KaydedilenlerDerginizi ekleyin

Dergiler / Uludağ Arıcılık Dergisi / 2026 / Volume: 26

Comparative in vitro antibacterial activities of chestnut and flower honeys against selected pathogenic bacteria

Ayşe Ebru Borum(BALIKESIR UNIVERSITY, FACULTY OF VETERINARY, VETERINARIAN PR.)0000-0002-6916-8982
Mustafa Keles(BALIKESİR ÜNİVERSİTESİ)0009-0005-1501-4549
Arda Güreşmen(BALIKESİR ÜNİVERSİTESİ)0009-0004-7978-8458
Fatih Feyyaz Alagöz(BALIKESİR ÜNİVERSİTESİ)0009-0001-7949-2161
Murat Vicdan(BALIKESİR ÜNİVERSİTESİ)0009-0002-8715-0978
Sayfa
—
Yayın Tarihi
2026-07-01
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The conclusion drawn from this research is that the floral origin of honey and applied concentration are decisive factors in antibacterial activity, and that chestnut honey has significant potential as a natural antibacterial agent.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The antibacterial properties of natural products have attracted increasing attention in recent years amid rising antimicrobial resistance.
📊
Temel Bulgular & Veri:The honeys were tested at 100% and 25% concentrations.
💡
Sonuç & Katkı:The conclusion drawn from this research is that the floral origin of honey and applied concentration are decisive factors in antibacterial activity, and that chestnut honey has significant potential as a natural antibacterial agent.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Antibacterial activity · Chestnut honey · Flower honey

Abstract

The antibacterial properties of natural products have attracted increasing attention in recent years amid rising antimicrobial resistance. The present study evaluated the antibacterial activity of chestnut and flower honeys, sourced from various provinces of Türkiye. The honeys were tested at 100% and 25% concentrations. The standard strains utilised in this study encompassed Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 43894), Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 43300), and Salmonella enterica serovar Enteritidis (ATCC 13076). The antibacterial activity was determined using the agar diffusion method, and the inhibition zones were evaluated. It was observed that the antibacterial activity of flower honeys varied with strain and concentration, with a significant decrease at low concentrations. In contrast, chestnut honey exhibited a stronger, more consistent antibacterial effect against all tested bacterial strains, particularly at 100% concentration. The conclusion drawn from this research is that the floral origin of honey and applied concentration are decisive factors in antibacterial activity, and that chestnut honey has significant potential as a natural antibacterial agent.

🔖

Bu Makaleye Atıf Yap

Borum, A. E., Keles, M., Güreşmen, A., Alagöz, F. F., Vicdan, M. (2026). Comparative in vitro antibacterial activities of chestnut and flower honeys against selected pathogenic bacteria. Uludağ Arıcılık Dergisi, Volume: 26. https://doi.org/10.31467/uluaricilik.1931153

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (42)

