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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 5

Virulence Properties of Escherichia coli Isolated from Clinical Bovine Mastitis

Leyla GÜLER
Kadri GÜNDÜZ
Sayfa
361–365
Yayın Tarihi
2007-05-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Shiga toxin-producing E. coli (STEC), which is potentially pathogenic for humans, was not detected by PCR among the isolates from the cases of bovine mastitis.

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Amaç & Hipotez:The aim of this study was to detect the presence of the genes for some virulence factors in Escherichia coli isolates from clinical bovine mastitis.
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Temel Bulgular & Veri:A total of 100 E. coli isolates, each isolated from individual cows in different herds between 2000 and 2005, were examined.
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Sonuç & Katkı:Shiga toxin-producing E. coli (STEC), which is potentially pathogenic for humans, was not detected by PCR among the isolates from the cases of bovine mastitis.
🔬Tür:Araştırma Makalesi
📍Kapsam:2000 Verileri
⚙️Değişkenler:Escherichia coli · bovine mastitis · multiplex PCR

Özet

The aim of this study was to detect the presence of the genes for some virulence factors in Escherichia coli isolates from clinical bovine mastitis. A total of 100 E. coli isolates, each isolated from individual cows in different herds between 2000 and 2005, were examined. Two multiplex polymerase chain reaction (PCR) assays were used to detect the presence of the genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), heat-stable enterotoxin a (Sta), and F5 (K99), F41, and F17 fimbriae. In the PCR assays, 9 isolates were positive for the genes of F17 fimbriae and only 1 isolate was positive for the eaeA gene. The results demonstrated that none of the potential virulence factors investigated was commonly observed in E. coli isolates from bovine clinical mastitis. Shiga toxin-producing E. coli (STEC), which is potentially pathogenic for humans, was not detected by PCR among the isolates from the cases of bovine mastitis.

Abstract

The aim of this study was to detect the presence of the genes for some virulence factors in Escherichia coli isolates from clinical bovine mastitis. A total of 100 E. coli isolates, each isolated from individual cows in different herds between 2000 and 2005, were examined. Two multiplex polymerase chain reaction (PCR) assays were used to detect the presence of the genes encoding Shiga toxins 1 and 2 (stx1 and stx2), intimin (eaeA), heat-stable enterotoxin a (Sta), and F5 (K99), F41, and F17 fimbriae. In the PCR assays, 9 isolates were positive for the genes of F17 fimbriae and only 1 isolate was positive for the eaeA gene. The results demonstrated that none of the potential virulence factors investigated was commonly observed in E. coli isolates from bovine clinical mastitis. Shiga toxin-producing E. coli (STEC), which is potentially pathogenic for humans, was not detected by PCR among the isolates from the cases of bovine mastitis.

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Bu Makaleye Atıf Yap

GÜLER, L., GÜNDÜZ, K. (2007). Virulence Properties of Escherichia coli Isolated from Clinical Bovine Mastitis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 5, 361–365. https://ajindex.com/articles/125764

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Kaynakça (9)

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  2. Lam, T.J.G.M., Lipman, L.J.A., Schukken, Y.H., Gaastra, W., Brand, A.: Epidemiological characteristics of bovine clinical mastitis caused by Staphylococcus aureus and Escherichia coli studied by DNA fingerprinting. Am. J. Vet. Res., 1996; 57: 39- 42. Lehtolainen, T.: Escherichia coli mastitis: Bacterial factors and host response. Academic Dissertation. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland. 2004; Available: http://ethesis. helsinki.fi/julkaisut/ela/kliin/vk/lehtolainen/escheric.pdf Bean, A., Williamson, J., Cursons, R.T.: Virulence genes of Escherichia coli strains isolated from mastitic milk. J. Vet. Med. B Infect. Dis. Vet. Public Health, 2004; 51: 285-287.
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