hall, London. 1992; 317-353. Angelovi˘cová, M., Michalik, I.: A test of enzymatic preparation in relation to performance and commercial utilization of feeds in broiler chickens. Zivocisna Vyroba, 1997; 42: 175-180.
Kivanc, M.: Antagonistic lactic cultures toward spoilage and pathogenic microorganisms in food. Nahrung, 1990; 34: 273- 277. 6. Watkins, B.A., Miller, B.F., Neil, D.H.: In vivo inhibitory effects of Lactobacillus acidophilus against pathogenic Escherichia coli in gnotobiotic chicks. Poult. Sci., 1982; 61: 1298-1308. 7. Gibson, G.R., Beatty, E.R., Wang, X., Cummings, J.H.: Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology, 1995; 108: 975-982.
Christl, S.U., Gibson, G.R., Cummings, J.H.: Role of dietary sulphate in the regulation of methanogenesis in the human large intestine. Gut, 1992; 33: 1234-1238.
Holt, G.J., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T.: Bergey's Manual of Determinative Bacteriology. Baltimore, Williams and Wilkins. 1994; 787. Miller, G.L., Blum, R., Glennon, W.E., Buton, A.L.: Measurement of carboxymethylcellulase activity. Anal. Biochem., 1960; 23: 257-270.
Dutka-Malen, S., Evers, S., Courvalin, P.: Detection of glycopepetide resistance genotypes and identification to the species level of clinically relevant eneterococci by PCR. J. Clin. Microbiol., 1995; 33: 24-28.
Tannock, G.W.: Probiotics and prebiotics: Scientific aspects. Caister Academic Press,
Wymondham, UK, 2005, 230. Qureshi, M.A., Ali, R., Cheema, M.A., Ahmed, Z., Roth, H.: Immunmilk feeding increases growth and immune responses in broiler chicks. Int. J. Poult. Sci., 2004; 3: 305-312.
Fuller, R.: Probiotics 2: applications and practical aspects. Chapman and Hall, London UK, 1-209.
Tannock, G.W.: Probiotics and prebiotics: where are we going? Caister Academic Press,
Wymondham, UK. 2002, 336. Ka˘cániová, M.: The effect of probiotics to microbiological and biochemical indexes in the ceacum of chickens and turkeys. PhD Dissertation. Slovak University of Agriculture in Nitra, SK. 2001.
Nava, G.M.