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Dergiler / Sakarya Tıp Dergisi / 2026 / Volume: 16 Issue: 1

Rosmarinic Acid Mitigates Oxidative–Inflammatory Injury in Rat Testicular Torsion–Detorsion Model

Mustafa Can Güler(ATATURK UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0000-0001-8588-1035
Hüseyin Baylan(SAKARYA UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF ANATOMY)0000-0002-9150-9210
Derya Güzel Erdoğan(SAKARYA UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0000-0002-7618-5043
Ayhan Tanyeli(ATATURK UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF PHYSIOLOGY)0000-0002-0095-0917
Ersen Eraslan(BANDIRMA ONYEDI EYLUL UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL PHYSIOLOGY)0000-0003-2424-2269
Fazile Nur Ekinci Akdemir(UNIVERSITY OF HEALTH SCIENCES, ERZURUM MEDICINE FACULTY, DEPARTMENT OF PHYSIOLOGY)0000-0001-9585-3169
Sayfa
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Yayın Tarihi
2026-03-28
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Conclusions: RA attenuates oxidative and inflammatory injury in testicular T/D as a peri-detorsion adjunct.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Objective: We aimed to determine whether pre-detorsion rosmarinic acid (RA) mitigates oxidative and inflammatory damage in a rat torsion–detorsion (T/D)-induced I/R injury model.
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Temel Bulgular & Veri:Methods: Thirty-two male rats were randomised (n=8/group) to sham, T/D, T/D + RA 40 mg/kg, or T/D + RA 80 mg/kg.
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Sonuç & Katkı:Conclusions: RA attenuates oxidative and inflammatory injury in testicular T/D as a peri-detorsion adjunct.
🔬Tür:Modelleme & Sayısal Simülasyon
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Testicular torsion-detorsion · Ischemia-reperfusion · Rosmarinic acid

Abstract

Objective: We aimed to determine whether pre-detorsion rosmarinic acid (RA) mitigates oxidative and inflammatory damage in a rat torsion–detorsion (T/D)-induced I/R injury model. Methods: Thirty-two male rats were randomised (n=8/group) to sham, T/D, T/D + RA 40 mg/kg, or T/D + RA 80 mg/kg. T/D consisted of 2 h torsion followed by 2 h reperfusion. RA (i.p.) was administered 30 min before detorsion. Testis tissue was analysed for oxidative stress markers (MDA, MPO, OSI, TOS), antioxidant parameters (SOD, TAS), and inflammatory cytokines (TNF-α, IL-1β). Results: Compared with sham, T/D caused high MDA, MPO, TNF-α, TOS/OSI, IL-1β, and low SOD, TAS. RA significantly reversed these changes versus T/D, lowering oxidative and inflammatory markers and enhancing antioxidant defences. The high dose RA was equal or superior to low dose—with clearer gains for SOD, TAS, MPO, and cytokines—whereas TOS/OSI improvements were similar at both doses. Conclusions: RA attenuates oxidative and inflammatory injury in testicular T/D as a peri-detorsion adjunct.

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Bu Makaleye Atıf Yap

Güler, M. C., Baylan, H., Erdoğan, D. G., Tanyeli, A., Eraslan, E., Akdemir, F. N. E. (2026). Rosmarinic Acid Mitigates Oxidative–Inflammatory Injury in Rat Testicular Torsion–Detorsion Model. Sakarya Tıp Dergisi, Volume: 16 Issue: 1. https://doi.org/10.31832/smj.1776904

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Kaynakça (41)

