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Dergiler / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 49 Issue: 1

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Esin Akbay(Department of Biology, Hacettepe University)0000-0002-0797-8322
Y. Doruk Aracagök(Department of Biology, Hacettepe University)
Ali Hakan Göker(Central Instrumental Analysis II Laboratory, Ankara University)0000-0002-9366-6949
Mehmet Ali Onur(Department of Biology, Hacettepe University)0000-0002-3630-7982
Nilüfer Cihangir(Department of Biology, Hacettepe University)0000-0002-0830-635X
Sayfa
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Yayın Tarihi
2021-01-01
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen.
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Temel Bulgular & Veri:A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours.
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Sonuç & Katkı:Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Cytotoxicity · naproxen · 6-O-desmethylnaproxen

Abstract

The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by MTT, while apoptosis was determined by AO/PI double staining method. The control group was free from any agent (DMEM only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.

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Bu Makaleye Atıf Yap

Akbay, E., Aracagök, Y. D., Göker, A. H., Onur, M. A., Cihangir, N. (2021). In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry, Volume: 49 Issue: 1. https://doi.org/10.15671/hjbc.680564

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