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Dergiler / Sağlık Bilimlerinde İleri Araştırmalar Dergisi / 2026 / Volume: 8 Issue: 2

Beyin Metastazı için Gamma Knife Radyocerrahisi ile Tedavi Edilen Hastalarda Likit Biyopsinin Rolü

The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis

Büşra Karaçam(ISTANBUL UNIVERSITY, INSTITUTE OF HEALTH SCIENCES, GENETICS (DR))0000-0003-3824-8418
Imran Khan(BEZMI ALEM FOUNDATION UNIVERSITY, INSTITUTE OF LIFE SCIENCES AND BIOTECHNOLOGY, DEPARTMENT OF MOLECULAR BIOLOGY)0000-0002-6031-9465
Elif Burce Elbasan(BEZMI ALEM FOUNDATION UNIVERSITY, INSTITUTE OF LIFE SCIENCES AND BIOTECHNOLOGY, DEPARTMENT OF MOLECULAR BIOLOGY)0000-0001-9646-7083
Sadaf Mahfooz(BEZMI ALEM FOUNDATION UNIVERSITY, INSTITUTE OF LIFE SCIENCES AND BIOTECHNOLOGY, DEPARTMENT OF MOLECULAR BIOLOGY)0000-0001-6589-9377
Kerime Akdur(Bezmialem Vakif University, Schoof of Medicine, Department of Neurosurgery)0000-0003-3210-3621
Mustafa Aziz Hatiboğlu(BEZMI ALEM FOUNDATION UNIVERSITY, INSTITUTE OF LIFE SCIENCES AND BIOTECHNOLOGY, DEPARTMENT OF MOLECULAR BIOLOGY)0000-0002-4409-6337
Sayfa
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Yayın Tarihi
2025-06-30
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bulgular: BM hastalarında cfDNA ve eksozom düzeyleri sağlıklı gruba kıyasla daha yüksek bulundu.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Amaç: Beyin metastazlarının (BM) tedavi yöntemi cerrahi rezek siyon ve/veya radyoterapidir.
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Temel Bulgular & Veri:Bu çalışmada, GKR ile tedavi edilen BM hastalarında tanı koyma ve tedavi sonucunu öngörmede biyobelirteç olarak cfDNA seviyeleri, eksozom seviyeleri ve miR-208a ekspresyonunun rolünün araştırıl ması amaçlandı.
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Sonuç & Katkı:Bulgular: BM hastalarında cfDNA ve eksozom düzeyleri sağlıklı gruba kıyasla daha yüksek bulundu.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Brain metastasis · cfDNA · exosome

Özet

Amaç: Beyin metastazlarının (BM) tedavi yöntemi cerrahi rezek siyon ve/veya radyoterapidir. Gamma Knife radyocerrahisi (GKR) tedavisi, sağlıklı beyin dokusuna toksisiteyi önler. Bu nedenle, BM'li seçilmiş hastaların tedavisinde tercih edilen bir modalitedir. Bu çalışmada, GKR ile tedavi edilen BM hastalarında tanı koyma ve tedavi sonucunu öngörmede biyobelirteç olarak cfDNA seviyeleri, eksozom seviyeleri ve miR-208a ekspresyonunun rolünün araştırıl ması amaçlandı. Gereç ve Yöntemler: Bu çalışmaya GKR uygulanan beyin metastazlı dokuz hasta dahil edildi. Hastalardan farklı zaman aralıklarında (GKR öncesi, GKR sonrası 1. ay ve nüks döneminde) örnekler alındı. Yaş ve cinsiyet açısından eşleştirilmiş sekiz sağlıklı birey kontrol grubu olarak kullanıldı. Hasta ve kontrol grubundan cfDNA, ekso zom, serum miRNA ve serum ekzozomal miRNA izole edildi. Bulgular: BM hastalarında cfDNA ve eksozom düzeyleri sağlıklı gruba kıyasla daha yüksek bulundu. GKR sonrası cfDNA ve ekso zom düzeyleri azaldığı, ancak nüks döneminde tekrar arttığı tespit edildi. Serum miR-208a ve serum ekzozomal miR-208a ekspresy onu GKR sonrası artmış bulundu. Serum miR-208a ekspresyonu, akciğer kanseri hastalarında sağlıklı kontrollere ve akciğer kanseri olmayan hastalara kıyasla anlamlı derecede daha yüksek olduğu saptandı. Serum ekzozomal miR-208a ekspresyonu ise akciğer kanseri hastalarında sağlıklı kontrollere kıyasla anlamlı derecede yüksek bulundu. Sonuç: Bulgularımız, cfDNA ve eksozom düzeylerinin BM hastaları için tanısal belirteç olarak kullanılabileceğini göstermektedir.

Abstract

Objective: The treatment modality for brain metastases (BM) is surgical resection or radiotherapy. Gamma Knife radiosurgery (GKR) treatment prevents toxicity to healthy brain tissue; therefore, this modality is preferred for treating selected patients with BM. Our aim in this study was to investigate the role of cfDNA levels, exosome levels, and miR-208a expressions as biomarkers for diag nosis and predicting the outcome in patients with BM treated with GKR. Material and Methods: We included nine patients with brain metastasis who received GKR in this study. Samples were taken from patients at different time intervals (before GKR, 1 month after GKR, and at recurrence). Eight age- and gender-matched eight controls were used as healthy individuals. cfDNA, exosome, serum miRNA, and serum exosomal miRNA were isolated from the patient and control groups. Results: The levels of cfDNA and exosomes were higher in BM patients than in the healthy group. Exosome and cfDNA levels decreased after GKR and increased at recurrence. The expression of serum miR-208a and serum exosomal miR-208a was increased after GKR. The expression of serum miR-208a was significantly higher in patients with lung cancer than in healthy controls and non-lung cancer patients. The expression of serum exosomal miR-208a was found to be significantly higher in patients with lung cancer than in healthy controls. Conclusion: Our results showed that cfDNA and exosome levels might be used as diagnostic markers for BM patients.

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Bu Makaleye Atıf Yap

Karaçam, B., Khan, I., Elbasan, E. B., Mahfooz, S., Akdur, K., Hatiboğlu, M. A. (2025). Beyin Metastazı için Gamma Knife Radyocerrahisi ile Tedavi Edilen Hastalarda Likit Biyopsinin Rolü. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, Volume: 8 Issue: 2. https://doi.org/10.26650/JARHS2025-1671717

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