  1. Shahedi A, Talebi AR, Mirjalili A, Pourentezari M. Protective effects of curcumin on chromatin quality, sperm parameters, and apoptosis following testicular torsion-detorsion in mice. Clin Exp Reprod Med. 2021;48(1):27. doi:10.5653/cerm.2020.03853.
  2. Demirkapu MJ, Karabag S, Akgul HM, Mordeniz C, Yananli HR. The effects of etomidate on testicular ischemia reperfusion injury in ipsilateral and contralateral testes of rats. Eur Rev Med Pharmacol Sci. 2022;26(1):211-17. doi:10.26355/eurrev_202201_27770.
  3. Davoodi F, Taheri S, Raisi A, Rajabzadeh A, Ahmadvand H, Hablolvarid MH, et al. Investigating the sperm parameters, oxidative stress and histopathological effects of Salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats. Theriogenology. 2020;144:98-106. doi:10.1016/j.theriogenology.2020.01.002.
  4. Polat Kose L, Gulcin İ. Evaluation of the antioxidant and antiradical properties of some phyto and mammalian lignans. Molecules. 2021;26(23):7099. doi:10.3390/molecules26237099.
  5. Durmaz L, Erturk A, Akyüz M, Polat Köse L, Uc EM, Bingol Z, et al. Screening of carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α-glycosidase enzyme inhibition effects and antioxidant activity of coumestrol. Molecules. 2022;27(10):3091. doi:10.3390/molecules27103091.
  6. Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, et al. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Arch Toxicol. 2023;97(10):2499-574. doi:10.1007/s00204-023-03562-9.
  7. Yapıcı İ, Altay A, Öztürk Sarıkaya B, Korkmaz M, Atilla A, Gülçin İ, et al. In vitro antioxidant and cytotoxic activities of extracts of endemic Tanacetum erzincanense together with phenolic content by LC-ESI-QTOF-MS. Chem Biodivers. 2021;18(3):e2000812. doi:10.1002/cbdv.202000812.
  8. Chandimali N, Bak SG, Park EH, Lim HJ, Won YS, Kim EK, et al. Free radicals and their impact on health and antioxidant defenses: a review. Cell Death Discov. 2025;11(1):19. doi:10.1038/s41420-024-02278-8.
  9. Güler MC, Tanyeli A, Ekinci Akdemir FN, Eraslan E, Özbek Şebin S, Güzel Erdoğan D, et al. An overview of ischemia-reperfusion injury: review on oxidative stress and inflammatory response. Eurasian J Med. 2022;54(Suppl 1):S62. doi:10.5152/eurasianjmed.2022.22293.
  10. Guan H, Luo W, Bao B, Cao Y, Cheng F, Yu S, et al. A comprehensive review of rosmarinic acid: from phytochemistry to pharmacology and its new insight. Molecules. 2022;27(10):3292. doi:10.3390/molecules27103292.
  11. Singh D, Kumari K, Ahmed S. Natural herbal products for cancer therapy. In: Understanding Cancer. Academic Press; 2022. p. 257-68. doi:10.1016/B978-0-323-99883-3.00010-X.
  12. Elufioye TO, Habtemariam S. Hepatoprotective effects of rosmarinic acid: insight into its mechanisms of action. Biomed Pharmacother. 2019;112:108600. doi:10.1016/j.biopha.2019.108600.
  13. Topal M, Gulcin İ. Evaluation of the in vitro antioxidant, antidiabetic and anticholinergic properties of rosmarinic acid from rosemary (Rosmarinus officinalis L.). Biocatal Agric Biotechnol. 2022;43:102417. doi:10.1016/j.bcab.2022.102417.
  14. Zhang T, Liu C, Ma S, Gao Y, Wang R. Protective effect and mechanism of action of rosmarinic acid on radiation-induced parotid gland injury in rats. Dose Response. 2020;18(1):1559325820907782. doi:10.1177/1559325820907782.
  15. Kowalczyk A, Tuberoso CIG, Jerković I. The role of rosmarinic acid in cancer prevention and therapy: mechanisms of antioxidant and anticancer activity. Antioxidants. 2024;13(11):1313. doi:10.3390/antiox13111313.
  16. Oğuz A, Böyük A, Ekinci A, Alabalik U, Türkoğlu A, Tuncer MC, et al. Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: a biochemical and histopathological study. Folia Morphol (Warsz). 2020;79(2):288-95. doi:10.5603/FM.a2019.0087.
  17. Jiang K, Ma X, Guo S, Zhang T, Zhao G, Wu H, et al. Anti-inflammatory effects of rosmarinic acid in lipopolysaccharide-induced mastitis in mice. Inflammation. 2018;41(2):437-48. doi:10.1007/s10753-017-0700-8.
  18. Noor S, Mohammad T, Rub MA, Raza A, Azum N, Yadav DK, et al. Biomedical features and therapeutic potential of rosmarinic acid. Arch Pharm Res. 2022;45(4):205-28. doi:10.1007/s12272-022-01378-2.
  19. Han J, Wang D, Ye L, Li P, Hao W, Chen X, et al. Rosmarinic acid protects against inflammation and cardiomyocyte apoptosis during myocardial ischemia/reperfusion injury by activating PPAR-γ. Front Pharmacol. 2017;8:456. doi:10.3389/fphar.2017.00456.
  20. Taheri S, Davoodi F, Raisi A, Zakian A, Rajabzadeh A, Hablolvarid MH, et al. Co-administration of Salvia miltiorrhiza and verapamil inhibits detrimental effects of torsion/detorsion on testicular tissue in rats. Andrologia. 2021;53(6):e14049. doi:10.1111/and.14049.
