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Dergiler / Online Türk Sağlık Bilimleri Dergisi / 2026 / Cilt: 8 Sayı: 1

Sisplatin ve Karboplatin Yüklü Albumin Nanopartiküllerin Meme Kanseri Hücreleri Üzerindeki Sitotoksik Etkileri

Cytotoxic Effects of Cisplatin and Carboplatin Loaded Albumin Nanoparticles on Breast Cancer Cells

Ferdane Danışman Kalındemirtaş(Erzincan Üniversitesi Tıp Fakültesi)0000-0001-7085-8596
İshak Afşin Kariper(ERCİYES ÜNİVERSİTESİ)0000-0001-9127-301X
Sayfa
115–121
Yayın Tarihi
2023-03-05
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: CP-NPs’lerin boyutu ortalama 2-3 nm civarında iken, Cis-NPs’lerin 7-8 nm idi.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Bu çalışmanın amacı kanser tedavisinde sıklıkla kullanılan karboplatin ve sisplatin ilaçlarının albümin nanotaşıyıcıya yüklenerek antikanser etkilerinin araştırılması ve karşılaştırılmasıdır.
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Temel Bulgular & Veri:Nanopartikül boyutu ve dağılımın homojenitesi Dinamik ışık saçılımı (DLS) ile değerlendirildi.
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Sonuç & Katkı:Bulgular: CP-NPs’lerin boyutu ortalama 2-3 nm civarında iken, Cis-NPs’lerin 7-8 nm idi.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:MCF-7 · MDA-MB-231 · meme kanseri

Özet

Amaç: Bu çalışmanın amacı kanser tedavisinde sıklıkla kullanılan karboplatin ve sisplatin ilaçlarının albümin nanotaşıyıcıya yüklenerek antikanser etkilerinin araştırılması ve karşılaştırılmasıdır. Materyal ve Metot: Karboplatin (CP) ve Sisplatin (Cis) yüklenmiş albümin nanopartiküller, sırasıyla CP-NPs ve Cis-NPs olarak ultrasonikasyon kullanarak sentezlendi. Nanopartikül boyutu ve dağılımın homojenitesi Dinamik ışık saçılımı (DLS) ile değerlendirildi. Nanopartiküllerin sitotoksik aktiviteleri MDA-MB-231 ve MCF-7 meme kanseri hücrelerinde ve HUVEC hücrelerinde, MTT testi kullanılarak değerlendirildi ve morfolojik görüntüleri karşılaştırıldı. Bulgular: CP-NPs’lerin boyutu ortalama 2-3 nm civarında iken, Cis-NPs’lerin 7-8 nm idi. Her iki nanopartikül grubunun da homojen bir şekilde dağıldığı görüldü. Sitotoksisite sonuçlarına göre CP-NPs ve Cis-NPs, MCF-7 meme kanseri hücrelerinde daha sitotoksikti. Ayrıca CP-NPs ve Cis-NPs’ler MDA-MB-231 meme kanseri hücrelerinde önemli sitotoksisite gösterirken, normal HUVEC hücrelerinde düşük sitotoksisite tespit edildi. CP-NPs ve Cis-NPs ile tedavi edilen MCF-7, tedavi edilmeyen MCF-7 ile karşılaştırıldı ve NPs'ler için istatistiksel anlamlılık P<0,01 olarak hesaplandı. Sonuç: Meme kanseri hücrelerinde yüksek sitotoksisite gözlenirken, sağlıklı hücrelerde belirgin sitotoksisite gözlenmemiş olup albümine bağlı CP-NPs ve Cis-NPs kanser tedavisinde umut vadedici bir tedavi seçeneği olabilir.

Abstract

Objective: This study aims to investigate and compare the anticancer effects of carboplatin and cisplatin, frequently used in cancer treatment, by loading them on albumin nanocarrier. Materials and Methods: Carboplatin (CP) and Cisplatin (Cis) loaded albumin nanoparticles were synthesized using ultrasonication as CP-NPs and Cis-NPs, respectively. Nanoparticle size and distribution were evaluated by Dynamic light scattering (DLS). Cytotoxicities of NPs were evaluated in MDA-MB-231 and MCF-7 breast cancer cells and HUVEC using MTT test and their morphological images were compared. Results: While the average size of CP-NPs was around 2-3 nm, Cis-NPs was 7-8 nm. It was observed that both NPs groups were homogeneously dispersed. According to the cytotoxicity results, both CP-NPs and Cis-NPs were more cytotoxic on MCF-7 breast cancer cells. In addition, CP-NPs and Cis-NPs showed significant cytotoxicity on MCF-7, MDA-MB-231 breast cancer cells, while low cytotoxicity was detected in normal HUVEC cells. The NPs treated MCF-7 was compared with the untreated MCF-7 and statistical significance was calculated as P<0.01 for CP-NPs and Cis-NPs. Conclusions: Abumin-based CP-NPs and Cis-NPs showed high cytotoxicity in breast cancer cells, they have low cytotoxicity in healthy cells, making them promising for breast cancer treatment.

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Bu Makaleye Atıf Yap

Kalındemirtaş, F. D., Kariper, İ. A. (2023). Sisplatin ve Karboplatin Yüklü Albumin Nanopartiküllerin Meme Kanseri Hücreleri Üzerindeki Sitotoksik Etkileri. Online Türk Sağlık Bilimleri Dergisi, Cilt: 8 Sayı: 1, 115–121. https://doi.org/10.26453/otjhs.1217364

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