Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2013 / Cilt: 37 - Sayı: 1

Microsatellite analysis for parentage testing of the Arabian horse breed from Syria

Sayfa
9–14
Yayın Tarihi
2013-01-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The results of this study indicate no serious loss of heterozygosity and confirm that 13 STR markers can be used in parentage testing with high efficiency for the Arabian horses from Syria.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Parentage testing is very important for genetically determining the accuracy of the pedigree of domestic animals.
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Temel Bulgular & Veri:The purpose of this study was to analyze the usefulness of 16 STR markers for routine parentage testing in 94 samples from registered and nonregistered horses collected from Syria.
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Sonuç & Katkı:The results of this study indicate no serious loss of heterozygosity and confirm that 13 STR markers can be used in parentage testing with high efficiency for the Arabian horses from Syria.
🔬Tür:Araştırma Makalesi
📍Kapsam:94 samples
⚙️Değişkenler:Key words: Arabian horse · parentage testing · microsatellite

Özet

Parentage testing is very important for genetically determining the accuracy of the pedigree of domestic animals. The Arabian horse population in Syria might be expected to have a high level of homozygosity because the traditional method of breeding results in such a small population. For this reason, using a set of highly polymorphic markers such as microsatellites is required for parentage testing. The purpose of this study was to analyze the usefulness of 16 STR markers for routine parentage testing in 94 samples from registered and nonregistered horses collected from Syria. The mean number of alleles was 5.69 per locus in the registered group and 7.69 in the nonregistered group. The nonregistered group had a mean polymorphic information content (PIC) of 0.715, which was significantly higher than the PIC in the registered group. The individual probability of exclusion per locus ranged from 32% for HTG7 to 80% for ASB17 in the registered group and 41% for HTG7 to 84% for ASB17 in the nonregistered group. The results of this study indicate no serious loss of heterozygosity and confirm that 13 STR markers can be used in parentage testing with high efficiency for the Arabian horses from Syria.

Abstract

Parentage testing is very important for genetically determining the accuracy of the pedigree of domestic animals. The Arabian horse population in Syria might be expected to have a high level of homozygosity because the traditional method of breeding results in such a small population. For this reason, using a set of highly polymorphic markers such as microsatellites is required for parentage testing. The purpose of this study was to analyze the usefulness of 16 STR markers for routine parentage testing in 94 samples from registered and nonregistered horses collected from Syria. The mean number of alleles was 5.69 per locus in the registered group and 7.69 in the nonregistered group. The nonregistered group had a mean polymorphic information content (PIC) of 0.715, which was significantly higher than the PIC in the registered group. The individual probability of exclusion per locus ranged from 32% for HTG7 to 80% for ASB17 in the registered group and 41% for HTG7 to 84% for ASB17 in the nonregistered group. The results of this study indicate no serious loss of heterozygosity and confirm that 13 STR markers can be used in parentage testing with high efficiency for the Arabian horses from Syria.

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Bu Makaleye Atıf Yap

KHANSHOUR, A. M., CONANT, E. K., JURAS, R., COTHRAN, E. G. (2013). Microsatellite analysis for parentage testing of the Arabian horse breed from Syria. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 1, 9–14. https://ajindex.com/articles/122348

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  1. Locus Chromosome Reference ASB17 2 (18) LEX33 4 (22) HMS2 10 (19) VHL20 30 (23) LEX3 X (20) HTG6 15 (24) ASB23 3 (21) AHT4 24 (25) ASB2 15 (18) AHT5 8 (25) HMS7 1 (19) HTG4 9 (24) HMS3 9 (19) HMS6 4 (19) HTG10 21 (14) HTG7 4 (14) Results The PCR amplicons ranged between 93 bp and 211 bp in size. As shown in Table 2, the total Na was 91 in the registered group, with a mean of 5.69 per locus, and 123 alleles in the nonregistered group, with a mean of 7.69. The Na per locus ranged from 3 for HTG6 and HTG7 to 8 for ASB2 in the registered group, while in the nonregistered group, the Na ranged from 4 for HTG7 to 14 for ASB17. The Ne varied from 1.86 for HTG7 to 5.464 for ASB17 in the registered group and from 2.141 for HTG7 to 988 for ASB17 in the nonregistered group. The mean Ne was 3.747 in the registered group and 4.476 in the nonregistered group. The observed heterozygosity per locus in the registered group varied from 0.362 for HTG7 to 0.915 for LEX33 and from 0.469 for HMS2 to 0.878 for ASB17 for the nonregistered group, with means of 0.694 and 0.711, respectively. The lowest PIC value for both groups was for HTG7 (0.385 in the registered group and 0.454 in the nonregistered group), while the highest value was for ASB17 (0.781 in the registered group and 0.803 in the nonregistered group). The mean PIC was 0.657 in the registered group and 0.715 in the nonregistered group. The individual PE ranged from 32% at the HTG7 locus to 80% at ASB17 for the registered group and 41% at the HTG7 locus to 84% at ASB17 for the nonregistered group. The CPEs for all of the loci were more than 99.999% in each group. The Figure shows the CPE values for both groups as a function of the number of microsatellite loci. Discussion How informative a locus is depends upon the Na exhibited by the locus and the frequency distribution of these alleles in the population (8). The mean Na in the present study was higher than that seen in Sorraia, a breed that has a high level of inbreeding (29). Values of diversity statistics similar to those observed here for the Arabian breed have been Table Number of alleles (Na), number of effective alleles (Ne), observed (Ho) and expected (He) heterozygosity, polymorphic information content (PIC), probability of exclusion (PE), and combined probabilities of exclusion (CPE) for registered and nonregistered Arabian horses. Group Registered Nonregistered Locus Na Ne Ho He PIC PE Na Ne Ho He PIC PE ASB17 7 4 0.872 0.817 0.781 0.800 14 9 0.878 0.833 0.80