  1. Albaridi NA. Antibacterial Potency of Honey. International Journal of Microbiology 2019;2019:2464507. https://doi.org/10.1155/ 2019/2464507
  2. Almasaudi SB, Abbas AT, Al–Hindi RR, El–Shitany NA, Abdel–Dayem UA, Ali SS, et al. Manuka honey exerts antioxidant and anti–inflammatory activities that promote healing of acetic acid–induced gastric ulcer in rats. Evidence–Based Complementary and Alternative Medicine 2017;2017:5413917. https://doi.org/10.1155/2017/5413917
  3. Al–Kafaween MA, Alwahsh M, Mohd Hilmi AB, Abulebdah DH. Physicochemical Characteristics and Bioactive Compounds of Different Types of Honey and Their Biological and Therapeutic Properties: A Comprehensive Review. Antibiotics (Basel). 2023;12(2):337.https://doi:10.3390/antibiotics 12020337
  4. Almasaudi S. The antibacterial activities of honey. Saudi Journal of Biological Sciences 2021;28:2188–2196. https://doi.org/10.1016/ j.sjbs.2020.10.017.
  5. Alshaybawee MR, Asgari S, Ghadersoltani P, Mehrabian A, Saniee P. Exploring the antibacterial and anti–biofilm activity of two Iranian medical–grade kinds of honey on multidrug–resistant Pseudomonas aeruginosa. BMC Complementary Medicine and Therapies 2025;25:39. https://doi.org/ 10.1186/s12906-025-04778-1
  6. Ani S, Ika R, Patel A, Gupta VK. Pharmacological and antioxidant evaluation of natural honey samples. International Journal of Biotech Trends and Technology 2020;10:31–38. https://doi.org/10.14445/22490183/
  7. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis 2016;6(2):71–79. https://doi:10.1016/j.jpha. 2015.11.005
  8. Bergman A, Yanai J, Weiss J, Bell D, David MP. Acceleration of wound healing by topical application of honey: an animal model. The American Journal of Surgery 1983; 145:374–376. https://doi.org/10.1016/0002-10(83) 90204-0
  9. Borum AE. Kestane balının antibakteriyel ve antifungal etkinliğinin incelenmesi. Uludag Bee Journal 2016;15:60–66. https://doi.org/ 10.31467/uluaricilik.377024
  10. Burlando B, Cornara L. Honey in dermatology and skin care: a review. Journal of Cosmetic Dermatology 2013;12:306–313.
  11. Carter DA, Blair SE, Cokcetin NN, Bouzo D, Brooks P, Schothauer R, et al. Therapeutic manuka honey: no longer so alternative. Frontiers in Microbiology 2016;7:569. https://doi.org/10. 3389/fmicb.2016.00569
  12. Čengić B, Rondić M, Jerković–Mujkić A, Šarić–Medić B, Magoda A, Ćutuk A, et al. Antimicrobial effectiveness of chestnut honey, pollen and propolis individually and in combination. Veterinarska Stanica 2025;56:203–214. https://doi.org/10.46419/ vs.56.2.3
  13. Cornara L, Biagi M, Xiao J, Burlando B. Therapeutic properties of bioactive compounds from different honeybee products. Frontiers in Pharmacology 2017;8:261216. https://doi. org/10.3389/fphar.2017.00412
  14. Eteraf–Oskouei T, Najafi M. Traditional and modern uses of natural honey in human diseases: a review. Iran Journal of Basic Medical Sciences 2013;16:731–742.
  15. El-kherbawy G, Hassan M, Abd Alla A. Herbal-infused Egyptian bee honey, a boon or a curse, its impact on sensorial, physicochemical & antibacterial properties. Uludag Bee Journal, 2024;24(2): 337-355.
  16. Fratianni F, Amato G, d’Acierno A, Ombra MN, De Feo V, Coppola R, et al. In vitro prospective healthy and nutritional benefits of different Citrus monofloral honeys. Scientific Reports 2023;13:1088. https://doi.org/10.1038/ s41598-023-27802-1
  17. Grabek–Lejko D, Worek M. Honeydew honey as a reservoir of bacteria with antibacterial and probiotic properties. Antibiotics 2024;13:855. https://doi.org/10.3390/antibiotics13090855.
  18. Güneş ME. Chestnut honey as a complementary medicine: determination of antibacterial activity, heavy metal residue and health risk assessment. Journal of Advances in VetBio Science and Techniques 2021;6:82–89. https://doi.org/10.31797/vetbio.931144.
  19. Hossain ML, Lim LY, Hammer K, Hettiarachchi D, Locher C. A review of commonly used methodologies for assessing the antibacterial activity of honey and honey products. Antibiotics 2022;11(7):975. https://doi.org/10. 3390/antibiotics11070975
  20. Israili ZH. Antimicrobial properties of honey. American Journal of Therapeutics 2014;21:304–323. https://doi.org/10.1097/ MJT.0b013e318293b09b.
  21. Jaganathan SK, Mandal SM, Jana SK, Das S, Mandal M. Studies on the phenolic profiling, anti–oxidant and cytotoxic activity of Indian honey: in vitro evaluation. Natural Product Research 2010;24:1295–1306. https://doi. org/10.1080/14786410903184408.
