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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2008 / Cilt: 32 - Sayı: 2

Comparative Results of Survival of Vitrified Biopsied Goat Embryos and Mouse Morulae

Aqin CHEN
Rui ZHANG
Songdong YU
Sayfa
93–97
Yayın Tarihi
2008-02-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

It may be concluded that goat embryos were vitrified safely in an ethylene glycol-based solution (EFS30) by the 2-step method.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:The purpose of this study was to find an efficient cryoprotectant and a suitable procedure for vitrification of mouse morulae.
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Temel Bulgular & Veri:Mouse morulae were exposed, in 1- or 2-step, to the ethylene glycol (EG)-based solutions EFS30 or EDFS30, which contained 30% EG and 15% EG plus 15% dimethylsulphoxide (DMSO), respectively, in phosphate buffer saline (PBS) containing 30% (w/v) Ficoll and 0.5 M sucrose, cooled in liquid nitrogen and warmed up rapidly.
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Sonuç & Katkı:It may be concluded that goat embryos were vitrified safely in an ethylene glycol-based solution (EFS30) by the 2-step method.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Mouse morulae · biopsied goat embryos · vitrification

Özet

The purpose of this study was to find an efficient cryoprotectant and a suitable procedure for vitrification of mouse morulae. The method found with mouse morulae was then applied to the cryopreservation of biopsied goat embryos. Mouse morulae were exposed, in 1- or 2-step, to the ethylene glycol (EG)-based solutions EFS30 or EDFS30, which contained 30% EG and 15% EG plus 15% dimethylsulphoxide (DMSO), respectively, in phosphate buffer saline (PBS) containing 30% (w/v) Ficoll and 0.5 M sucrose, cooled in liquid nitrogen and warmed up rapidly. After warming, the rate of the hatched blastocyst formation in mouse morulae vitrified with EFS30 (54.0%) by the 2-step method was significantly higher compared to those vitrified with EFS30 (30.4%) and EDFS30 (27.7%) by the 1-step method (P < 0.05). According to those results, biopsied goat embryos were vitrified in EFS30 using the 2-step method. The results showed that the developmental blastocyst rate of the post-thawed embryos was 47.1%. It may be concluded that goat embryos were vitrified safely in an ethylene glycol-based solution (EFS30) by the 2-step method.

Abstract

The purpose of this study was to find an efficient cryoprotectant and a suitable procedure for vitrification of mouse morulae. The method found with mouse morulae was then applied to the cryopreservation of biopsied goat embryos. Mouse morulae were exposed, in 1- or 2-step, to the ethylene glycol (EG)-based solutions EFS30 or EDFS30, which contained 30% EG and 15% EG plus 15% dimethylsulphoxide (DMSO), respectively, in phosphate buffer saline (PBS) containing 30% (w/v) Ficoll and 0.5 M sucrose, cooled in liquid nitrogen and warmed up rapidly. After warming, the rate of the hatched blastocyst formation in mouse morulae vitrified with EFS30 (54.0%) by the 2-step method was significantly higher compared to those vitrified with EFS30 (30.4%) and EDFS30 (27.7%) by the 1-step method (P < 0.05). According to those results, biopsied goat embryos were vitrified in EFS30 using the 2-step method. The results showed that the developmental blastocyst rate of the post-thawed embryos was 47.1%. It may be concluded that goat embryos were vitrified safely in an ethylene glycol-based solution (EFS30) by the 2-step method.

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Bu Makaleye Atıf Yap

CHEN, A., ZHANG, R., YU, S. (2008). Comparative Results of Survival of Vitrified Biopsied Goat Embryos and Mouse Morulae. Turkish Journal of Veterinary and Animal Sciences, Cilt: 32 - Sayı: 2, 93–97. https://ajindex.com/articles/122163

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Kaynakça (10)

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