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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2012 / Cilt: 36 - Sayı: 1

Serum protein fractions in Brazilian-breed donkeys using agarose gel electrophoresis

Paulo Henrique CAVALCANTE
Andréa Cristina C. SILVA
Sidnei M. SAKAMOTO
Benito SOTO BLANCO
Sayfa
9–12
Yayın Tarihi
2012-01-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Sex did not affect the serum protein levels.

📌 3 Maddede Bu Makale

🎯
Amaç & Hipotez:The values for the serum protein profiles of horses are available in many studies.
📊
Temel Bulgular & Veri:Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses.
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Sonuç & Katkı:Sex did not affect the serum protein levels.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Albumin · donkeys · globulins

Özet

The values for the serum protein profiles of horses are available in many studies. However, only one study has characterized the electrophoretic migration profile of plasma protein in donkeys. The present study aimed to determine by agarose gel electrophoresis the normal values for serum protein fractions in erratic Brazilian-breed donkeys and to compare these values with those of the serum proteins of horses. Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses. The serum levels of the total proteins were determined by the biuret method. The separation and determination of the values of serum protein fractions were performed by means of agarose gel electrophoresis and the analysis of the data was done using specific software. The results were compared using an unpaired t-test, with the level of statistical significance set at P < 0.05. A total of 7 protein fractions were found: albumin and a1-, a2-, b1-, b2-, g1-, and g2-globulins. The results showed that in the evaluated donkeys the levels of a2-globulins were higher than those of a1-globulins and that the levels of g1-globulins were higher than those of g2-globulins. Furthermore, the sexual interference in the concentrations of proteins and their fractions revealed that no sex-related difference was present. The serum concentrations of protein fractions from the donkeys were similar to those of horses. Sex did not affect the serum protein levels.

Abstract

The values for the serum protein profiles of horses are available in many studies. However, only one study has characterized the electrophoretic migration profile of plasma protein in donkeys. The present study aimed to determine by agarose gel electrophoresis the normal values for serum protein fractions in erratic Brazilian-breed donkeys and to compare these values with those of the serum proteins of horses. Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses. The serum levels of the total proteins were determined by the biuret method. The separation and determination of the values of serum protein fractions were performed by means of agarose gel electrophoresis and the analysis of the data was done using specific software. The results were compared using an unpaired t-test, with the level of statistical significance set at P < 0.05. A total of 7 protein fractions were found: albumin and a1-, a2-, b1-, b2-, g1-, and g2-globulins. The results showed that in the evaluated donkeys the levels of a2-globulins were higher than those of a1-globulins and that the levels of g1-globulins were higher than those of g2-globulins. Furthermore, the sexual interference in the concentrations of proteins and their fractions revealed that no sex-related difference was present. The serum concentrations of protein fractions from the donkeys were similar to those of horses. Sex did not affect the serum protein levels.

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Bu Makaleye Atıf Yap

CAVALCANTE, P. H., SILVA, A. C. C., SAKAMOTO, S. M., BLANCO, B. S. (2012). Serum protein fractions in Brazilian-breed donkeys using agarose gel electrophoresis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 36 - Sayı: 1, 9–12. https://ajindex.com/articles/121868

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Kaynakça (4)

  1. Thomas, ].S.: Protein electrophoresis. In: Feldman, B.F., Zinkl, 1.G., 1ain, N.C., Eds. Schalm’s Veterinary Hematology. 5th ed., Blackwell Publ., Ames, 2006; 899—903. Eckersall, P.D.: Proteins, proteomics, and the dysproteinemias. In: Kaneko, 1.1., Harvey, ].W., Bruss, M.L., Eds. Clinical Biochemistry of Domestic Animals. 6th ed., Academic Press, Burlington. 2008; 117-155.
  2. Carapeto, M.V., Barrera, R., Mafıe, M.C., Zaragoza, C.: Serum a—globulin fraction in horses is related to changes in the acute phase proteins. ]. Equine Vet. Sci., 2006; 26: 120—127. Agricola, R., Carvalho, H., Barbosa, M., Pereira, M., Medeiros, ].A.S., Ferreira—Dias, G.: Blood lymphocyte subpopulations, neutrophil phagocytosis and proteinogram during late pregnancy and postpartum in mares. Reprod. Dom. An., 2008; 43: 212—217. Kaneko, ].]., Harvey, ].W, Bruss, M.L.: Clinical Biochemistry of Domestic Animals. 6 th ed., Academic Press, Burlington. 2008; 908—915. Caldin, M., Furlanello, T., Solano—Gallego, L., Lorenzi, D.D., Carli, E., Tasca, S., Lubas, G.: Reference ranges for haematology, biochemical proŞle and electrophoresis in a single herd of Ragusana donkeys from Sicily (Italy). Comp. Clin. Path., 2005; 14: 5—12. Mori, E., Fernandes, W.R., Mirandola, R.M.S., Kubo, G., Ferreira, R.R., Oliveira, ].V., Gacek, F.: Reference values on serum biochemical parameters of Brazilian donkey (Equus usinus) breed. ]. Equine Vet. Sci., 2003; 23: 358-364.
  3. Pitel, P., Moulin, M., Valette, ].P., Dumontier, S., Petit, L., Fortier, G., Courouce—Malblanc, A.: Approach of hematological and blood biochemistry values in two breeds of donkeys. Pratique Vétérinaire Equine, 2006; 38(149): 19—25. (article in French with an abstract in English). revealed 7 protein fractions. The levels of (ıı—2 globulins were higher than those of (IL-1 globulins, and the concentration of yl-globulins was higher than that of y2- globulins. Sex did not affect the serum protein levels. French, 1.M., Patrick, V.H.: Reference values for physiological, haematological and biochemical parameters in domestic donkeys (Equus usinus). Eq. Vet. Educ., 1995; 7, 33-35. Zinkl, 1.G., Mae, D., Guzman, P., Farver, T.B., Humble, 1.A.: Reference ranges and the inşuence of age and sex on hematologic and serum biochemical values in donkeys (Equus minus). Am. 1. Vet. Res., 1990; 51: 408-413.
  4. Gupta, A.K., Varshney, I.P., Uppal, P.K.: Comparative studies on biochemical indices in different breeds of equines. Indian Vet. ]., 1994; 71: 26—30. Morris, D.D.,