  22. Kačániová M, Borotová P, Galovičová L, Kunová S, Štefániková J, Kowalczewski PŁ, Šedík P. Antimicrobial and antioxidant activity of different Honey samples from beekeepers and Commercial Producers. Antibiotics 2022;11:1163. https://doi.org/10.3390/ antibiotics11091163.
  23. Kalaba V, Bojan G, Tanja I. Antibacterial activity of different types of honey on pathogenic bacteria. IX International Symposium on Agricultural Sciences AgroRe S 2020 Proceedings.
  24. Kamel AA, Marzouk WM, Hashish ME, Abd El Dayem MR. Synergistic antioxidant activity of honey bee products and their mixtures. Plant Archieves 2023;23:81–89.
  25. Kumera B, Gebreamanule B, Atnkut B, Merid M, Tilahun T, Tsega A, et al. In Vitro Antibacterial Activity of Raw Honey From Apis mellifera, Awi Zone, Amhara, Ethiopia. Microbiology Open 2025;14:e70076. https://doi.org/10. 1002/mbo3.70076.
  26. Molan P, Rhodes T. Honey: A Biologic Wound Dressing. Wounds: A Compendium of Clinical Research and Practice 2015;27:141–151.
  27. Meuller H. Antibacterial effect of honey against Escherichia coli, Salmonella Enteritidis and Listeria monocytogenes. Doctoral dissertation 2014.
  28. Ogbaje EO, Ogansanya T, Aiwerioba OIR. Conventional use of honey as antibacterial agent. Annals of African Medicine 2006;5:78–81.
  29. Ogwu MC, Izah SC. Honey as a natural antimicrobial. Antibiotics 2025;14:255. https://doi.org/10.3390/antibiotics14030255.
  30. Oryan A, Alemzadeh E, Mohammadi AA. Application of honey as a protective material in maintaining the viability of adipose stem cells in burn wound healing: A histological, molecular and biochemical study. Tissue and Cell 2019;61:89–97. https://doi.org/10.1016/ j.tice.2019.09.007.
  31. Petrović N, Lučić M, Radovanović B, Martić N. Comparing antipseudomonal effects of honey samples with different geographical and botanical origin. Global Pediatrics 2024;7:100149.
  32. Sagdic O, Silici S, Ekici L. Evaluation of the Phenolic Content, Antiradical, Antioxidant, and Antimicrobial Activity of Different Floral Sources of Honey.” International Journal of Food Properties 2013;16(3):658–66. doi:10.1080/10942912.2011.561463.
  33. Schneider M, Coyle S, Warnock M, Gow I, Fyfe L. Anti–Microbial Activity and Composition of Manuka and Portobello Honey. Phytotherapy Research 2013;27:1162–1168. https://doi. org/10.1002/ptr.4844.
  34. Swellam T, Miyanaga N, Onozawa M, Hattori K, Kawai K, Shimazui T, et al. Antineoplastic activity of honey in an experimental bladder cancer implantation model: in vivo and in vitro studies. International Journal of Urology 2003;10:213–219. https://doi.org/10.1046/ j.0919-172.2003.00602.x.
  35. Tan HT, Rahman RA, Gan SH, Halim AS, Hassan SA, Sulaiman SA, et al. The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complementary and Alternative Medicine 2009; 9:34, https://doi.org/10.1186/1472-6882-9-34.
  36. Tonks AJ, Cooper RA, Jones KP, Blair S, Parton J, Tonks A. Honey stimulates inflammatory cytokine production from monocytes. Cytokine. 2003;21(5):242-247, https://doi:10. 1016/s1043-4666(03)00092-9
  37. Vică ML, Glevitzky M, Dumitrel GA, Popa M, Glevitzky I, Teodoru CA. Antimicrobial Activity of Honey and Propolis from Alba County, Romania. Antibiotics 2024;13:952. doi:10.3390/antibiotics13100952.
  38. Wadi MA. In vitro antibacterial activity of different honey samples against clinical isolates. BioMed Research International 2022; 2022:1560050. https://doi:10.1155/2022/ 1560050
  39. Wilkinson JM, Cavanagh HM. Antibacterial activity of 13 honeys against Escherichia coli and Pseudomonas aeruginosa. Journal of Medicinal Food 2005;8:100–103. https://doi:10.1089/jmf.2005.8.100
  40. Grabek–Lejko D, Worek M. Honeydew honey as a reservoir of bacteria with antibacterial and probiotic properties. Antibiotics 2024;13:855. https://doi.org/10.3390/antibiotics13090855.
  41. Jaganathan SK, Mandal SM, Jana SK, Das S, Mandal M. Studies on the phenolic profiling, anti–oxidant and cytotoxic activity of Indian honey: in vitro evaluation. Natural Product Research 2010;24:1295–1306. https://doi. org/10.1080/14786410903184408.
  42. Oryan A, Alemzadeh E, Mohammadi AA. Application of honey as a protective material in maintaining the viability of adipose stem cells in burn wound healing: A histological, molecular and biochemical study. Tissue and Cell 2019;61:89–97. https://doi.org/10.1016/ j.tice.2019.09.007.