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Dergiler / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 54 Issue: 3

Over Kanserinde Potansiyel Tanısal Gen Seti ve Yolak Değişiklikleri

Potential Diagnostic Gene Set and Pathway Alterations in Ovarian Cancer

Ece Gümüşoğlu-Acar(ISTANBUL UNIVERSITY, FACULTY OF SCIENCE)0000-0003-3807-0330
Berkcan Dogan(BURSA ULUDAG UNIVERSITY, SCHOOL OF MEDICINE)0000-0001-8061-8131
Mehmet Ulaş Bilir(ISTANBUL UNIVERSITY)0000-0001-8469-705X
Tugce Senturk Kirmizitas(ISTANBUL UNIVERSITY)0000-0001-6235-251X
Samet Topuz(ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE)0000-0002-9069-0185
Tuba Gunel(İstanbul Üniversitesi)0000-0003-3514-5210
Sayfa
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Yayın Tarihi
2026-06-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Fonksiyonel gen seti ve yolak analizi, g: profiler web sunucusu tarafından gerçekleştirilmiştir. ‘Genotype-Tissue Expression’ (GTEx) ve ‘The Cancer Genome Atlas’ (TCGA) veri setlerinde patolojik evre ve genel sağkalım analizi için ‘Gene Expression Profiling Interactive Analysis’ (GEPIA) kullanılmıştır. qRT-PCR sonuçları, FOS (P-değeri = 0,0089), MMP-9 (P-değeri = 0,0029) ve VEGFA (P-değeri = 0,0434) genlerinin ekspresyonunun arttığını göstermektedir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Over kanseri (OK), kadınlarda kansere bağlı ölümlerin sekizinci en yaygın nedenidir.
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Temel Bulgular & Veri:Bu çalışmada, OK patogenezi ve tanısında rol oynayabilecek çeşitli fonksiyonel genlerin taraması hedeflenmiştir. ‘Fos Proto-Oncogene, Activator Protein-1 Transcription Factor Subunit’ (FOS), ‘FOS Like 2, Activator Protein-1 Transcription Factor Subunit’ (FOSL2), ‘Activator Protein-1 Transcription Factor Subunit Jun Proto-Oncogene’ (JUN), ‘Matrix Metalloproteinases 2’ (MMP-2), ‘Matrix Metalloproteinases 9’ (MMP-9) and ‘Tissue Inhibitor of Metalloproteinase 2’ (TIMP-2) ve ‘Vascular Endothelial Growth Factor’ (VEGFA) genlerinin ekspresyon profilleri, sağlıklı over dokularında (n=10) ve yüksek dereceli seröz OK tümör dokularında (n=10) kantitatif gerçek zamanlı PCR (qRT-PCR) kullanılarak gerçekleştirilmiştir.
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Sonuç & Katkı:Fonksiyonel gen seti ve yolak analizi, g: profiler web sunucusu tarafından gerçekleştirilmiştir. ‘Genotype-Tissue Expression’ (GTEx) ve ‘The Cancer Genome Atlas’ (TCGA) veri setlerinde patolojik evre ve genel sağkalım analizi için ‘Gene Expression Profiling Interactive Analysis’ (GEPIA) kullanılmıştır. qRT-PCR sonuçları, FOS (P-değeri = 0,0089), MMP-9 (P-değeri = 0,0029) ve VEGFA (P-değeri = 0,0434) genlerinin ekspresyonunun arttığını göstermektedir.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:ovarian cancer · gene expression · diagnosis

