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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2010 / Cilt: 34 - Sayı: 2

Identification of Babesia and Theileria species in small ruminants in Central Anatolia (Turkey) via reverse line blotting

Abdullah İNCİ
Anıl İÇA
Alparslan YILDIRIM
Önder DÜZLÜ
Sayfa
205–210
Yayın Tarihi
2010-02-01
DOI
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As a result, the molecular prevalence of B. ovis was as follows: 2.9% in sheep (12/421), 2% in goats (3/152), and 2.6% in the total study population (15/573), versus 42% in sheep (177/421), 11.2% in goats (17/152), and 33.9% in the total population (194/573) for T. ovis.

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Amaç & Hipotez:This study was conducted between January 2006 and September 2008 to identify Babesia and Theileria species in sheep and goats in Central Anatolia via reverse line blotting (RLB).
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Temel Bulgular & Veri:In total, 421 sheep and 152 goats were randomly selected from herds located in Kayseri, Yozgat, and Sivas provinces (all in central Turkey).
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Sonuç & Katkı:As a result, the molecular prevalence of B. ovis was as follows: 2.9% in sheep (12/421), 2% in goats (3/152), and 2.6% in the total study population (15/573), versus 42% in sheep (177/421), 11.2% in goats (17/152), and 33.9% in the total population (194/573) for T. ovis.
🔬Tür:Araştırma Makalesi
📍Kapsam:2006 Verileri
⚙️Değişkenler:Key words: Theileria sp. · Babesia sp. · small ruminants

Özet

This study was conducted between January 2006 and September 2008 to identify Babesia and Theileria species in sheep and goats in Central Anatolia via reverse line blotting (RLB). In total, 421 sheep and 152 goats were randomly selected from herds located in Kayseri, Yozgat, and Sivas provinces (all in central Turkey). The amplified hypervariable V4 region of the 18S rRNA gene of ovine piroplasm species, including Theileria lestoquardi, T. ovis, Babesia ovis, B. motasi, and B. crassa, was hybridized against species-specific probes. Theileria ovis and B. ovis were observed in the study animals. Among the 573 small ruminants included in the study, 182 (43.2%) of 421 sheep and 18 (11.8%) of 152 goats, (in total 200 [34.9%]) were catch-all positive based on RLB. Among the sheep, T. ovis was observed in 170 (40.4%), B. ovis in 5 (1.2%), and B. ovis + T. ovis in 7 (1.7%). Theileria ovis, B. ovis, and B. ovis + T. ovis were observed in 15 (9.9%), in 1 (0.7%), and in 2 (1.3%) of the examined goats, respectively. As a result, the molecular prevalence of B. ovis was as follows: 2.9% in sheep (12/421), 2% in goats (3/152), and 2.6% in the total study population (15/573), versus 42% in sheep (177/421), 11.2% in goats (17/152), and 33.9% in the total population (194/573) for T. ovis.

Abstract

This study was conducted between January 2006 and September 2008 to identify Babesia and Theileria species in sheep and goats in Central Anatolia via reverse line blotting (RLB). In total, 421 sheep and 152 goats were randomly selected from herds located in Kayseri, Yozgat, and Sivas provinces (all in central Turkey). The amplified hypervariable V4 region of the 18S rRNA gene of ovine piroplasm species, including Theileria lestoquardi, T. ovis, Babesia ovis, B. motasi, and B. crassa, was hybridized against species-specific probes. Theileria ovis and B. ovis were observed in the study animals. Among the 573 small ruminants included in the study, 182 (43.2%) of 421 sheep and 18 (11.8%) of 152 goats, (in total 200 [34.9%]) were catch-all positive based on RLB. Among the sheep, T. ovis was observed in 170 (40.4%), B. ovis in 5 (1.2%), and B. ovis + T. ovis in 7 (1.7%). Theileria ovis, B. ovis, and B. ovis + T. ovis were observed in 15 (9.9%), in 1 (0.7%), and in 2 (1.3%) of the examined goats, respectively. As a result, the molecular prevalence of B. ovis was as follows: 2.9% in sheep (12/421), 2% in goats (3/152), and 2.6% in the total study population (15/573), versus 42% in sheep (177/421), 11.2% in goats (17/152), and 33.9% in the total population (194/573) for T. ovis.

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Bu Makaleye Atıf Yap

İNCİ, A., İÇA, A., YILDIRIM, A., DÜZLÜ, Ö. (2010). Identification of Babesia and Theileria species in small ruminants in Central Anatolia (Turkey) via reverse line blotting. Turkish Journal of Veterinary and Animal Sciences, Cilt: 34 - Sayı: 2, 205–210. https://ajindex.com/articles/122041

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Kaynakça (6)

  1. Bishop, R., Musoke, A., Morzaria, S., Gardner, M., Nene, V.: Theileria: intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks. Parasitology, 2004; 129 (Suppl.): 271-283. 2. Mehlhorn, H., Shein, E.: The piroplasms: life cycle and sexual stages. Adv. Parasitol., 1984; 23: 37-103. 3. Preston, P.M.: Theilerioses. In: Service, M.W., Ed., Encyclopedia of Arthropod-Transmitted Infections of Man and Domesticated Animals. CABI Publishing, Wallingford, 2001; 487-502. 4. Uilenberg, G.: Babesiosis. In: Service, M.W., Ed., Encyclopedia of Arthropod-Transmitted Infections of Man and Domesticated Sparagano, O.A., de Vos, A.P., Paoletti, B., Cammà, C., de Santis, P., Otranto, D., Giangaspero, A.: Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. J. Vet. Diagn. Invest., 2003; 15: 527-534.
  2. Altay, K., Aktas, M., Dumanli, N., Aydin, M.F.: Evaluation of a PCR and comparison with RLB for detection and differentiation of Theileria sp. MK and other Theileria and Babesia species of small ruminants. Parasitol. Res., 2008; 103: 319-323.
  3. Criado, A., Martinez, J., Buling, A., Barba, J.C., Merino, S., Jefferies, R., Irwin, P.J.: New data on epizootiology and genetics of piroplasms based on sequences of small ribosomal subunit and cytochrome b genes. Vet. Parasitol., 2006; 142: 238-247.
  4. Gubbels, M.J., Yin, H., van der Weide, M., Qi, B., Nijman, I.J., Guangyuan, L., Jongejan, F.: Molecular characterisation of the Theileria buffeli / orientalis group. Int. J. Parasitol., 2000; 30: 943- 952. Schnittger, L., Yin, H., Gubbels, M.J., Beyer, D., Niemann, S., Jongejan, F., Ahmed, J.S.: Phylogeny of sheep and goat Theileria and Babesia parasites. Parasitol. Res., 2003; 91: 398-406.
  5. İnci, A., Yukarı B.A., Sayın, F.: Study on babesiosis and theileriosis agents detected in some sheep and goat flocks using microscopic examination in Çankırı region. Ankara Üniv. Vet. Fak. Derg., 1998; 45: 105-113. (article in Turkish with an abstract in English) İnci, A., Karaer, Z., İça, A.: Babesiosis in sheep and goats around Kayseri. Fırat Üniv. Sağ. Bil. Derg., 2002; 16: 79-83. (article in Turkish with an abstract in English) Çakmak, A., İnci, A., Karaer, Z.: Seroprevalence of Babesia ovis in sheep and goats in Çankırı region. Türkiye Parazitol. Derg., 1998; 22: 73-76.
  6. Sayin, F., Dincer, S., Karaer, Z., Cakmak, A., Yukari, B.A., Eren, H., Deger, S., Nalbantoglu, S.: Status of the tick-borne diseases in sheep