Moreover, MDA levels were significantly decreased as a result of melatonin treatment (P < 0.01).
📌 3 Maddede Bu Makale
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Amaç & Hipotez:Effects of melatonin on the levels of antioxidant enzymes including catalase (CAT), peroxidase (POD), glutathione reductase (GRd), and malondialdehyde (MDA), a lipid peroxidation marker, in erythrocytes of rainbow trout (Oncorhynchus mykiss) were investigated.
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Temel Bulgular & Veri:The results showed that melatonin statistically activated the CAT, POD, and GRd activity (P < 0.05).
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Sonuç & Katkı:Moreover, MDA levels were significantly decreased as a result of melatonin treatment (P < 0.01).
Effects of melatonin on the levels of antioxidant enzymes including catalase (CAT), peroxidase (POD), glutathione reductase (GRd), and malondialdehyde (MDA), a lipid peroxidation marker, in erythrocytes of rainbow trout (Oncorhynchus mykiss) were investigated. The results showed that melatonin statistically activated the CAT, POD, and GRd activity (P < 0.05). Moreover, MDA levels were significantly decreased as a result of melatonin treatment (P < 0.01).
Abstract
Effects of melatonin on the levels of antioxidant enzymes including catalase (CAT), peroxidase (POD), glutathione reductase (GRd), and malondialdehyde (MDA), a lipid peroxidation marker, in erythrocytes of rainbow trout (Oncorhynchus mykiss) were investigated. The results showed that melatonin statistically activated the CAT, POD, and GRd activity (P < 0.05). Moreover, MDA levels were significantly decreased as a result of melatonin treatment (P < 0.01).
Halliwell, B., Chirico, S.: Lipid peroxidation: its mechanism, measurement, and significance. Am. J. Clin. Nutr., 1993; 57: 715-725.
Kang, C.R., Sweeter, S., Boylan, L.M., Spallholz, J.E.: Oxygen toxicity, biological defense systems and immunity: a historical perspective. J. Nutr. Immunol., 1994; 3: 51-84.
Filho, D.W., Giulivi, C., Boveris, A.: Antioxidant defences in marine fish I: Teleosts. Comp. Biochem. Physiol. C Comp. Pharmacol. Toxicol., 1993; 106: 409-413.
Amano, M., Iigo, M., Ikuta, K., Kitamura, S., Yamada, H., Yamamori, K.: Roles of melatonin in gonadal maturation of underyearling precocious male Masu salmon. Gen. Comp. Endocrinol., 2000; 120: 190-197.
Bornestaf, C., Mayer, I., Borg, B.: Melatonin and maturation pace in female three-spined stickleback Gasterosteus aculeatus. Gen. Comp. Endocrinol., 2001; 122: 341-348. ARENA (Applied Research Ethics National Association): Institutional Animal Care and Use Committee Guidebook. 2nd edn., National Academy of Sciences, Boston. 2002; 121-125.
Aebi, H.: Catalase in vitro. Methods Enzymol., 1984; 105: 121- 126. Shannon, L.M., Kay, E., Lew, J.Y.: Peroxidase isoenzymes from horseradish roots. I. Isolation and physical properties. J. Biol. Chem., 1996; 241: 2166-2172.
Hunter, M.I.S, Nlemadim, B.C., Davidson, D.L.W.: Lipid peroxidation products and antioxidant proteins in plasma and cerebrospinal fluid from multiple sclerosis patients. Neurochem. Res., 1985; 10: 1645-1652.
Reiter, R.J., Tan, D.X., Qi, W.B., Manchester, L.C., Karbownik, M., Calvo, J.R.: Pharmacology and physiology of melatonin in the reduction of oxidative stress in vivo. Biol. Signals Recept., 2000; 9: 160-171.
Rosen, J., Than, N.N., Koch, D., Poeggeler, B., Laatsch, H. Hardeland, R.: Interactions of melatonin and its metabolites with the ABTS cation radical: extension of the radical scavenger cascade and formation of a novel class of oxidation products, C2-substituted 3-indolinones. J. Pineal Res., 2006; 41: 374-381.
Reiter, R., Tang, L., Garcia, J.J., Muñoz-Hoyos, A.: Pharmacological actions of melatonin in oxygen radical pathophysiology. Life Sci., 1997; 60: 2255-2271. Tomás-Zapico, C., Coto-Montes, A.: A proposed mechanism to explain the stimulatory effect of melatonin on antioxidative enzymes. J. Pineal Res., 2005; 39: 99-104.