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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2012 / Cilt: 36 - Sayı: 6

Establishment and biological research of the Jining Grey goat fibroblast line

Chunyu BAI
Dianjun WANG
Xiaohua SU
Minghai ZHANG
Weijun GUAN
Yuehui MA
Sayfa
659–667
Yayın Tarihi
2012-06-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics, and somatic cloning.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique.
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Temel Bulgular & Veri:The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h.
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Sonuç & Katkı:This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics, and somatic cloning.
🔬Tür:Araştırma Makalesi
📍Kapsam:32 samples
⚙️Değişkenler:Key words: Jining Grey goat · fibroblast cell bank · cryogenic preservation

Özet

Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h. The diploid accounted for 98.35%, the isozyme analysis of the lactic dehydrogenase and malic dehydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fluorescent protein genes were high, which supported the opinion that exogenous genes could be effectively expressed in the cells. This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics, and somatic cloning.

Abstract

Using Jining Grey goat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primary explant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately 48 h. The diploid accounted for 98.35%, the isozyme analysis of the lactic dehydrogenase and malic dehydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fluorescent protein genes were high, which supported the opinion that exogenous genes could be effectively expressed in the cells. This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics, and somatic cloning.

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Bu Makaleye Atıf Yap

BAI, C., WANG, D., SU, X., ZHANG, M., GUAN, W., MA, Y. (2012). Establishment and biological research of the Jining Grey goat fibroblast line. Turkish Journal of Veterinary and Animal Sciences, Cilt: 36 - Sayı: 6, 659–667. https://ajindex.com/articles/121732

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Kaynakça (14)

  1. Ma, Y., Fen, W.: Th e evaluation of livestock and poultry breed resources. Ecol. Dom. Anim., 2002; 3: 1–5.
  2. Wu, C.: Th e theory and technology of the conservation of animal genetic resources-the specy foundation of animal agricultural continuing development in 21 century. J. Yunnan Univers., 1999; 21: 7–10.
  3. Li, L.F., Yue, H., Ma, J., Guan, W.J., Ma, Y.H.: Establishment and characterization of a fi broblast line from Simmental cattle. Cryobiology, 2009; 59: 63–68.
  4. Na, R.S., Zhao, Q.J., Jin da, P., Su, X.H., Chen, X.W., Guan, W.J., Ma, Y.H.: Establishment and biological characteristics of Ujumqin sheep fi broblast line. Cytotechnology, 2010, 62: 43–52.
  5. Li, L.F., Guan, W.J., Li, H., Zhou, X.Z., Bai, X.J., Ma, Y.H.: Establishment and characterization of a fi broblast cell line derived from Texel sheep. Biochem. Cell. Biol., 2009; 87: 1–8.
  6. Li, X.C., Yue, H., Li, C.Y., He, X.H., Zhao, Q.J., Ma, Y.H., Guan, W.J., Ma, J.Z.: Establishment and characterization of a fi broblast cell line derived from Jining Black Grey goat for genetic conservation. Small Ruminant Res., 2009; 87: 17–26.
  7. Yao, F.: Chinese Caprine resource for fur skin. Modern Livestock and Poultry Breeding Industry. 2004; 6: 44–45.
  8. Jin, J.: Rarest Jining Gray goat. Jilin J. Anim. Husb. Vet. Med., 1997; 5: 22. Freshney, R.I.: Culture of Animal Cells: A Manual of Basic Technique, 4th ed. Wiley-Liss, New York. 2000; 49–175.
  9. Guan, W.J., Ma, Y.H., Zhou, X.Y., Liu, G.L., Liu X.D.: Th e establishment of fi broblast cell line and its biological characteristic research in Taihang Black goat. Rev. China Agric. Sci. Technol., 2005; 7: 25–33.
  10. Zhou, X.M., Ma, Y.H., Guan, W.J., Zhao, D.M.: Establishment and identifi cation of Debao pony ear marginal tissue fi broblast cell line. Asian-Aust. J. Anim. Sci., 2004; 17: 1338–1343.
  11. Ren, F., Li, Y., Zhang, Y.: In vitro cultivation and freezing of bovine skin fi broblast cells. J. Yellow Cattle Sci., 2002; 28: 8–10.
  12. Freshney, R.I.: Culture of Animal Cells: A Manual of Basic Technique. 3rd ed. John Wiley & Sons, New York. 1994.
  13. Qi, Y., Tu, Y., Yang, D., Chen, Q., Xiao, J., Chen, Y., Fu, J., Xiao, X., Zhou, Z.: Cyclin A but not cyclin D1 is essential in c-myc- modulated cell-cycle progression. J. Cell. Physiol., 2007; 210: 63–71.
  14. Suemori, H., Yasuchika, K., Hasegawa, K., Fujioka, T., Tsuneyoshi, N., Nakatsuji, N.: Effi cient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage. Biochem. Biophys. Res. Commun.,