  21. Raisi A, Davoodi F, Mohammadi R. Protective effects of rosmarinic acid on testicular torsion-detorsion in an animal model. Iran J Vet Surg. 2022;17(2):80-6. doi:10.30500/ivsa.2022.327175.1293.
  22. Asl JF, Goudarzi M, Mansouri E, Shoghi H. Rosmarinic acid protects the testes of rats against cell phone and ultra-high frequency waves induced toxicity. Iran J Med Sci. 2024;49(4):237. doi:10.30476/ijms.2023.97695.2952.
  23. Ekinci Akdemir FN, Güler MC, Eraslan E, Tanyeli A, Yildirim S. Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury. Sci Rep. 2024;14(1):31385. doi:10.1038/s41598-024-82912-8.
  24. Tanyeli A, Güler MC, Eraslan E, Özbek Şebin S, Bircan B, Şebin E, et al. Potential beneficial effects of apelin-13 on testicular ischemia/reperfusion injury. New Trends Med Sci. 2024;5(2):98-103. doi:10.56766/ntms.1476451.
  25. Öztürk D, Tanyeli A, Güzel D, Güler MC, Eraslan E, Baylan H. The effects of myricetin against testicular and lung injury induced by testicular ischemia reperfusion model. Sakarya Med J. 2021;11(1):109-14. doi:10.31832/smj.783480.
  26. Tanyeli A, Eraslan E, Güler MC, Ekinci Akdemir FN, Güzel Akdoğan D, Topdağı Ö, et al. Investigation of the effects of maresin-1 on testicular ischemia reperfusion induced oxidative stress. South Clin Ist Euras. 2020;31(3):187. doi:10.14744/scie.2020.39205.
  27. Tanyeli A, Eraslan E, Ekinci Akdemir FN, Can Güler M, Güzel Erdoğan D, Popalt E, et al. Cryptotanshinone mitigates ischemia reperfusion-induced testicular damage: an experimental study. Ann Med Res. 2019;26(11):2549-2552. doi:10.5455/annalsmedres.2019.09.535.
  28. Hassanzadeh-Taheri M, Ahmadi-Zohan A, Mohammadifard M, Hosseini M. Rosmarinic acid attenuates lipopolysaccharide-induced neuroinflammation and cognitive impairment in rats. J Chem Neuroanat. 2021;117:102008. doi:10.1016/j.jchemneu.2021.102008.
  29. Akdemir FNE, Güler MC, Eraslan E, Tanyeli A, Yildirim S. Assessment of sinapic acid’s protective effects against ethanol-induced gastric ulcers in rats. Naunyn Schmiedebergs Arch Pharmacol. 2025;398(6):7133-45. doi:10.1007/s00210-024-03733-0.
  30. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8. doi:10.1016/0003-2697(79)90738-3.
  31. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982;78(3):206-9. doi:10.1111/1523-1747.ep12506462.
  32. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500. doi:10.1093/clinchem/34.3.497.
  33. Laher A, Ragavan S, Mehta P, Adam A. Testicular torsion in the emergency room: a review of detection and management strategies. Open Access Emerg Med. 2020;12:237-46. doi:10.2147/OAEM.S236767.
  34. Hu S, Guo M, Xiao Y, Li Y, Luo Q, Li Z, et al. Mapping trends and hotspot regarding testicular torsion: a bibliometric analysis of global research (2000–2022). Front Pediatr. 2023;11:1121677. doi:10.3389/fped.2023.1121677.
  35. Jafari A, Ghasemnejad-Berenji H, Nemati M, Pashapour S, Sadeghpour S, Ghasemnejad-Berenji M, et al. Beneficial effects of memantine on ischemia/reperfusion injury following torsion/detorsion induced testicular damage in rats: improvement in histological and biochemical parameters. J Pediatr Urol. 2021;17(4):441.e1-441.e8. doi:10.1016/j.jpurol.2021.04.014.
  36. Şentürk İY, Değer MD, Aydın MA, Madendere S, Kaya O, Taştekin E, et al. Pioglitazone eases testicular torsion/detorsion-induced ischemia-reperfusion injury in rats. J Urol Surg. 2023;10(2):167-72. doi:10.4274/jus.galenos.2022.2022.0047.
  37. Arena S, Iacona R, Antonuccio P, Russo T, Salvo V, Gitto E, et al. Medical perspective in testicular ischemia-reperfusion injury. Exp Ther Med. 2017;13(5):2115-22. doi:10.3892/etm.2017.4289.
  38. Koptielow J, Szyłak E, Koptielowa A, Sarnowska M, Kapica-Topczewska K, Adamska-Patruno E, et al. Dystonia versus redox balance: a preliminary assessment of oxidative stress in patients. Antioxidants. 2025;14(9):1052. doi:10.3390/antiox14091052.
  39. Jomova K, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, Valko M. Several lines of antioxidant defense against oxidative stress: antioxidant enzymes, nanomaterials with multiple enzyme-mimicking activities, and low-molecular-weight antioxidants. Arch Toxicol. 2024;98(5):1323-67. doi:10.1007/s00204-024-03696-4.
  40. Lin W, Chen H, Chen X, Guo C. The roles of neutrophil-derived myeloperoxidase (MPO) in diseases: the new progress. Antioxidants. 2024;13(1):132. doi:10.3390/antiox13010132.
  41. Ramalho LNZ, Pasta ÂAC, Terra VA, Augusto M, Sanches SC, Souza-Neto FP, et al. Rosmarinic acid attenuates hepatic ischemia and reperfusion injury in rats. Food Chem Toxicol. 2014;74:270-8. doi:10.1016/j.fct.2014.10.004.