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Dergiler / Tarım Bilimleri Dergisi / 2026 / Volume: 32 Issue: 1

Inhibitory Impact of Pesticides on Glutathione Reductase Activity in Bonito Fish (Sarda sarda)

Ercan Soydan(ANKARA UNIVERSITY, FACULTY OF AGRICULTURE)0000-0002-9691-4434
Kübra Işık(ONDOKUZ MAYIS UNIVERSITY, FACULTY OF AGRICULTURE)0000-0002-7743-817X
Büşra Çevik(ONDOKUZ MAYIS UNIVERSITY, FACULTY OF AGRICULTURE)0000-0002-3683-5240
Seyit Batuhan Dudak(ONDOKUZ MAYIS UNIVERSITY, FACULTY OF AGRICULTURE)0009-0009-8835-296X
Deniz Ekinci(ONDOKUZ MAYIS UNIVERSITY, FACULTY OF AGRICULTURE)0000-0001-7849-4117
Sayfa
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Yayın Tarihi
2026-01-20
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Yapay Zeka Hızlı Bakış & Çıkarımlar

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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

These findings suggest that GR enzyme could serve as a potential biomarker for assessing the toxicological impacts of environmental pollutants for aquatic organisms.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Glutathione is a naturally occurring reducing agent that plays a critical role in protecting cells against oxidative stress.
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Temel Bulgular & Veri:This protective function is facilitated by antioxidant enzymes with the inclusion of glutathione peroxidase and glutathione reductase (GR; EC 1.8.1.7).
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Sonuç & Katkı:These findings suggest that GR enzyme could serve as a potential biomarker for assessing the toxicological impacts of environmental pollutants for aquatic organisms.
🔬Tür:Research Article
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Enzyme Purification · Glutathione Reductase · Pesticide

Abstract

Glutathione is a naturally occurring reducing agent that plays a critical role in protecting cells against oxidative stress. This protective function is facilitated by antioxidant enzymes with the inclusion of glutathione peroxidase and glutathione reductase (GR; EC 1.8.1.7). GR plays a key role in glutathione metabolism by continuously regenerating reduced glutathione (GSH), which is essential for combating oxidative stress within the cell. Therefore, GR activity is considered an important indicator of an organism’s defence capacity against environmental stressors, including heavy metals and pesticides. In this study, the GR enzyme was purified from the gill tissue of bonito and its biochemical response to pesticides was evaluated. After purification process, the optimum conditions for enzyme activity were determined as 200 mM KH₂PO₄ buffer at pH 6.5, and 2 mM NADPH as the substrate. The IC₅₀ values of the pesticides atrazine, oxamyl, and simazine were found to be 64.5 µM, 56.43 µM, and 53.45 µM, respectively. These findings suggest that GR enzyme could serve as a potential biomarker for assessing the toxicological impacts of environmental pollutants for aquatic organisms.

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Bu Makaleye Atıf Yap

Soydan, E., Işık, K., Çevik, B., Dudak, S. B., Ekinci, D. (2026). Inhibitory Impact of Pesticides on Glutathione Reductase Activity in Bonito Fish (Sarda sarda). Tarım Bilimleri Dergisi, Volume: 32 Issue: 1. https://doi.org/10.15832/ankutbd.1694737

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