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Dergiler / Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 19 Sayı: 2

G. diversifolia L. Bitki Ekstrelerinin İnsan Diş Eti Fibroblast Hücrelerinin Proliferasyonu ve Göçü Üzerindeki Etkileri

Effects of G. diversifolia L. Plant Extracts on Human Gingival Fibroblast Cell Proliferation and Migration

Özge Balpınar(ONDOKUZ MAYIS UNIVERSITY)0000-0003-4235-9241
Muhammed Sait Ertuğrul(ONDOKUZ MAYIS ÜNİVERSİTESİ)0000-0002-7885-5645
Büşra Tik(ONDOKUZ MAYIS UNIVERSITY)0000-0002-6104-6585
Mert Arslanbayrak(ONDOKUZ MAYIS UNIVERSITY)0000-0002-9584-6564
Ali Kemal Ayan(ONDOKUZ MAYIS UNIVERSITY)0000-0001-7981-6288
Sayfa
429–444
Yayın Tarihi
2026-08-31
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Ayrıca antioksidan durumunun doza bağlı olarak arttığı tespit edildi.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Girardinia diversifolia L. (Dev ısırgan otu), Urticaceae familyasından ısırgan otu (Urtica diocia L.) ile benzer özelliklere sahiptir.
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Temel Bulgular & Veri:Daha sonra, bitki ekstraktları, G. diversifolia L.'nin yara iyileşmesi üzerindeki etkilerini araştırmak için in vitro yara iyileştirme modelinde test edilmiştir.
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Sonuç & Katkı:Ayrıca antioksidan durumunun doza bağlı olarak arttığı tespit edildi.
🔬Tür:Deneysel Tarla Denemesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:linoleik asit · β-sitosterol · siringin

Özet

Girardinia diversifolia L. (Dev ısırgan otu), Urticaceae familyasından ısırgan otu (Urtica diocia L.) ile benzer özelliklere sahiptir. G. diversifolia L., hayvan ısırıklarından enfeksiyonlara kadar birçok tıbbi rahatsızlığın tedavisinde geleneksel tıpta kullanılan bir bitkidir. Ancak, dev ısırgan otunun tıbbi kullanımları üzerine literatür çalışmaları son derece sınırlıdır. Bu çalışmada, dev ısırgan otu fideleri yetiştirilmiş, uygun zamanlarda kök, yaprak ve çiçek örnekleri alınmış ve su ve metanol ekstraktları elde edilmiştir. Daha sonra, bitki ekstraktları, G. diversifolia L.'nin yara iyileşmesi üzerindeki etkilerini araştırmak için in vitro yara iyileştirme modelinde test edilmiştir. MTT sitotoksisite testi, ilgili ekstraktların hücre canlılığı üzerindeki etkilerini belirlemiştir. Ayrıca, yara dokusu gelişiminin oksidatif/antioksidatif özelliklerini ve biyokimyasal özelliklerini (IL-1β, TNF-α ve TGF-1β) belirlemek için ELISA testleri yapılmıştır. Sonuçlar IBM SPSS 21.0 programı kullanılarak analiz edildi. Analizler sonucunda, metanolde elde edilen G. diversifolia L. çiçek özütünün hücre çoğalmasını artırdığı, ancak hücre göçü üzerinde bir etkisi olmadığı gözlendi. IL1-β düzeylerinin kullanılan özüt dozuna bağlı olarak arttığı belirlendi. Hücre kültürü yara modeli testinde, göçün tespit edildiği metanol çiçek özütü 250 µg/mL ve 500 µg/mL gruplarında TNF-α ve TGF-β düzeylerinin arttığı, diğer gruplarda ise bir değişiklik olmadığı belirlendi. Ayrıca antioksidan durumunun doza bağlı olarak arttığı tespit edildi.

Abstract

Girardinia diversifolia L. (Giant nettle) has similar characteristics to nettle (Urtica diocia L.) in the Urticaceae family. G. diversifolia L. is a plant used in traditional medicine for many medical conditions ranging from animal bites to infections. However, studies on the medical uses of giant nettle in the literature are extremely limited. In this study, giant nettle seedlings were grown, and root, leaf, and flower samples were taken at appropriate times. Water and methanol extracts were then obtained. Subsequently, plant extracts were tested in an in vitro wound healing model to investigate the effects of G. diversifolia L. on wound healing. The MTT cytotoxicity test was used to determine the effects of the relevant extracts on cell viability. In addition, ELISA tests were performed to determine the oxidative/antioxidative properties, as well as the biochemical properties, of wound tissue development (IL-1β, TNF-α, and TGF-β). The results were analyzed using the IBM SPSS 21.0 program. As a result of the analyses, it was observed that the flower extract of G. diversifolia L. obtained in methanol increased cell proliferation but had no effect on cell migration. It was determined that IL-1β levels increased with the increase in extract dose. In the cell culture wound model test, it was determined that TNF-α and TGF-β levels increased in the methanol flower extract groups at 250 µg/mL and 500 µg/mL, where migration was detected, while no change was observed in the other groups. It was also determined that antioxidant status increased depending on the dose.

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Bu Makaleye Atıf Yap

Balpınar, Ö., Ertuğrul, M. S., Tik, B., Arslanbayrak, M., Ayan, A. K. (2026). G. diversifolia L. Bitki Ekstrelerinin İnsan Diş Eti Fibroblast Hücrelerinin Proliferasyonu ve Göçü Üzerindeki Etkileri. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 19 Sayı: 2, 429–444. https://doi.org/10.18185/erzifbed.1857668

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