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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2013 / Cilt: 37 - Sayı: 2

Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle

Yingxia LIU
Jie CAO
Junjie ZHANG
Kai HUANG
Changming QI
Sayfa
223–225
Yayın Tarihi
2013-02-01
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus (BVDV).
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Temel Bulgular & Veri:Viral RNA was successfully extracted from blood and serum that were stored at room temperature (RT) for 7 days and was detected by 1-step RT-PCR.
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Sonuç & Katkı:The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Key words: Bovine viral diarrhea virus · viral RNA extraction · storage time

Özet

This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus (BVDV). Viral RNA was successfully extracted from blood and serum that were stored at room temperature (RT) for 7 days and was detected by 1-step RT-PCR. The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.

Abstract

This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus (BVDV). Viral RNA was successfully extracted from blood and serum that were stored at room temperature (RT) for 7 days and was detected by 1-step RT-PCR. The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.

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Bu Makaleye Atıf Yap

LIU, Y., CAO, J., ZHANG, J., HUANG, K., QI, C. (2013). Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 2, 223–225. https://ajindex.com/articles/121716

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Kaynakça (9)

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  9. Vilcek, S., Strojny, L., Durkovic, B., Rossmanith, W., Paton, D.: Storage of bovine viral diarrhea virus samples on filter paper and detection of viral RNA by a RT-PCR method. J. Virol. Methods, 2001; 92: 19–22.