Özet

Over kanseri (OK), kadınlarda kansere bağlı ölümlerin sekizinci en yaygın nedenidir. Bu çalışmada, OK patogenezi ve tanısında rol oynayabilecek çeşitli fonksiyonel genlerin taraması hedeflenmiştir. ‘Fos Proto-Oncogene, Activator Protein-1 Transcription Factor Subunit’ (FOS), ‘FOS Like 2, Activator Protein-1 Transcription Factor Subunit’ (FOSL2), ‘Activator Protein-1 Transcription Factor Subunit Jun Proto-Oncogene’ (JUN), ‘Matrix Metalloproteinases 2’ (MMP-2), ‘Matrix Metalloproteinases 9’ (MMP-9) and ‘Tissue Inhibitor of Metalloproteinase 2’ (TIMP-2) ve ‘Vascular Endothelial Growth Factor’ (VEGFA) genlerinin ekspresyon profilleri, sağlıklı over dokularında (n=10) ve yüksek dereceli seröz OK tümör dokularında (n=10) kantitatif gerçek zamanlı PCR (qRT-PCR) kullanılarak gerçekleştirilmiştir. Fonksiyonel gen seti ve yolak analizi, g: profiler web sunucusu tarafından gerçekleştirilmiştir. ‘Genotype-Tissue Expression’ (GTEx) ve ‘The Cancer Genome Atlas’ (TCGA) veri setlerinde patolojik evre ve genel sağkalım analizi için ‘Gene Expression Profiling Interactive Analysis’ (GEPIA) kullanılmıştır. qRT-PCR sonuçları, FOS (P-değeri = 0,0089), MMP-9 (P-değeri = 0,0029) ve VEGFA (P-değeri = 0,0434) genlerinin ekspresyonunun arttığını göstermektedir. FOSL2 (P-değeri = 0,0271), JUN (P-değeri = 0,0041) ve TIMP-2 (P-değeri = 0,0062) genlerinin ise ekspresyonu azalmıştır. Yolak zenginleştirme analizi relaksin sinyal yolağının en önemli (FDR=9.38e-08) biyolojik yolak olduğunu göstermiştir. FOSL2 ve TIMP-2, sırasıyla genel sağkalım ve patolojik evre analizinde önemlidir. Analiz edilen genler, kişiselleştirilmiş tanı ve tedavi için potansiyel taşımaktadır.

Abstract

Ovarian cancer (OC) is the eighth leading cause of cancer-related deaths among women. This study aimed to evaluate multiple functional genes involved in OC pathogenesis and diagnosis. The expression profiling of Fos Proto-Oncogene, Activator Protein-1 Transcription Factor Subunit (FOS), FOS Like 2, Activator Protein-1 Transcription Factor Subunit (FOSL2), Activator Protein-1 Transcription Factor Subunit Jun Proto-Oncogene (JUN), Matrix Metalloproteinases 2 (MMP-2), Matrix Metalloproteinases 9 (MMP-9) and Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) and Vascular Endothelial Growth Factor (VEGFA) was performed using quantitative real-time PCR (qRT-PCR) in healthy ovary tissues (n=10) and high-grade serous OC tissues (n=10). Functional gene set and pathway analysis were conducted by the g: profiler web server. Gene Expression Profiling Interactive Analysis (GEPIA) was used for pathological stage and overall survival analysis in the Genotype-Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA) datasets. qRT-PCR results revealed that FOS (P-value = 0.0089), MMP-9 (P-value = 0.0029), and VEGFA (P-value = 0.0434) were upregulated. FOSL2 (P-value = 0.0271), JUN (P-value = 0.0041), and TIMP-2 (P-value = 0.0062) were downregulated. Pathway enrichment analysis showed that the relaxin signaling pathway was the most significant (FDR=9.38e-08) biological pathway. The FOSL2 and TIMP-2 are significant in overall survival and pathological stage analysis, respectively. The analyzed genes may have the potential for personalized diagnosis and treatment.

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Bu Makaleye Atıf Yap

Gümüşoğlu-Acar, E., Dogan, B., Bilir, M. U., Kirmizitas, T. S., Topuz, S., Gunel, T. (2026). Over Kanserinde Potansiyel Tanısal Gen Seti ve Yolak Değişiklikleri. Hacettepe Journal of Biology and Chemistry, Volume: 54 Issue: 3. https://doi.org/10.15671/hjbc.1796263

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