Expression of the GPRC5D gene in Liaoning Cashmere goats

This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere growth.

Abstract

This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence was subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressed only in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere growth.

Anahtar kelimeler

GPRC5D bioinformatics RT-PCR in situ hybridization

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Bu Makaleye Atıf Yap

JIN, M., LI, X., PIAO, J., QU, Y., WANG, Y., PIAO, J., CHU, M. (2014). Expression of the GPRC5D gene in Liaoning Cashmere goats. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 4, 347–353. https://ajindex.com/articles/121600

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