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Dergiler / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 53 Issue: 3

Label-free Electrochemical Immunosensor for Ultrasensitive Detection of KLK4 Using Conjugated Polymer Modified Sensing Platform

Elif Burcu Aydın(TEKIRDAG NAMIK KEMAL UNIVERSITY)0000-0002-1982-4128
Sayfa
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Yayın Tarihi
2025-07-01
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The applicability of the immunosensor was tested in human serum samples and satisfactory results were obtained.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:In the present research, a label-free impedimetric kallikrein 4 (KLK4) biosensor was fabricated by using a low-cost and disposable indium tin oxide (ITO) electrode and utilized for KLK4 detection in human serum.
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Temel Bulgular & Veri:The ITO electrode was modified first by spin-coating of epoxy-substituted poly(thiophene) (Poly(TEpx)) conjugated polymer and thence anti-KLK4 antibody immobilization via the epoxy ends on the polymer.
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Sonuç & Katkı:The applicability of the immunosensor was tested in human serum samples and satisfactory results were obtained.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Kallikrein 4 · electrochemical impedance spectroscopy · prostate cancer

Abstract

In the present research, a label-free impedimetric kallikrein 4 (KLK4) biosensor was fabricated by using a low-cost and disposable indium tin oxide (ITO) electrode and utilized for KLK4 detection in human serum. The ITO electrode was modified first by spin-coating of epoxy-substituted poly(thiophene) (Poly(TEpx)) conjugated polymer and thence anti-KLK4 antibody immobilization via the epoxy ends on the polymer. The specific interaction between the anti-KLK4 antibody present on the working electrode surface and the KLK4 antigen caused increases in the impedimetric response, and the KLK4 antigen was determined in a linear concentration range from 0.025 to 12.5 pg/mL with a detection limit of 7.78 fg/mL. Furthermore, the developed biosensor had acceptable repeatability and reproducibility, long storage stability, and high selectivity to KLK4 antigen. The applicability of the immunosensor was tested in human serum samples and satisfactory results were obtained.

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Bu Makaleye Atıf Yap

Aydın, E. B. (2025). Label-free Electrochemical Immunosensor for Ultrasensitive Detection of KLK4 Using Conjugated Polymer Modified Sensing Platform. Hacettepe Journal of Biology and Chemistry, Volume: 53 Issue: 3. https://doi.org/10.15671/hjbc.1633456

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