KaydedilenlerDerginizi ekleyin

Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 2

Immunohistochemical Diagnosis of Aspergillosis in Adult Turkeys

Enver BEYTUT
Sayfa
99–104
Yayın Tarihi
2007-02-01
DOI
—
🏛️Bu makalenin bibliyografik verileri ve tam metin bağlantısı DergiPark üzerinden sağlanmıştır.
DergiPark Kaydı ↗
✨

Yapay Zeka Hızlı Bakış & Çıkarımlar

AI Insight
⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

In conclusion, even though fungal elements within the granulomas with histologic characteristics are indicative of the disease, immunohistochemistry should be applied to ensure a reliable diagnosis of aspergillosis in adult turkeys, and to differentiate it from other filamentous mycelia.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The aim of the present study was to apply immunohistochemistry, using a commercially available monoclonal antibody, for the diagnosis of aspergillosis in adult turkeys.
📊
Temel Bulgular & Veri:Eight adult turkeys were studied.
💡
Sonuç & Katkı:In conclusion, even though fungal elements within the granulomas with histologic characteristics are indicative of the disease, immunohistochemistry should be applied to ensure a reliable diagnosis of aspergillosis in adult turkeys, and to differentiate it from other filamentous mycelia.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Turkey · aspergillosis · immunohistochemistry

Özet

The aim of the present study was to apply immunohistochemistry, using a commercially available monoclonal antibody, for the diagnosis of aspergillosis in adult turkeys. Eight adult turkeys were studied. The lungs revealed grossly a few yellowish-white granulomas. Histopathologically, the granulomas were distributed in the pulmonary parenchyma or occasionally located around the airways. A few fungal hyphae were detected within the necrotic debris of the granulomas in the lungs using routine fungal stains. Immunohistochemical staining performed on lung sections from all of the turkeys disclosed fungal hyphae and their fragments clearly within the necrotic debris and within the cytoplasm of macrophages and multinucleated foreign-body giant cells in the granulomas. In conclusion, even though fungal elements within the granulomas with histologic characteristics are indicative of the disease, immunohistochemistry should be applied to ensure a reliable diagnosis of aspergillosis in adult turkeys, and to differentiate it from other filamentous mycelia.

Abstract

The aim of the present study was to apply immunohistochemistry, using a commercially available monoclonal antibody, for the diagnosis of aspergillosis in adult turkeys. Eight adult turkeys were studied. The lungs revealed grossly a few yellowish-white granulomas. Histopathologically, the granulomas were distributed in the pulmonary parenchyma or occasionally located around the airways. A few fungal hyphae were detected within the necrotic debris of the granulomas in the lungs using routine fungal stains. Immunohistochemical staining performed on lung sections from all of the turkeys disclosed fungal hyphae and their fragments clearly within the necrotic debris and within the cytoplasm of macrophages and multinucleated foreign-body giant cells in the granulomas. In conclusion, even though fungal elements within the granulomas with histologic characteristics are indicative of the disease, immunohistochemistry should be applied to ensure a reliable diagnosis of aspergillosis in adult turkeys, and to differentiate it from other filamentous mycelia.

🔖

Bu Makaleye Atıf Yap

BEYTUT, E. (2007). Immunohistochemical Diagnosis of Aspergillosis in Adult Turkeys. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 2, 99–104. https://ajindex.com/articles/125769

📄 Tam Metin PDF Okuyucu

📄

PDF görüntüleyici hazırlanıyor...

Kaynakça (13)

  1. Piérard, G.E., Arrese Estrada, J., Piérard-Franchimont, C., Thiry, A., Stynen, D.: Immunohistochemical expression of galactomannan in the cytoplasm of phagocytic cells during invasive aspergillosis. Am. J. Clin. Pathol., 1991; 96: 373-376. 2. Buckley, H.R., Richardson, M.D., Evans, E.G., Wheat, L.J.: Immunodiagnosis of invasive fungal infection. J. Med. Vet. Mycol., Jensen, H.E., Aalbæk, B., Lind, P., Frandsen, P.L., Krogh, H.V., Stynen, D.: Enzyme immunohistochemistry with mono- and polyclonal antibodies in the pathological diagnosis of systemic bovine mycoses. Acta Pathol. Microbiol. Immunol. Scand., 1993; 101: 505-516.
  2. Jensen, H.E., Christensen, J.P., Bisgaard, M., Nielsen, O.L.: Immunohistochemistry for the diagnosis of aspergillosis in turkey poults. Avian Pathol., 1997; 26: 5-18.
  3. Hsu, S.M., Raine, L., Fanger, H.: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem., 1981; 29: 577-580.
  4. Richard, J.L.: Aspergillosis. In: Calnek, B.W., Barnes, H., Beard, C.W., Reid, W.M., Yoder, H.W., Eds. Diseases of Poultry. Wolfe Publishing Ltd, London, 1991; 326-334.
  5. Singh, N., Grewal, G.S., Brar, R.S., Singh, B., Dhaliwal, A.S., Jand, S.K.: Outbreak of mycotic tracheitis in domestic fowl. Mycoses, 1993; 36: 65-67.
  6. Machin, K., Langelier, K.M., Lewis, R.J.: Aspergillosis outbreak in Stellar’s jays (Cyanocitta stelleri) from central Vancouver Island. Can. Vet. J., 1993; 34: 247-248.
  7. Hawkey, C.M., Pugsley, S.L., Knight, J.A.: Abnormal heterophils in a king shag with aspergillosis. Vet. Rec., 1984; 114: 322-324.
  8. Reece, R.L., Taylor, T.K., Dickson, D.B., Kerr, P.J.: Mycosis of commercial Japanese quail, ducks and turkeys. Aust. Vet. J., 1986; 63: 196-197.
  9. Beytut, E., Ozcan, K., Erginsoy, S.: Immunohistochemical detection of fungal elements in the tissues of goslings with pulmonary and systemic aspergillosis. Acta. Vet. Hung., 2004; 52: 71-84.
  10. Pal, M., Gangopadhyay, R.M., Prajapati, K.S.: Systemic aspergillosis in chicks due to Aspergillus flavus. Indian J. Anim. Sci., 1989; 59: 1074-1075.
  11. Pandita, A., Sadana, J.R., Asrani, R.K.: Studies on clinical signs and haematological alterations in pneumonic aspergillosis due to Aspergillus flavus in Japanese quail. Mycopathologia, 1991; 116: 119-123.
  12. Chute, H.L.: Diseases caused by fungi. In: Biester, H.E., Schwarte, L.H., Eds. Diseases of Poultry. The Iowa State University Press, Iowa, 1965; 494-502.
  13. Okoye, J.O.A., Ok