Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 6

Allelic Frequency of Kappa-Casein and Beta-Lactoglobulin in Indian Crossbred (Bos taurus X Bos indicus) Dairy Bulls

Sayfa
399–402
Yayın Tarihi
2007-06-01
DOI
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Kappa-casein and beta-lactoglobulin are useful markers for milk production traits on which bulls can be evaluated and selected for future breeding programmes.

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Amaç & Hipotez:A polymerase chain reaction - restriction fragment length polymorphism test was performed on DNA samples extracted from blood samples of 256 Holstein Friesian crossbred and 112 from Jersey crossbred bulls/bull calves to detect allelic frequency of the bovine kappa-casein and beta-lactoglobulin gene responsible for milk production traits.
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Temel Bulgular & Veri:A 350 bp fragment of kappa-casein and a 247 bp fragment of beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively.
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Sonuç & Katkı:Kappa-casein and beta-lactoglobulin are useful markers for milk production traits on which bulls can be evaluated and selected for future breeding programmes.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Jersey · Holstein Friesian · Kappa-casein

Özet

A polymerase chain reaction - restriction fragment length polymorphism test was performed on DNA samples extracted from blood samples of 256 Holstein Friesian crossbred and 112 from Jersey crossbred bulls/bull calves to detect allelic frequency of the bovine kappa-casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa-casein and a 247 bp fragment of beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. In the study, 2 types of alleles A and B and 3 types of genotypes AA, BB, and AB for kappa-casein and beta-lactoglobulin were observed. The results indicated that allelic frequency of kappa-casein for A-allele (0.66) was higher compared to B-allele (0.34). Similarly, B-allele frequency (0.72) of beta-lactoglobulin was much higher compared to A-allele (0.27) in crossbred dairy bulls. Kappa-casein and beta-lactoglobulin are useful markers for milk production traits on which bulls can be evaluated and selected for future breeding programmes.

Abstract

A polymerase chain reaction - restriction fragment length polymorphism test was performed on DNA samples extracted from blood samples of 256 Holstein Friesian crossbred and 112 from Jersey crossbred bulls/bull calves to detect allelic frequency of the bovine kappa-casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa-casein and a 247 bp fragment of beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. In the study, 2 types of alleles A and B and 3 types of genotypes AA, BB, and AB for kappa-casein and beta-lactoglobulin were observed. The results indicated that allelic frequency of kappa-casein for A-allele (0.66) was higher compared to B-allele (0.34). Similarly, B-allele frequency (0.72) of beta-lactoglobulin was much higher compared to A-allele (0.27) in crossbred dairy bulls. Kappa-casein and beta-lactoglobulin are useful markers for milk production traits on which bulls can be evaluated and selected for future breeding programmes.

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Bu Makaleye Atıf Yap

PATEL, R. K., CHAUHAN, J. B., SINGH, K. M., SONI, K. J. (2007). Allelic Frequency of Kappa-Casein and Beta-Lactoglobulin in Indian Crossbred (Bos taurus X Bos indicus) Dairy Bulls. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 6, 399–402. https://ajindex.com/articles/122144

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