Rapid and simultaneous detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies by duplex PCR

Amaç & Hipotez:A rapid and effective polymerase chain reaction (PCR) test, specific for the detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies, was developed.

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Temel Bulgular & Veri:This duplex PCR technique was specifically designed to amplify the sequences of IS6110 of M. bovis and IS900 of M. avium subsp. paratuberculosis.

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Sonuç & Katkı:Therefore, this duplex PCR test is a rapid and effective technique to simultaneously detect M. bovis and M. avium subsp. paratuberculosis.

🔬Tür:Araştırma Makalesi

📍Kapsam:Genişletilmiş Akademik Örneklem

⚙️Değişkenler:Key words: Fly · Mycobacterium bovis · Mycobacterium avium subsp. paratuberculosis

Özet

A rapid and effective polymerase chain reaction (PCR) test, specific for the detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies, was developed. This duplex PCR technique was specifically designed to amplify the sequences of IS6110 of M. bovis and IS900 of M. avium subsp. paratuberculosis. The results of the interference test, simulated contamination test, and several other experiments indicated that this technique had high stability, specificity, and sensitivity with detection limits at 92.98 pg of M. bovis and 110.27 pg of M. avium subsp. paratuberculosis. Therefore, this duplex PCR test is a rapid and effective technique to simultaneously detect M. bovis and M. avium subsp. paratuberculosis.

Abstract

A rapid and effective polymerase chain reaction (PCR) test, specific for the detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies, was developed. This duplex PCR technique was specifically designed to amplify the sequences of IS6110 of M. bovis and IS900 of M. avium subsp. paratuberculosis. The results of the interference test, simulated contamination test, and several other experiments indicated that this technique had high stability, specificity, and sensitivity with detection limits at 92.98 pg of M. bovis and 110.27 pg of M. avium subsp. paratuberculosis. Therefore, this duplex PCR test is a rapid and effective technique to simultaneously detect M. bovis and M. avium subsp. paratuberculosis.

Anahtar kelimeler

Key words: Fly Mycobacterium bovis Mycobacterium avium subsp. paratuberculosis duplex PCR

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Bu Makaleye Atıf Yap

ZHANG, J., ZHENG, S., ZHU, T., LIU, S., LI, R., ZHU, T., HUANG, S., WANG, J., NI, J., ZHAO, X., SUN, J., HUANG, S. (2013). Rapid and simultaneous detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies by duplex PCR. Turkish Journal of Veterinary and Animal Sciences, Cilt: 37 - Sayı: 2, 153–159. https://ajindex.com/articles/121634

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Kaynakça (9)

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