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Dergiler / Turkish Journal of Veterinary and Animal Sciences / 2015 / Cilt: 39 - Sayı: 1

Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats

Alev Gürol BAYRAKTAROĞLU
Özkan ŞİMŞEK
Aytül KÜRÜM
Şevket ARIKAN
Emel ERGÜN
Sayfa
57–61
Yayın Tarihi
2015-03-15
DOI
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An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.

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Amaç & Hipotez:This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats.
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Temel Bulgular & Veri:In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study.
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Sonuç & Katkı:An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:ANAE activity · Angora cats · lymphocyte

Abstract

This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE (+) T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B lymphocytes reacted negatively in ANAE staining. The rate of ANAE (+) lymphocytes in pregnant, kitten, and adult Angora cats was 62.89 ± 1.29%, 68.37 ± 1.22%, and 77.71 ± 1.63%, respectively. In conclusion, the lowest rate of ANAE-positive lymphocytes was detected in the pregnant cats, depending on the maternal immune tolerance supported by hormonal mechanisms. An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.

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Bu Makaleye Atıf Yap

BAYRAKTAROĞLU, A. G., ŞİMŞEK, Ö., KÜRÜM, A., ARIKAN, Ş., ERGÜN, E. (2015). Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats. Turkish Journal of Veterinary and Animal Sciences, Cilt: 39 - Sayı: 1, 57–61. https://ajindex.com/articles/121683

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Kaynakça (13)

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